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公开(公告)号：US4635488A
公开(公告)日：1987-01-13
申请号：US677226
申请日：1984-12-03
申请人： Richard D. Kremer
发明人： Richard D. Kremer
IPC分类号： A61B10/00 , G01N1/10 , G01N1/14 , G01N33/487 , G01N1/12
CPC分类号： A61B10/0045 , G01N1/10 , A61B10/0051 , A61B10/0064 , A61B2010/0006 , A61B2010/0009 , A61B2010/0067 , B01L2300/069 , B01L2300/161 , B01L2400/0406 , B01L2400/0457 , B01L3/5029 , G01N2001/149 , G01N33/487
摘要： A body fluid sampling device including a hollow tube with a solid, porous, water-wettable nonfibrous nib mounted in and protruding from one end of the tube for collecting, by absorption, a sample of a body fluid such as sweat, tears, or saliva. The sample may be extracted from the nib for analysis by supplying an extraction fluid to the interior of the tube for gravity or vacuum-assisted flow out through the nib. Alternatively, an elongated analysis element such as a strip of paper or a packed column, e.g. containing an agent that changes appearance to indicate the presence of a substance to be detected, may be disposed in the tube for endwise contact with the nib to receive the sample (or components thereof) by absorption.
摘要翻译： 一种体液取样装置，包括一个中空管，该中空管具有固定的，多孔的，水润湿的非纤维笔尖，其安装在该管的一端并从该管的一端突出，用于通过吸收来收集诸如汗液，泪液或唾液的体液样本 。 可以从笔尖中提取样品用于分析，通过将提取流体提供到管的内部用于重力或通过笔尖的真空辅助流出。 或者，细长的分析元件，例如纸条或填充柱，例如。 含有改变外观以指示待检测物质的存在的试剂可以设置在管中以与笔尖端接触以通过吸收接收样品（或其组分）。
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2.发明授权Methods and devices for collecting and storing clinical samples for genetic analysis 失效用于收集和存储用于遗传分析的临床样品的方法和装置公开(公告)号：US06168922A
公开(公告)日：2001-01-02
申请号：US09255151
申请日：1999-02-22
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , B01J2219/00639 , B01J2219/00722 , C12N15/1003 , C40B40/06 , C12Q2565/625 , C12Q2527/127
摘要： The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, or purify nucleic acids either from a biological source other than untreated whole blood, the biological source having naturally occurring nucleic acid amplification inhibitors present, (including either a buccal swab, cerebrospinal fluid, feces, lymphatic fluid, a plasma sample, a saliva sample, a serum sample, urine, or a suspension of cells or viruses), or from a treated whole blood source that has naturally occurring nucleic acid amplification inhibitors present, as well as added blood stabilization components that also inhibit nucleic acid amplification. More importantly, these nucleic acids can be released after collection or storage in a manner that enables them to be amplified by conventional techniques such as polymerase chain reaction. In particular, an absorbent material that does not bind nucleic acids irreversibly is impregnated with a chaotropic salt. A biological source sample is contacted with the impregnated absorbent material. Any nucleic acids present in the biological source can be either eluted or resolubilized off the absorbent material.
摘要翻译： 本发明涉及用于从流体样品中收集，储存和纯化来自流体样品的核酸的装置和方法，用于随后的遗传表征，主要通过常规的扩增方法。 本发明可用于从除未经处理的全血之外的生物来源收集，储存或纯化核酸，所述生物来源具有天然存在的核酸扩增抑制剂（包括颊拭子，脑脊液，粪便， 淋巴液，血浆样品，唾液样品，血清样品，尿液或细胞或病毒的悬浮液）或来自具有天然存在的核酸扩增抑制剂的处理的全血源，以及添加的血液稳定化成分 这也抑制核酸扩增。 更重要的是，这些核酸可以在收集或储存之后以能够通过常规技术如聚合酶链反应扩增的方式被释放。 特别地，不结合核酸的吸收材料不可逆地浸渍有离液盐。 生物源样品与浸渍的吸收材料接触。 存在于生物来源中的任何核酸都可以从吸收材料中洗脱或再溶解。
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公开(公告)号：US4272381A
公开(公告)日：1981-06-09
申请号：US87074
申请日：1979-10-22
CPC分类号： G01N30/91 , G01N30/6065
摘要： A device for spotting samples at spaced locations on the adsorbent surface of a TLC (thin-layer chromatography) plate has a base with an elevated, flat TLC plate supporting area and an adjacent stepped down solvent trough containing area separated by a step. A wicking plate, having a number of wick-holding wells, is adapted to be positioned over the trough with the wicks dipping in the trough and the upper ends of the wicks adapted to engage the adsorbent surface of a TLC plate lowered against them. A novel wick of porous polyethylene may be pre-loaded with sample and dried before being loaded into the wicking plate. The base has spaced extensions defining a cut-out over which the wicking plate can be laid for loading, being appropriately positioned by pins extending from one of its long sides engaging the step on the base. When reversed, the non-pin-containing long side of the wicking plate engages the step and positions it over the trough. The invention includes the novel method of spotting a TLC plate employing the new device.
摘要翻译： 用于在TLC（薄层色谱）板的吸附剂表面上的间隔位置处点样品的装置具有基底，其具有升高的平坦TLC板支撑区域和相邻的逐步降低的溶剂槽容纳区域被步骤分离。 具有许多芯持有孔的吸液板适于定位在槽的上方，其中芯浸在槽中，并且芯的上端适于接合降低到其的TLC板的吸附剂表面。 多孔聚乙烯的新型芯可以预先装载样品并在装入芯吸板之前干燥。 底座具有限定切口的间隔开的延伸部分，芯吸板可以放置在该切口上，用于装载，通过从其与底座上的台阶接合的长边中的一个延伸的销适当地定位。 当反转时，芯吸板的非针脚长边接合台阶并将其定位在槽上。 本发明包括采用新装置点样TLC板的新方法。
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公开(公告)号：US4077875A
公开(公告)日：1978-03-07
申请号：US764439
申请日：1977-01-31
申请人： Richard D. Kremer
发明人： Richard D. Kremer
CPC分类号： B01D61/28 , B01D61/145 , B01D61/18 , B01D63/06
摘要： Ultrafiltration apparatus employing collodion bag dialyzing means. This apparatus provides a means for accelerating the dialysis process and a cooperative means for obtaining samples of the concentrate from the collodion bag. A displacement body with an axial bore is attached to a tube and positioned within the collodion bag. By displacing fluid volume in the bag, the speed of dialysis is increased. The combination of the tube and displacement body also facilitates removal of samples of the concentrated fluid from the bag.
摘要翻译： 超滤设备采用电话袋式透析方式。 该装置提供了一种用于加速透析过程的手段，以及用于从胶卷袋获得浓缩物样品的合作手段。 具有轴向孔的位移体附接到管并定位在火棉袋内。 通过移动袋中的流体体积，透析速度增加。 管和位移体的组合也有助于从袋中去除浓缩流体的样品。
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5.发明授权Methods and devices for collecting and storing clinical samples for genetic analysis 失效用于收集和存储用于遗传分析的临床样品的方法和装置
公开(公告)号：US5939259A
公开(公告)日：1999-08-17
申请号：US835614
申请日：1997-04-09
CPC分类号： C12Q1/6806 , C12N15/1003 , B01J2219/00639 , B01J2219/00722 , C40B40/06
摘要： The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, or purify nucleic acids from a treated whole blood source that has naturally occurring nucleic acid amplification inhibitors present, as well as added blood stabilization components that also inhibit nucleic acid amplification. More importantly, these nucleic acids can be released after collection or storage in a manner that enables them to be amplified by conventional techniques such as polymerase chain reaction. In particular, an absorbent material that does not bind nucleic acids irreversibly is impregnated with a chaotropic salt. A biological source sample is contacted with the impregnated absorbent material. Any nucleic acids present in the biological source can be either eluted or resolubilized off the absorbent material.
摘要翻译： 本发明涉及用于从流体样品中收集，储存和纯化来自流体样品的核酸的装置和方法，用于随后的遗传表征，主要通过常规的扩增方法。 本发明可用于从存在天然存在的核酸扩增抑制剂的经处理的全血源收集，储存或纯化核酸，以及还抑制核酸扩增的添加的血液稳定化成分。 更重要的是，这些核酸可以在收集或储存之后以能够通过常规技术如聚合酶链反应扩增的方式被释放。 特别地，不结合核酸的吸收材料不可逆地浸渍有离液盐。 生物源样品与浸渍的吸收材料接触。 存在于生物来源中的任何核酸都可以从吸收材料中洗脱或再溶解。
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公开(公告)号：US4534863A
公开(公告)日：1985-08-13
申请号：US612905
申请日：1984-05-22
CPC分类号： B01L3/5021 , B01D29/01 , B01L3/5635 , B01L2200/023 , B01L2200/026 , B01L2200/0689 , B01L2300/042 , B01L2300/0681 , B01L2300/126 , B01L2400/0409
摘要： A centrifugal filtering device including a filter unit comprising an open-ended sample tube; a filter support element, with a central open-ended passage, ultrasonically welded to the outlet end of the sample tube at an annular joint and having a seat at its outer end; and a filter clamped between the sample tube and the support element, through which liquid is forced from the sample tube when the device is centrifuged. The complete device also includes a receiving vessel, for collecting filtered liquid, removably mounted on the support element seat, and a cap for the inlet end of the sample tube. The annular weld joint is made relatively weak to enable separation of the support element and sample tube for recovery of the filter. The sample tube, support element and receiver vessel are made of polypropylene. To maximize fluid recovery, the inner wall of the sample tube tapers inwardly to the outlet opening of the tube, and the support element seat is arranged to mount either of two different sizes of receiver vessels.
摘要翻译： 一种离心过滤装置，包括：包括开放式样品管的过滤器单元; 具有中央开口通道的过滤器支撑元件，其在环形接头处超声波焊接到样品管的出口端，并在其外端具有座; 以及被夹持在样品管和支撑元件之间的过滤器，当离心该装置时液体被从样品管中被迫使。 整个装置还包括用于收集过滤液体的接收容器，可拆卸地安装在支撑元件座上，以及用于样品管入口端的盖。 使环形焊接接头相对较弱，以使分离支撑元件和样品管以恢复过滤器。 样品管，支撑元件和接收容器由聚丙烯制成。 为了使液体回收最大化，样品管的内壁向内逐渐变细到管的出口，并且支撑元件座被布置成安装两种不同尺寸的接收容器之一。
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