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    USES OF THE PSEUDOMONAS SYRINGAE EFFECTOR PROTEIN HopU1 RELATED TO ITS ABILITY TO ADP-RIBOSYLATE EUKARYOTIC RNA BINDING PROTEINS
    3.
    发明申请
    USES OF THE PSEUDOMONAS SYRINGAE EFFECTOR PROTEIN HopU1 RELATED TO ITS ABILITY TO ADP-RIBOSYLATE EUKARYOTIC RNA BINDING PROTEINS 审中-公开
    PSEUDOMONAS SYRINGAE EFFECTOR蛋白HopU1的使用与其对ADP-二磷酸核糖核酸结合蛋白的能力相关

    公开(公告)号：US20080028487A1

    公开(公告)日：2008-01-31

    申请号：US11759054

    申请日：2007-06-06

    申请人： JAMES ALFANO ,  ZHENG QING FU ,  THOMAS ELTHON

    发明人： JAMES ALFANO ,  ZHENG QING FU ,  THOMAS ELTHON

    IPC分类号： A01H1/00 ,  C12Q1/68

    CPC分类号： C07K14/21 ,  C12N15/8279 ,  C12N15/8281

    摘要： The bacterial plant pathogen Pseudomonas syringae injects effector proteins into host cells via a type III protein secretion system to cause disease. The invention relates to the discovery that the type III effector HopU1 is a mono-ADP-ribosyltransferase (ADP-RT) and suppresses plant innate immunity. The HopU1 substrates in Arabidopsis thaliana extracts were RNA-binding proteins that possess RNA-recognition motifs (RRMs). A. thaliana knock-out lines defective in the glycine-rich RNA-binding protein AtGRP7, a HopU1 substrate, were more susceptible than wild type plants to P. syringae. The ADP-ribosylation of AtGRP7 by HopU1 required two arginines within the RRM. The invention provides novel methods for the modulation of the innate immune response of a plant to a biotic stress, including methods for enhancing or suppressing the innate immune response of the plant.

    摘要翻译： 细菌植物病原体丁香假单胞菌通过III型蛋白质分泌系统将效应子蛋白注入宿主细胞，引起疾病。 本发明涉及III型效应物HopU1是单ADP-核糖基转移酶（ADP-RT）并抑制植物先天免疫的发现。 拟南芥提取物中的HopU1底物是具有RNA识别基序（RRM）的RNA结合蛋白。 在富含甘氨酸的RNA结合蛋白AtGP7（HopU1底物）中缺失的拟南芥敲除系比野生型植物对丁香假单胞菌更敏感。 通过HopU1的AtGRP7的ADP-核糖基化需要RRM内的两个精氨酸。 本发明提供了用于调节植物对生物胁迫的先天免疫应答的新方法，包括用于增强或抑制植物的先天免疫应答的方法。

    Monitoring signal-to-noise ratio in x-ray diffraction data
    4.
    发明申请
    Monitoring signal-to-noise ratio in x-ray diffraction data 审中-公开
    监测x射线衍射数据中的信噪比

    公开(公告)号：US20060067470A1

    公开(公告)日：2006-03-30

    申请号：US10520777

    申请日：2003-07-14

    申请人： Bi-Cheng Wang ,  Zheng-Qing Fu ,  John Rose

    发明人： Bi-Cheng Wang ,  Zheng-Qing Fu ,  John Rose

    IPC分类号： G01N23/207

    CPC分类号： G01N23/207 ,  G01N23/2076

    摘要： The present invention relates to methods of diffractometrically determining the structures of materials by characterizing their electron density distributions. More particularly, the present invention relates to methods of collecting, processing and interpreting X-ray diffraction data, which allow real time evaluation of the signal-to-noise ratio in crystal diffraction experiments. The present methods related to the derivation of statistical indices for monitoring and evaluating signal-to-noise ratios in diffraction experiments. In addition, the present invention provides methods of determining the electron density distributions of crystals using anomalous scattering signals corrected for noise. Further, the present invention provides methods of increasing the signal-to-noise ratios in X-ray diffraction data.

    摘要翻译： 本发明涉及通过表征其电子密度分布来衍射材料的结构的方法。 更具体地，本发明涉及收集，处理和解释X射线衍射数据的方法，其允许在晶体衍射实验中实时评估信噪比。 本方法与衍射实验中监测和评估信噪比的统计指标的推导有关。 此外，本发明提供了使用针对噪声进行校正的异常散射信号来确定晶体的电子密度分布的方法。 此外，本发明提供了增加X射线衍射数据中的信噪比的方法。