LAMININ-ENTACTIN COMPLEX AND CELL CULTURE ARTICLE AND METHODS THEREOF
    1.
    发明申请
    LAMININ-ENTACTIN COMPLEX AND CELL CULTURE ARTICLE AND METHODS THEREOF 有权
    LAMININ-ENTACTIN复合物和细胞培养物品及其方法

    公开(公告)号:US20110306136A1

    公开(公告)日:2011-12-15

    申请号:US13111234

    申请日:2011-05-19

    CPC classification number: C12N5/0068 C12M25/00 C12N2533/50 C12N2533/52

    Abstract: A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.

    Abstract translation: 一种细胞培养系统,包括:底物,底物涂层,一个或多个活细胞和覆盖源。 底物涂层和覆盖层可分别为层粘连蛋白和层粘连蛋白•内酰胺酶复合物。 或者,基底涂层和覆盖层可以分别是层粘连蛋白·内含子复合物和层粘连蛋白。 细胞培养系统还可以包括液体介质和保护盖。 还公开了在本文定义的细胞培养物和培养方法中制备和使用该系统的方法。

    Patterning Hydrogels
    2.
    发明申请
    Patterning Hydrogels 审中-公开
    图案水凝胶

    公开(公告)号:US20110053270A1

    公开(公告)日:2011-03-03

    申请号:US12862967

    申请日:2010-08-25

    Abstract: A method for forming a pattern-coated substrate includes disposing a composition comprising a polysaccharide-based polymer on a substrate to generate a coated substrate. The polysaccharide-based polymer composition is substantially free of cross-linking monomers. The method further includes exposing a portion of the coated substrate to a first dose of UV radiation to induce crosslinking of the polysaccharide-based polymer, wherein a portion of the substrate is shielded from the ionizing radiation. The UV exposed coated substrate may be washed or hydrated to remove uncross-linked polysaccharide-based polymer.

    Abstract translation: 形成图案涂布基板的方法包括在基材上设置包含多糖类聚合物的组合物以产生涂布的基材。 多糖类聚合物组合物基本上不含交联单体。 该方法还包括将涂覆的基底的一部分暴露于第一剂量的UV辐射以诱导多糖类聚合物的交联,其中基底的一部分与电离辐射屏蔽。 紫外线曝光的涂覆的基底可以被洗涤或水合以除去未交联的多糖基聚合物。

    Laminin-entactin complex and cell culture article and methods thereof
    3.
    发明授权
    Laminin-entactin complex and cell culture article and methods thereof 有权
    层粘蛋白 - 内含蛋白复合物和细胞培养物及其方法

    公开(公告)号:US08513015B2

    公开(公告)日:2013-08-20

    申请号:US13111234

    申请日:2011-05-19

    CPC classification number: C12N5/0068 C12M25/00 C12N2533/50 C12N2533/52

    Abstract: A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.

    Abstract translation: 一种细胞培养系统,包括:底物,底物涂层,一个或多个活细胞和覆盖源。 基底涂层和覆盖层可分别为层粘连蛋白和层粘连蛋白复合物。 或者,基底涂层和覆盖层可以分别是层粘连蛋白复合物和层粘连蛋白。 细胞培养系统还可以包括液体介质和保护盖。 还公开了在本文定义的细胞培养物和培养方法中制备和使用该系统的方法。

    Surface nanoreplication using polymer nanomasks
    6.
    发明授权
    Surface nanoreplication using polymer nanomasks 有权
    使用聚合物纳米掩模的表面纳米应用

    公开(公告)号:US09566609B2

    公开(公告)日:2017-02-14

    申请号:US13748827

    申请日:2013-01-24

    CPC classification number: B05D5/00 B82Y10/00 B82Y40/00 G03F7/0002

    Abstract: Methods for replicating a nanopillared surface include applying a nanopillar-forming material to a surface of a replica substrate to form a precursor layer on the replica-substrate surface. A template surface of a nanomask may be contacted to the precursor layer. The nanomask may include a self-assembled polymer layer on a nanomask-substrate surface, the template surface being defined in the self-assembled polymer layer. The self-assembled polymer layer may have nano-sized pores with openings at the template surface. The precursor layer may be cured while the template surface remains in contact with the precursor layer. The nanomask is removed to expose a nanopillared surface having a plurality of nanopillars on the replica-substrate surface. The nanopillars on the replica-substrate surface may correspond to the pores in the template surface. Nanopillared surfaces may be replicated on one side of the replica substrate or on two opposing sides of the replica substrate.

    Abstract translation: 用于复制纳米孔径表面的方法包括将纳米柱形成材料施加到复制基底的表面以在复制基底表面上形成前体层。 纳米掩模的模板表面可以与前体层接触。 纳米掩模可以包括在纳米掩模 - 衬底表面上的自组装聚合物层,模板表面限定在自组装聚合物层中。 自组装聚合物层可以具有在模板表面处具有开口的纳米尺寸的孔。 当模板表面保持与前体层接触时,前体层可以被固化。 去除纳米掩模以暴露在复制衬底表面上具有多个纳米柱的纳米圆柱形表面。 复制衬底表面上的纳米柱可以对应于模板表面中的孔。 纳米球形表面可以在复制基底的一侧或复制基底的两个相对侧上复制。

    SURFACE NANOREPLICATION USING POLYMER NANOMASKS
    7.
    发明申请
    SURFACE NANOREPLICATION USING POLYMER NANOMASKS 有权
    使用聚合物纳米颗粒的表面纳米粒子

    公开(公告)号:US20140205766A1

    公开(公告)日:2014-07-24

    申请号:US13748827

    申请日:2013-01-24

    CPC classification number: B05D5/00 B82Y10/00 B82Y40/00 G03F7/0002

    Abstract: Methods for replicating a nanopillared surface include applying a nanopillar-forming material to a surface of a replica substrate to form a precursor layer on the replica-substrate surface. A template surface of a nanomask may be contacted to the precursor layer. The nanomask may include a self-assembled polymer layer on a nanomask-substrate surface, the template surface being defined in the self-assembled polymer layer. The self-assembled polymer layer may have nano-sized pores with openings at the template surface. The precursor layer may be cured while the template surface remains in contact with the precursor layer. The nanomask is removed to expose a nanopillared surface having a plurality of nanopillars on the replica-substrate surface. The nanopillars on the replica-substrate surface may correspond to the pores in the template surface. Nanopillared surfaces may be replicated on one side of the replica substrate or on two opposing sides of the replica substrate.

    Abstract translation: 用于复制纳米孔径表面的方法包括将纳米柱形成材料施加到复制基底的表面以在复制基底表面上形成前体层。 纳米掩模的模板表面可以与前体层接触。 纳米掩模可以包括在纳米掩模 - 衬底表面上的自组装聚合物层,模板表面限定在自组装聚合物层中。 自组装聚合物层可以具有在模板表面处具有开口的纳米尺寸的孔。 当模板表面保持与前体层接触时,前体层可以被固化。 去除纳米掩模以暴露在复制衬底表面上具有多个纳米柱的纳米圆柱形表面。 复制衬底表面上的纳米柱可以对应于模板表面中的孔。 纳米球形表面可以在复制基底的一侧或复制基底的两个相对侧上复制。

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