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    Method for assessing biofilms
    3.
    发明申请
    Method for assessing biofilms 审中-公开
    生物膜评估方法

    公开(公告)号：US20060275847A1

    公开(公告)日：2006-12-07

    申请号：US10514138

    申请日：2003-04-28

    Applicant: Ian Goodyer ,  Rudi Labarbe ,  Dietrich Ruehlmann ,  Simon Stubbs

    Inventor： Ian Goodyer ,  Rudi Labarbe ,  Dietrich Ruehlmann ,  Simon Stubbs

    IPC: G01N33/554 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/26 ,  C12Q1/04

    CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/245

    Abstract: An automated method for measuring the development of a biofilm, containing one or more fluorescent moieties, on a plurality of surfaces using a confocal imaging system including: a) a radiation source system for forming a beam of electromagnetic radiation including one or more wavelengths; b) an optical system for directing and focusing said beam onto one or more planes of the object; c) a detection system for detecting electromagnetic radiation emitted from the object and producing image data; and d) a scanning system for scanning the object in a plurality of planes with the electromagnetic radiation, the method comprising the steps of: i) growing said biofilm on said plurality of surfaces; ii) detecting the presence of said one or more fluorescent moieties within the biofilm by scanning the biofilm with electromagnetic radiation in a plurality of planes and collecting fluorescent emissions to produce a plurality of images; and iii) analysing said images by means of a data processing system under the control of computer software to determine the structure of the biofilm.

    Abstract translation: 一种用于使用共焦成像系统在多个表面上测量含有一个或多个荧光部分的生物膜的开发的自动化方法，所述共聚焦成像系统包括：a）用于形成包括一个或多个波长的电磁辐射束的辐射源系统; b）用于将所述光束引导和聚焦到所述物体的一个或多个平面上的光学系统; c）用于检测从物体发射的电磁辐射并产生图像数据的检测系统; 以及d）用于利用所述电磁辐射在多个平面中扫描所述物体的扫描系统，所述方法包括以下步骤：i）在所述多个表面上生长所述生物膜; ii）通过在多个平面中用电磁辐射扫描生物膜并且收集荧光发射以产生多个图像来检测生物膜内所述一个或多个荧光部分的存在; 以及iii）在计算机软件的控制下通过数据处理系统分析所述图像，以确定生物膜的结构。

    Cell cycle reporting cell line
    5.
    发明授权
    Cell cycle reporting cell line 有权
    细胞周期报告细胞系

    公开(公告)号：US07745123B2

    公开(公告)日：2010-06-29

    申请号：US11572516

    申请日：2005-07-22

    Applicant: Suzanne Hancock ,  Simon Stubbs ,  Nicholas Thomas ,  Ellen Fanning ,  Jinming Gu

    Inventor： Suzanne Hancock ,  Simon Stubbs ,  Nicholas Thomas ,  Ellen Fanning ,  Jinming Gu

    IPC: C12Q1/68

    CPC classification number: G01N33/5011

    Abstract: The present invention relates to non-destructive and dynamic means for determining the cell cycle position of living cells. The invention provides stable cell lines which can be used to determine the cell cycle position, together with methods for measuring the effect of a test agent on the cell cycle position.

    Abstract translation: 本发明涉及用于确定活细胞的细胞周期位置的非破坏性和动态方法。 本发明提供可用于确定细胞周期位置的稳定细胞系，以及测量试剂对细胞周期位置的影响的方法。

    APPARATUS AND METHOD FOR DETECTING DNA DAMAGE
    7.
    发明申请
    APPARATUS AND METHOD FOR DETECTING DNA DAMAGE 审中-公开
    检测DNA损伤的装置和方法

    公开(公告)号：US20120058467A1

    公开(公告)日：2012-03-08

    申请号：US12746169

    申请日：2008-12-05

    Applicant: Nicholas Thomas ,  Michael Kenrick ,  Simon Stubbs

    Inventor： Nicholas Thomas ,  Michael Kenrick ,  Simon Stubbs

    IPC: C12Q1/68 ,  C12M1/34

    CPC classification number: G01N27/3275 ,  B01L3/5085 ,  B01L2300/046 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2400/0421 ,  G01N27/44773

    Abstract: The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage. The invention is particularly suited for use in Comet assays and for automation of such assays. The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein; and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply.

    Abstract translation: 本发明涉及用于检测细胞DNA损伤的装置和原位方法。 本发明特别适用于彗星测定和这种测定的自动化。 该设备包括多孔板，该板包括彼此保持固定关系的孔阵列，每个孔具有轴线，侧壁和基底，并且其中设置有设置在其中的电极对; 并且其中提供了用于将电极对并联连接到外部电压源的装置。

    Fluorescent proteins
    10.
    发明申请
    Fluorescent proteins 有权

    公开(公告)号：US20060036078A1

    公开(公告)日：2006-02-16

    申请号：US11251209

    申请日：2005-10-14

    Applicant: Simon Stubbs ,  Anne Jones ,  Nigel Michael ,  Nicholas Thomas

    Inventor： Simon Stubbs ,  Anne Jones ,  Nigel Michael ,  Nicholas Thomas

    IPC: C07K14/435 ,  C07H21/04 ,  C12P21/06

    CPC classification number: C07K14/43595 ,  C07K2319/00

    Abstract: The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.

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