公开(公告)号：US20070031454A1

公开(公告)日：2007-02-08

申请号：US11494825

申请日：2006-07-28

申请人： David Lowery ,  Sing Rong ,  Paul Guimond ,  Paula Clare ,  Cassius Tucker ,  Thomas Newby

发明人： David Lowery ,  Sing Rong ,  Paul Guimond ,  Paula Clare ,  Cassius Tucker ,  Thomas Newby

IPC分类号： A61K39/125 ,  A61K48/00

CPC分类号： C07K16/10 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/70 ,  C07K14/005 ,  C07K2317/76 ,  C12N7/00 ,  C12N2710/16734 ,  C12N2750/10034 ,  C12N2750/14334 ,  C12N2770/16021 ,  C12N2770/16022 ,  C12N2770/16034 ,  G01N33/56983 ,  G01N2469/10

摘要： The present invention relates to a vaccine for immunizing a cat against feline viruses. The present invention also relates to a nucleic acid clone that encodes the capsid protein of the isolated feline calicivirus. The present invention further relates to a live or killed vaccine comprising the isolated feline calicivirus, a subunit vaccine comprising the capsid protein of the isolated feline calicivirus, a nucleic acid vaccine comprising a nucleic acid clone of the isolated feline calicivirus, and a recombinant virus vector vaccine comprising nucleic acid encoding the capsid protein of the isolated feline calicivirus. The present invention also relates to a method for identifying a feline calicivirus useful for producing a vaccine composition and for assays for diagnosing cats infected with feline calicivirus. Also disclosed is a method of immunizing animals, especially cats, against disease, in particular against feline calicivirus (FCV). The method includes administering to a cat therapeutically effective amounts of first and second FCV vaccines. The first vaccine is administered orally or parenterally (e.g., subcutaneously, intramuscularly, and the like). The second vaccine is administered orally or oronasally N days following administration of the first vaccine, wherein N is an integer from 3 to 120, inclusive. A third vaccine administration may also be given. The present invention also describes methods and materials for treating and immunizing animals with vaccine, and in particular cats against both FPV or Feline Parvovirus, which has also been called Panleukopenia or FPL and against another disease, FHV or Feline Herpes Virus, which has also been called Feline Rhinotracheitis Virus.

摘要翻译： 本发明涉及用于免疫猫对猫科动物病毒的疫苗。 本发明还涉及编码分离的猫杯状病毒的衣壳蛋白的核酸克隆。 本发明还涉及包含分离的猫杯状病毒的活的或杀死的疫苗，包含分离的猫杯状病毒的衣壳蛋白的亚单位疫苗，包含分离的猫杯状病毒的核酸克隆的核酸疫苗和重组病毒载体 包含编码分离的猫杯状病毒的衣壳蛋白的核酸的疫苗。 本发明还涉及用于鉴定可用于产生疫苗组合物的猫杯状病毒病的方法和用于诊断感染猫杯状病毒的猫的测定方法。 还公开了免疫动物特别是猫的疾病，特别是针对猫杯状病毒（FCV）的方法。 该方法包括给予猫治疗有效量的第一和第二FCV疫苗。 第一种疫苗口服或肠胃外给药（例如皮下，肌肉内等）。 第二种疫苗在第一种疫苗给药后N天口服或鼻道给药，其中N为3至120的整数，包括端值在内。 也可以给予第三次疫苗管理。 本发明还描述了用疫苗治疗和免疫动物的方法和材料，特别是针对FPV或猫细小病毒的猫，其也称为泛素减少或FPL，并且与另一种疾病FHV或猫疱疹病毒 称为猫科鼻气管炎病毒。

公开(公告)号：US07160544B2

公开(公告)日：2007-01-09

申请号：US10705660

申请日：2003-11-10

申请人： Terry L. Bowersock ,  Paul Guimond ,  Tzu-Chi R. Ju ,  Argaw Kidane

发明人： Terry L. Bowersock ,  Paul Guimond ,  Tzu-Chi R. Ju ,  Argaw Kidane

IPC分类号： A61K9/14

CPC分类号： A61K39/39 ,  A61K9/1641 ,  A61K9/1652 ,  A61K38/00 ,  A61K2039/541 ,  A61K2039/542 ,  A61K2039/55555 ,  A61K2039/55566

摘要： The invention provides a vaccine composition and a method of preparation including the steps of: forming a water-in-oil emulsion including an alginate in water, an oil, an antigen, and either (a) a cellulose ether and at least one nonionic surfactant or (b) a PEO-PPO-PEO triblock copolymer surfactant and at least one nonionic surfactant; followed by crosslinking the alginate in the emulsion with at least two cations selected from the group consisting of aluminum, barium, calcium, lithium, manganese, strontium, and zinc, to form antigen-containing, crosslinked alginate microparticles; and harvesting the microparticles. Another aspect of the invention is a method of vaccinating a vertebrate species including the step of administering to the species a vaccine composition prepared according to the method of the invention. The compositions of the invention have improved antigen loading, reduced microparticle size, increased hydrophobicity, improved uptake by antigen sampling cells, controlled antigen release characteristics, and improved immunogenicity.

摘要翻译： 本发明提供疫苗组合物和制备方法，包括以下步骤：在水，油，抗原中形成包含藻酸盐的油包水乳液，和（a）纤维素醚和至少一种非离子表面活性剂 或（b）PEO-PPO-PEO三嵌段共聚物表面活性剂和至少一种非离子表面活性剂; 然后用至少两种选自铝，钡，钙，锂，锰，锶和锌的阳离子将乳液中的藻酸盐交联，形成含抗原的交联藻酸盐微粒; 并收获微粒。 本发明的另一方面是疫苗接种脊椎动物物种的方法，包括向物种施用根据本发明方法制备的疫苗组合物的步骤。 本发明的组合物具有改善的抗原负载，降低的微粒尺寸，增加的疏水性，改善抗原取样细胞的摄取，受控的抗原释放特征和改善的免疫原性。

公开(公告)号：US06656470B2

公开(公告)日：2003-12-02

申请号：US09853919

申请日：2001-05-11

申请人： Terry L. Bowersock ,  Paul Guimond ,  Tzu-Chi R. Ju ,  Argaw Kidane

发明人： Terry L. Bowersock ,  Paul Guimond ,  Tzu-Chi R. Ju ,  Argaw Kidane

IPC分类号： A61K914

CPC分类号： A61K39/39 ,  A61K9/1641 ,  A61K9/1652 ,  A61K38/00 ,  A61K2039/541 ,  A61K2039/542 ,  A61K2039/55555 ,  A61K2039/55566

摘要： The invention provides a vaccine composition and a method of preparation including the steps of: forming a water-in-oil emulsion including an alginate in water, an oil, an antigen, and either (a) a cellulose ether and at least one nonionic surfactant or (b) a PEO-PPO-PEO triblock copolymer surfactant and at least one nonionic surfactant; followed by crosslinking the alginate in the emulsion with at least two cations selected from the group consisting of aluminum, barium, calcium, lithium, manganese, strontium, and zinc, to form antigen-containing, crosslinked alginate microparticles; and harvesting the microparticles. Another aspect of the invention is a method of vaccinating a vertebrate species including the step of administering to the species a vaccine composition prepared according to the method of the invention. The compositions of the invention have improved antigen loading, reduced microparticle size, increased hydrophobicity, improved uptake by antigen sampling cells, controlled antigen release characteristics, and improved immunogenicity.