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    GLYCINE RIBOSWITCHES, METHODS FOR THEIR USE, AND COMPOSITIONS FOR USE WITH GLYCINE RIBOSWITCHES
    1.
    发明申请
    GLYCINE RIBOSWITCHES, METHODS FOR THEIR USE, AND COMPOSITIONS FOR USE WITH GLYCINE RIBOSWITCHES 审中-公开
    谷氨酰胺糖苷，其使用方法和用于糖蛋白酶的组合物

    公开(公告)号：US20130029342A1

    公开(公告)日：2013-01-31

    申请号：US13563407

    申请日：2012-07-31

    申请人： Ronald R. Breaker ,  Jeffrey Barrick ,  Maumita Mandal

    发明人： Ronald R. Breaker ,  Jeffrey Barrick ,  Maumita Mandal

    IPC分类号： C12N15/113 ,  C12Q1/68 ,  C12N15/63

    CPC分类号： C12N15/85 ,  C12N15/115 ,  C12N15/67 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2840/002 ,  C12N2840/102 ,  C12N2840/55

    摘要： Riboswitches are structural elements in mRNA that change state when bound by a trigger molecule, and are thus able to regulate gene expression. They can be dissected into two separate domains: one that selectively binds the target (aptamer domain) and another that influences genetic control (expression platform domain). Bacterial glycine riboswitches consist of two tandem aptamer domains which cooperatively bind glycine to regulate the expression of downstream genes. These natural switches are targets for antibiotics and other small molecule therapies. Modified versions of these natural riboswitches can be employed as designer genetic switches that are controlled by specific effector compounds. Disclosed are isolated and recombinant riboswitches, and compositions and methods for selecting and identifying compounds that can activate, inactivate, or block a riboswitch.

    摘要翻译： 核糖开关是mRNA中的结构元件，其在被触发分子结合时改变状态，因此能够调节基因表达。 它们可以分为两个独立的领域：选择性结合目标（适体结构域）和影响遗传控制（表达平台结构域）的另一个结构域。 细菌甘氨酸核糖开关由两个串联适体结构域组成，它们协同结合甘氨酸以调节下游基因的表达。 这些天然开关是抗生素和其他小分子治疗的靶标。 这些天然核糖开关的修饰版本可以用作由特异性效应子化合物控制的设计者遗传开关。 公开了分离和重组的核糖开关，以及用于选择和鉴定可以激活，灭活或阻断核糖开关的化合物的组合物和方法。

    PREQ1 RIBOSWITCHES AND METHODS AND COMPOSITIONS FOR USE OF AND WITH PREQ1 RIBOSWITCHES
    2.
    发明申请
    PREQ1 RIBOSWITCHES AND METHODS AND COMPOSITIONS FOR USE OF AND WITH PREQ1 RIBOSWITCHES 审中-公开
    PREQ1 RIBOSWITCHES和使用和与PREQ1 RIBOSWITCHES的方法和组合物

    公开(公告)号：US20130012527A1

    公开(公告)日：2013-01-10

    申请号：US13617993

    申请日：2012-09-14

    申请人： Ronald R. Breaker ,  Jeffrey E. Barrick ,  Adam Roth ,  Wade Winkler

    发明人： Ronald R. Breaker ,  Jeffrey E. Barrick ,  Adam Roth ,  Wade Winkler

    IPC分类号： C12N15/113 ,  C12Q1/02 ,  C12P17/18 ,  C12Q1/68 ,  A01N43/90 ,  A61K31/519 ,  A61P31/04 ,  A01P1/00 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N15/63

    摘要： The preQ1 riboswitch is a target for antibiotics and other small molecule therapies. The preQ1 riboswitch and portions thereof can be used to regulate the expression or function of RNA molecules and other elements and molecules. The preQ1 riboswitch and portions thereof can be used in a variety of other methods to, for example, identify or detect compounds. Compounds can be used to stimulate, active, inhibit and/or inactivate the preQ1 riboswitch. The preQ1 riboswitch and portions thereof, both alone and in combination with other nucleic acids, can be used in a variety of constructs and RNA molecules and can be encoded by nucleic acids.

    摘要翻译： preQ1核糖开关是抗生素和其他小分子治疗的靶标。 preQ1核糖开关及其部分可用于调节RNA分子和其他元件和分子的表达或功能。 preQ1核糖开关及其部分可用于各种其它方法中，以便例如鉴定或检测化合物。 化合物可用于刺激，活化，抑制和/或灭活前QI核糖开关。 preQ1核糖开关及其部分单独和与其它核酸组合可用于多种构建体和RNA分子中，并可由核酸编码。

    Enzymatic nucleic acid molecules
    4.
    发明授权
    Enzymatic nucleic acid molecules 有权
    酶的核酸分子

    公开(公告)号：US07807817B2

    公开(公告)日：2010-10-05

    申请号：US11605177

    申请日：2006-11-28

    申请人： Gerald F. Joyce ,  Ronald R. Breaker

    发明人： Gerald F. Joyce ,  Ronald R. Breaker

    IPC分类号： C07H21/04 ,  A61K31/70

    CPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q2521/337

    摘要： The present invention discloses nucleic acid enzymes and deoxyribonucleic acid enzymes capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a site-specific manner, as well as compositions including same. Methods of making and using the disclosed enzymes and compositions are also disclosed.

    摘要翻译： 本发明公开了能够以位点特异性方式切割核酸序列或分子，特别是RNA的核酸酶和脱氧核糖核酸酶，以及包含其的组合物。 还公开了制备和使用所公开的酶和组合物的方法。

    Riboswitches, methods for their use, and compositions for use with riboswitches
    5.
    发明授权
    Riboswitches, methods for their use, and compositions for use with riboswitches 有权
    核糖开关，其使用方法和用于核糖开关的组合物

    公开(公告)号：US07794931B2

    公开(公告)日：2010-09-14

    申请号：US10669162

    申请日：2003-09-22

    申请人： Ronald R. Breaker ,  Ali Nahvi ,  Narasimhan Sudarsan ,  Margaret S. Ebert ,  Wade Winkler ,  Jeffrey E. Barrick ,  John K. Wickiser

    发明人： Ronald R. Breaker ,  Ali Nahvi ,  Narasimhan Sudarsan ,  Margaret S. Ebert ,  Wade Winkler ,  Jeffrey E. Barrick ,  John K. Wickiser

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/113 ,  A61K31/00 ,  C07D415/00 ,  C07D473/18 ,  C07D473/34 ,  C12N15/115 ,  C12N15/67 ,  C12N2310/53

    摘要： It has been discovered that certain natural mRNAs serve as metabolite-sensitive genetic switches wherein the RNA directly binds a small organic molecule. This binding process changes the conformation of the mRNA, which causes a change in gene expression by a variety of different mechanisms. Modified versions of these natural “riboswitches” (created by using various nucleic acid engineering strategies) can be employed as designer genetic switches that are controlled by specific effector compounds. Such effector compounds that activate a riboswitch are referred to herein as trigger molecules. The natural switches are targets for antibiotics and other small molecule therapies. In addition, the architecture of riboswitches allows actual pieces of the natural switches to be used to construct new non-immunogenic genetic control elements, for example the aptamer (molecular recognition) domain can be swapped with other non-natural aptamers (or otherwise modified) such that the new recognition domain causes genetic modulation with user-defined effector compounds. The changed switches become part of a therapy regimen—turning on, or off, or regulating protein synthesis. Newly constructed genetic regulation networks can be applied in such areas as living biosensors, metabolic engineering of organisms, and in advanced forms of gene therapy treatments.

    摘要翻译： 已经发现某些天然mRNA用作代谢物敏感的遗传开关，其中RNA直接结合小的有机分子。 这种结合过程改变mRNA的构象，其通过各种不同的机制引起基因表达的改变。 这些天然“核糖开关”（通过使用各种核酸工程策略产生）的修饰版本可被用作由特异性效应子化合物控制的设计者遗传开关。 激活核糖开关的这种效应子化合物在本文中称为触发分子。 天然开关是抗生素和其他小分子治疗的靶标。 此外，核糖开关的结构允许使用自然开关的实际部分来构建新的非免疫原性遗传控制元件，例如适配子（分子识别）结构域可以与其他非天然适体（或以其它方式修饰）交换， 使得新的识别域引起用户定义的效应子化合物的遗传调节。 改变的开关成为治疗方案的一部分 - 打开或关闭或调节蛋白质合成。 新建的遗传调控网络可以应用于生物传感器，生物体的代谢工程和基因治疗治疗的先进形式等领域。

    Enzymatic DNA molecules
    7.
    发明授权
    Enzymatic DNA molecules 失效
    酶DNA分子

    公开(公告)号：US06326174B1

    公开(公告)日：2001-12-04

    申请号：US08849567

    申请日：1997-08-25

    申请人： Gerald F. Joyce ,  Ronald R. Breaker

    发明人： Gerald F. Joyce ,  Ronald R. Breaker

    IPC分类号： C12P1934

    CPC分类号： C12Q1/68 ,  C12N15/113 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2320/10 ,  C12N2320/13 ,  C12Q1/6811

    摘要： The present invention discloses deoxyribonucleic acid enzymes—catalytic or enzymatic DNA molecules—capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a site-specific manner, as well as compositions including same. Methods of making and using the disclosed enzymes and compositions are also disclosed.

    摘要翻译： 本发明公开了能够以位点特异性方式切割核酸序列或分子，特别是RNA的催化或酶DNA分子以及包含其的组合物的脱氧核糖核酸酶。 还公开了制备和使用所公开的酶和组合物的方法。

    STRUCTURED RNA MOTIFS AND COMPOUNDS AND METHODS FOR THEIR USE
    9.
    发明申请
    STRUCTURED RNA MOTIFS AND COMPOUNDS AND METHODS FOR THEIR USE 审中-公开
    结构化RNA的MOTIFS和化合物及其使用方法

    公开(公告)号：US20120321647A1

    公开(公告)日：2012-12-20

    申请号：US13521685

    申请日：2011-01-12

    申请人： Ronald R. Breaker ,  Zasha Weinberg

    发明人： Ronald R. Breaker ,  Zasha Weinberg

    IPC分类号： A61K31/7076 ,  C07H21/02 ,  A61K31/197 ,  C12N1/20 ,  A61K39/40 ,  A61P31/04 ,  C12N15/63 ,  C12Q1/68

    CPC分类号： C12N15/67 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/17

    摘要： Disclosed are compositions and methods involing riboswitches and RNA motifs. For example, disclosed are compositions and methods involving glutamine-responsive riboswitches, S-adenosylmethionine-repsonsive riboswitches, S-adenosylhomocysteine-repsonsive riboswitches, glutamine riboswitches, SAM/SAH riboswitches, glnA riboswitches, Downstream-peptide riboswitches, crcB riboswitches, pfl riboswitches, yjdF riboswitches, manA riboswitches, wcaG riboswitches, epsC riboswitches, ykkC-III riboswitches, psaA riboswitches, psbA riboswitches, PhotoRC-I riboswitches, PhotoRC-II riboswitches, and psbNH riboswitches.

    摘要翻译： 公开了包含核糖开关和RNA基序的组合物和方法。 例如，所公开的是涉及谷氨酰胺反应性核糖开关，S-腺苷甲硫氨酸代谢核糖开关，S-腺苷高半胱氨酸代谢核糖开关，谷氨酰胺核糖开关，SAM / SAH核糖开关，glnA核糖开关，下游肽核糖开关，crcB核糖开关，pfl核糖开关， yjdF核糖开关，manA核糖开关，wcaG核糖开关，epsC核糖开关，ykkC-III核糖开关，psaA核糖开关，psbA核糖开关，PhotoRC-I核糖开关，PhotoRC-II核糖开关和psbNH核糖开关。