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    Polymer-based protein engineering methods to rationally tune enzyme activity, pH-dependence and stability
    2.
    发明授权
    Polymer-based protein engineering methods to rationally tune enzyme activity, pH-dependence and stability 有权
    基于聚合物的蛋白质工程方法合理调节酶活性，pH依赖性和稳定性

    公开(公告)号：US09539338B2

    公开(公告)日：2017-01-10

    申请号：US14785868

    申请日：2014-04-22

    申请人： Alan J. Russell ,  Richard R. Koepsel ,  Chad Cummings ,  Hironobu Murata

    发明人： Alan J. Russell ,  Richard R. Koepsel ,  Chad Cummings ,  Hironobu Murata

    IPC分类号： A61K47/48 ,  C08F289/00 ,  C08H1/00 ,  A61K38/48

    CPC分类号： A61K47/48176 ,  A61K38/4826 ,  A61K47/48769 ,  A61K47/58 ,  A61K47/69 ,  C08F289/00 ,  C08H1/00 ,  C12Y304/21001

    摘要： Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.

    摘要翻译： 使用新的具有水溶性的含活性酯酰胺的官能化受控自由基聚合引发剂，已经从蛋白质的表面生长了刺激响应性聚合物，例如糜蛋白酶或具有共价结合活性酯酰胺的表面氨基酸的任何蛋白质 的官能化引发剂。 显示温度或pH的变化可以改变酶周围的聚合物的构象，这反过来使得酶活性和稳定性的合理调整。 这种方法使酶的活性和稳定性提高了一个数量级的pH值，其中酶通常是无活性的或不稳定的。 描述了多峰温度反应性蛋白质 - 嵌段共聚物缀合物。

    POLYMER-BASED PROTEIN ENGINEERING METHODS TO RATIONALLY TUNE ENZYME ACTIVITY, pH-DEPENDENCE AND STABILITY
    3.
    发明申请
    POLYMER-BASED PROTEIN ENGINEERING METHODS TO RATIONALLY TUNE ENZYME ACTIVITY, pH-DEPENDENCE AND STABILITY 有权
    基于聚合物的蛋白质工程方法来显着调节酶活性，pH依赖性和稳定性

    公开(公告)号：US20160101190A1

    公开(公告)日：2016-04-14

    申请号：US14785868

    申请日：2014-04-22

    申请人： Alan J. Russell ,  Richard R. Koepsel ,  Chad Cummings ,  Hironobu Murata

    发明人： Alan J. Russell ,  Richard R. Koepsel ,  Chad Cummings ,  Hironobu Murata

    IPC分类号： A61K47/48 ,  A61K38/48

    CPC分类号： A61K47/48176 ,  A61K38/4826 ,  A61K47/48769 ,  A61K47/58 ,  A61K47/69 ,  C08F289/00 ,  C08H1/00 ,  C12Y304/21001

    摘要： Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.

    摘要翻译： 使用新的具有水溶性的含活性酯酰胺的官能化受控自由基聚合引发剂，已经从蛋白质的表面生长了刺激响应性聚合物，例如糜蛋白酶或具有共价结合活性酯酰胺的表面氨基酸的任何蛋白质 的官能化引发剂。 显示温度或pH的变化可以改变酶周围的聚合物的构象，这反过来使得酶活性和稳定性的合理调整。 这种方法使酶的活性和稳定性提高了一个数量级的pH值，其中酶通常是无活性的或不稳定的。 描述了多峰温度反应性蛋白质 - 嵌段共聚物缀合物。

    Manipulation of oxygen tension during in vitro follicle culture
    4.
    发明申请
    Manipulation of oxygen tension during in vitro follicle culture 审中-公开
    在体外卵泡培养中操纵氧气张力

    公开(公告)号：US20070207542A1

    公开(公告)日：2007-09-06

    申请号：US11713201

    申请日：2007-03-02

    申请人： Alan J. Russell ,  Matthew K. Heise ,  Richard R. Koepsel ,  Elizabeth A. McGee

    发明人： Alan J. Russell ,  Matthew K. Heise ,  Richard R. Koepsel ,  Elizabeth A. McGee

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0609 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2501/01 ,  C12N2517/10

    摘要： A process for culturing an immature ovarian follicle in vitro is disclosed. The process includes providing a culture having one or more immature ovarian follicles and providing a sufficient amount of oxygen to the ovarian culture on an intermittent basis, stepwise basis, or continuously, or combinations thereof, for a sufficient amount of time over the duration of the culture period to effect the maturation of the immature ovarian follicle. Over the course of the culture period, the oxygen concentration is raised until, at the end of the culture period, the oxygen concentration is in the range preferably between about 3% by volume (22.8 mmHg) and about 20% by volume (152 mmHg).

    摘要翻译： 公开了一种体外培养未成熟卵巢卵泡的方法。 该方法包括提供具有一个或多个未成熟卵巢卵泡的培养物，并且在间歇的基础上，逐步的基础上，或连续的或其组合在卵巢培养物中提供足够量的氧持续足够的时间 培养期可影响未成熟卵巢卵泡的成熟。 在培养期间，氧浓度升高，直到培养期结束时，氧浓度范围优选为约3体积％（22.8mmHg）至约20体积％（152mmHg） ）。