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公开(公告)号：US06344350B1
公开(公告)日：2002-02-05
申请号：US09426868
申请日：1999-10-26
IPC分类号： C12N910
摘要： A novel isolate of Bacillus isolated from white pine is described. The isolate is used to produce monoterpene derivatives. Also described is a pinene hydroxylase enzyme from the isolate. The enzyme is a general allylic oxidizer.
摘要翻译： 描述了从白松木中分离的芽孢杆菌的新型分离物。 该分离物用于生产单萜衍生物。 还描述了来自分离物的蒎烯羟化酶。 该酶是通用的烯丙氧化剂。
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2.发明授权Adsorption and recovery of rifamycin B and rifamycin S using basic ion exchange resins 失效使用碱性离子交换树脂吸收和回收利福霉素B和利福霉素S
公开(公告)号：US4402877A
公开(公告)日：1983-09-06
申请号：US406659
申请日：1982-08-09
申请人： Jo Ann Gilpin , Patrick J. Oriel , Roger A. Briggs
发明人： Jo Ann Gilpin , Patrick J. Oriel , Roger A. Briggs
IPC分类号： C07D498/08 , C07D491/18 , C07D491/08
CPC分类号： C07D498/08
摘要： The use of basic ion exchange resins to adsorb rifamycin B or rifamycin S from dilute solutions containing rifamycin B or rifamycin S is disclosed. The rifamycin B or rifamycin S may be recovered from the resin by desorption with a suitable desorbing solvent system.
摘要翻译： 公开了使用碱性离子交换树脂从含有利福霉素B或利福霉素S的稀释溶液吸附利福霉素B或利福霉素S. 利福霉素B或利福霉素S可以通过用合适的解吸溶剂体系解吸从树脂中回收。
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3.发明授权Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus 失效使用来自嗜热芽孢杆菌的腈水合酶制备酰胺化合物的方法
公开(公告)号：US6153415A
公开(公告)日：2000-11-28
申请号：US248528
申请日：1999-02-10
摘要： A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60.degree. C., and is most active between 20.degree. C. to 70.degree. C. with optimum activity at 55.degree. C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
摘要翻译： 将腈转化成其相应的酰胺产物，特别是用于形成聚合物的丙烯腈至丙烯酰胺的方法。 该方法使用具有腈水合酶活性的嗜热细菌，其由钴离子组成型表达，在60℃下稳定，并且在20℃至70℃之间最为活跃，在55℃下具有最佳活性。 或者，该方法使用从嗜热细菌提取的酶将腈转化成其酰胺产物。 描述了编码腈水合酶和酰胺酶的基因，其中前者可用于将腈转化为其酰胺，并且后者可用于将酰胺转化为其酸。
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4.发明授权
公开(公告)号：US5652137A
公开(公告)日：1997-07-29
申请号：US523465
申请日：1995-09-05
申请人： Hae Choon Chang , Patrick J. Oriel
发明人： Hae Choon Chang , Patrick J. Oriel
摘要： A process for the preparation of monoterpene compounds (such as perillyl alcohol, aldehyde and .alpha.-terpineol) from limonene is described. The process uses Bacillus stearothermophilus which is effective at high temperatures (55.degree. to 70.degree. C.).
摘要翻译： 描述了从柠檬烯制备单萜化合物（例如紫苏醇，醛和α-萜品醇）的方法。 该方法使用在高温（55〜70℃）下有效的嗜热脂肪芽孢杆菌。
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5.发明授权公开(公告)号：US06242242B1
公开(公告)日：2001-06-05
申请号：US09549106
申请日：2000-04-13
IPC分类号： C12N120
摘要： A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
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6.发明授权Process for the preparation of monoterpenes using bacterium containing recombinant DNA 失效使用含有重组DNA的细菌制备单萜的方法
公开(公告)号：US5688673A
公开(公告)日：1997-11-18
申请号：US508818
申请日：1995-07-28
摘要： A process for producing monoterpenes using a DNA segment Bacillus stearothermophilus in a recombinant bacterium, particularly Escherichia coli. The useful monoterpenes produced from limonene are perillyl alcohol, perillyl aldehyde, perillic acid (path A); alpha-terpineol (path B) or carveol and carvone (path C).
摘要翻译： 在重组细菌，特别是大肠杆菌中使用DNA片段嗜热脂肪芽孢杆菌生产单萜的方法。 由柠檬烯生产的有用的单萜是香叶醇，香草醛，紫苏酸（路径A）; α-萜品醇（路径B）或香芹酮（C）。
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公开(公告)号：US5571887A
公开(公告)日：1996-11-05
申请号：US492318
申请日：1995-06-19
申请人： Li-Xia Li , Patrick J. Oriel , Eric A. Grulke
发明人： Li-Xia Li , Patrick J. Oriel , Eric A. Grulke
摘要： A new thermoplastic homopolymer of o-cresol which is a poly(2-methylphenylene oxide) having a high molecular weight distribution is described. The polymer is prepared using a 2 and/or 6 substituted pyridine catalyst to the molecular weight distribution. The homopolymer can be blended with other thermoplastic polymers. The homopolymer is rapidly biodegraded, particularly in soil containing soil microorganisms.
摘要翻译： 描述了作为高分子量分布的聚（2-甲基亚苯基氧化物）的新型的甲酚的热塑性均聚物。 使用2和/或6取代的吡啶催化剂制备聚合物至分子量分布。 均聚物可与其它热塑性聚合物共混。 均聚物快速生物降解，特别是在含有土壤微生物的土壤中。
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8.发明授权公开(公告)号：US06228633B1
公开(公告)日：2001-05-08
申请号：US09549111
申请日：2000-04-13
IPC分类号： C12N988
摘要： A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
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9.发明授权Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus 失效使用来自嗜热芽孢杆菌的腈水合酶制备酰胺化合物的方法公开(公告)号：US06214603B1
公开(公告)日：2001-04-10
申请号：US09549108
申请日：2000-04-13
IPC分类号： C12N120
摘要： A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
摘要翻译： 将腈转化成其相应的酰胺产物，特别是用于形成聚合物的丙烯腈至丙烯酰胺的方法。 该方法使用具有腈水合酶活性的嗜热细菌，其由钴离子组成型表达，在60℃下稳定，并且在20℃至70℃之间最为活跃，在55℃具有最佳活性。 或者，该方法使用从嗜热细菌提取的酶将腈转化成其酰胺产物。 描述了编码腈水合酶和酰胺酶的基因，其中前者可用于将腈转化为其酰胺，并且后者可用于将酰胺转化为其酸。
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公开(公告)号：US5763237A
公开(公告)日：1998-06-09
申请号：US857873
申请日：1997-05-16
CPC分类号： C12P7/02 , Y10S435/832
摘要： Enzymatic microbial degradation of limonene with simultaneous extraction of the degradation products with a non-water miscible organic solvent is described. Microbial degradation at elevated temperatures employing both an aqueous phase containing limonene and a neat limonene phase produced .alpha.-terpineol with additional production of carvone.
摘要翻译： 描述了用非水混溶性有机溶剂同时提取降解产物的柠檬烯的酶促微生物降解。 使用包含柠檬烯和纯柠檬烯相的水相在高温下的微生物降解产生α-萜品醇，另外产生香芹酮。
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