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    Myogenic vector systems
    6.
    发明授权
    Myogenic vector systems 失效
    造丝载体系统

    公开(公告)号：US5298422A

    公开(公告)日：1994-03-29

    申请号：US789919

    申请日：1991-11-06

    申请人： Robert J. Schwartz ,  Franco J. DeMayo ,  Bert W. O'Malley

    发明人： Robert J. Schwartz ,  Franco J. DeMayo ,  Bert W. O'Malley

    IPC分类号： A61K31/70 ,  A61K38/00 ,  A61K38/04 ,  A61K38/27 ,  A61K39/00 ,  A61K48/00 ,  A61P43/00 ,  C07H21/00 ,  C07K14/47 ,  C12N15/85 ,  C12N15/63

    CPC分类号： C12N15/85 ,  A61K48/00 ,  C07K14/4708 ,  A61K38/00 ,  A61K39/00 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/90

    摘要： The present invention includes a Myogenic vector system (MVS) for the expression of a nucleic acid sequence in myogenic tissue. The MVS contains a promoter, a cassette with the sequence of interest, a 3' untranslated region (3' UTR) and contiguous noncoding region (NCR). Further enhancements can include the addition of a leader sequence, intron sequence, initiation codon and specificThis invention was partially supported by a grant from the United States government under HL-38401 awarded by the National Institute of Health. The Government has certain rights in the invention.

    摘要翻译： 本发明包括用于在肌原性组织中表达核酸序列的致瘤载体系统（MVS）。 MVS包含启动子，具有感兴趣序列的盒，3'非翻译区（3'UTR）和连续非编码区（NCR）。 进一步的增强可以包括添加前导序列，内含子序列，起始密码子和特异性限制性内切核酸酶。 为了促进摄取和肌原性表达，MVS可以用组蛋白和由血清反应因子，转录起始因子和通过与血清反应元件和TATA盒相互作用连接到启动子的跨调节因子组成的DNA启动复合物包被。 MVS可以用于各种目的，包括基因替换，疫苗生产，疫苗接种，疾病治疗和肌肉萎缩。 当MVS被注入肌成纤维系统时，它将从感兴趣的序列产生多肽。

    COMPOSITION AND METHOD TO ALTER LEAN BODY MASS AND BONE PROPERTIES IN A SUBJECT
    8.
    发明申请
    COMPOSITION AND METHOD TO ALTER LEAN BODY MASS AND BONE PROPERTIES IN A SUBJECT 审中-公开
    组合物和方法，以提高身体质量和骨质量在一个主题

    公开(公告)号：US20090292107A1

    公开(公告)日：2009-11-26

    申请号：US12018582

    申请日：2008-01-23

    申请人： Ruxandra Draghia-Akli ,  Robert J. Schwartz

    发明人： Ruxandra Draghia-Akli ,  Robert J. Schwartz

    IPC分类号： C07K14/00

    CPC分类号： C07K14/60 ,  A61K38/25 ,  A61K48/00 ,  A61K48/0041 ,  A61K48/005 ,  A61K48/0083

    摘要： The present invention pertains to a method for decreasing the body fat proportion, increasing lean body mass (“LBM”), increasing bone density, or improving the rate of bone healing, or all, of a subject. Overall, the embodiments of the invention can be accomplished by delivering a heterologous nucleic acid sequence encoding GHRH or functional biological equivalent thereof into the cells of the subject and allowing expression of the encoded gene to occur while the modified cells are within the subject. For instance, when such a nucleic acid sequence is delivered into the specific cells of the subject tissue specific constitutive expression is achieved. Furthermore, external regulation of the GHRH or functional biological equivalent thereof gene can be accomplished by utilizing inducible promoters that are regulated by molecular switch molecules, which are given to the subject. The preferred method to deliver the constitutive or inducible nucleic acid encoding sequences of GHRH or the functional biological equivalents thereof is directly into the cells of the subject by the process of in vivo electroporation. A decrease the body fat proportion and an increase in lean body mass (“LBM”), or both of a subject is achieved by the delivery of GHRH or functional biological equivalent thereof as described herein by into the subject as recombinant proteins. In addition, an increase in bone density and improvement in the rate of bone healing is also achieved.

    摘要翻译： 本发明涉及一种降低身体脂肪比例，增加瘦体重（“LBM”），增加骨密度或改善受试者的骨愈合速率或全身的方法。 总的来说，本发明的实施方案可以通过将编码GHRH的异源核酸序列或其功能生物学等同物递送到受试者的细胞中并且允许在修饰的细胞在受试者内发生编码基因的表达来实现。 例如，当这样的核酸序列被递送到对象组织的特定细胞中时，实现了特异性组成型表达。 此外，GHRH或其功能生物等效基因的外部调节可以通过利用被给予受试者的由分子开关分子调节的诱导型启动子来实现。 递送GHRH的组成型或诱导型核酸编码序列或其功能生物学等价物的优选方法通过体内电穿孔的方法直接进入受试者的细胞。 通过将本文所述的GHRH或其功能生物学等同物作为重组蛋白递送到受试者中，可以降低身体脂肪比例和瘦体重增加（“LBM”）或两者。 此外，还可以实现骨密度的增加和骨愈合速度的提高。

    Polynucleotides encoding a super-active porcine growth hormone releasing hormone analog
    10.
    发明授权
    Polynucleotides encoding a super-active porcine growth hormone releasing hormone analog 有权
    编码超活性猪生长激素释放激素类似物的多核苷酸

    公开(公告)号：US07166461B2

    公开(公告)日：2007-01-23

    申请号：US10262141

    申请日：2002-10-01

    申请人： Robert J. Schwartz ,  Ruxandra Draghia-Alki

    发明人： Robert J. Schwartz ,  Ruxandra Draghia-Alki

    IPC分类号： C12N15/63 ,  C12N15/18

    CPC分类号： C07K14/60 ,  A61K38/00 ,  A61K48/00

    摘要： Inadequate growth due to deficiencies in growth hormone (GR), growth hormone releasing hormone (GHRH), or genetic diseases can be ameliorated utilizing recombinant protein therapy with a novel GHRH analog having a sequence (SEQ ID NO:1). Also included is (1) a method of treating growth hormone-related deficiencies associated with the growth hormone pathway; (2) a method for treating growth hormone-related deficiencies associated with genetic disease; (3) a method to improve growth performance in an animal; (4) a method of treating an animal having a growth deficiency disease; (5) a method of increasing the efficiency of an animal used for food; and (6) a method to enhance growth in an animal.

    摘要翻译： 可以利用具有序列（SEQ ID NO：1）的新型GHRH类似物利用重组蛋白质治疗来改善由于生长激素（GR），生长激素释放激素（GHRH）或遗传性疾病的缺陷导致的生长不足。 还包括（1）治疗与生长激素途径相关的生长激素相关缺陷的方法; （2）治疗与遗传性疾病相关的生长激素相关缺陷的方法; （3）改善动物生长性能的方法; （4）治疗具有生长缺陷病的动物的方法; （5）提高用于食物的动物的效率的方法; 和（6）增强动物生长的方法。