公开(公告)号：US08367367B2

公开(公告)日：2013-02-05

申请号：US13155944

申请日：2011-06-08

申请人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

发明人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

IPC分类号： C12Q1/26

CPC分类号： G01N33/5079 ,  G01N2333/90209

摘要： A method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles having competent mitochondrial enzymes formed from inner membranes of mitochondria, an electron donor which transmits electrons to an electron transfer system of the sub-mitochondrial particles and the sample, and forming reaction resultants, adjusting a pH of a pH indicator which change color according to a change in pH, adding the pH indicators to each reaction resultant and identifying color changes of the pH indicator. Also provided is a kit for positively detecting toxic materials within a sample.

摘要翻译： 一种积极检测样品内的有毒物质的方法，该方法包括使具有由线粒体内膜形成的感受态线粒体酶的亚线粒体颗粒，将电子传递到亚线粒体颗粒的电子转移系统的电子给体和样品 ，并形成反应产物，调节pH指示剂的pH值，根据pH的变化改变颜色，向每个反应产物添加pH指示剂并鉴定pH指示剂的颜色变化。 还提供了用于积极检测样品中有毒物质的试剂盒。

公开(公告)号：US20100231308A1

公开(公告)日：2010-09-16

申请号：US12404595

申请日：2009-03-16

申请人： Hun Joo LEE ,  Soo Suk LEE ,  Soo Hyung CHOI

发明人： Hun Joo LEE ,  Soo Suk LEE ,  Soo Hyung CHOI

IPC分类号： G01N27/00 ,  H03H9/25 ,  H02N2/00

CPC分类号： G01N29/022 ,  G01N29/348 ,  G01N2291/0256 ,  G01N2291/0423

摘要： A surface acoustic wave (“SAW”) sensor includes; a first signal generator which generates a first signal having a predetermined frequency bandwidth using a pseudo random sequence, a second signal generator which generates a second signal with a predetermined frequency, a signal blender which blends the first signal with the second signal to generate a blended signal having the predetermined frequency bandwidth with the predetermined frequency as a center frequency, a wave generator which generates a surface acoustic wave using the blended signal, which converts the surface acoustic wave into a third signal after the surface acoustic wave travels a predetermined distance, and which outputs the third signal, and a signal detector which detects a change in the third signal from the wave generator to sense a substance bound to the wave generator.

摘要翻译： 声表面波（“SAW”）传感器包括： 使用伪随机序列产生具有预定频率带宽的第一信号的第一信号发生器，产生具有预定频率的第二信号的第二信号发生器，将第一信号与第二信号混合以产生混合的信号混合器 具有预定频率的预定频率的信号作为中心频率;波形发生器，其使用所述混合信号产生表面声波，其在表面声波行进预定距离之后将表面声波转换为第三信号;以及 其输出第三信号，以及信号检测器，其检测来自波发生器的第三信号的变化，以检测与波发生器绑定的物质。

公开(公告)号：US20090064859A1

公开(公告)日：2009-03-12

申请号：US12056376

申请日：2008-03-27

申请人： Hun Joo Lee ,  Soo Suk Lee ,  Soo Hyung Choi

发明人： Hun Joo Lee ,  Soo Suk Lee ,  Soo Hyung Choi

IPC分类号： B01D45/00 ,  B01D19/00

CPC分类号： B01D19/0042

摘要： A 3-dimensional air bubble trapping apparatus includes a plurality of chambers, each having an inflow and outflow channel at both ends, and which traps air bubbles in a material introduced through the inflow channel, wherein the chambers are divided into a previous chamber and a next chamber based on a moving direction of the material in the chamber, and wherein the outflow channel of the previous chamber is connected to the inflow channel of the next chamber, and wherein a face perpendicular to an outflow direction of the material in the outflow channel of the previous chamber is not disposed parallel with a face perpendicular to the outflow direction of the material in the outflow channel of the next chamber, whereby air bubbles in the material are trapped without being affected by an angle defined by a gravity direction and vibration of the apparatus.

摘要翻译： 三维气泡捕集装置包括多个室，每个室在两端具有流入和流出通道，并且在通过流入通道引入的材料中捕获气泡，其中所述室被分为先前的室和 基于所述室中的材料的移动方向的下一个室，并且其中所述先前室的所述流出通道连接到所述下一个室的流入通道，​​并且其中垂直于所述流出通道中的材料的流出方向的面 前一个室的垂直于下一个室的流出通道中的材料的流出方向的表面不平行设置，由此材料中的气泡被捕获而不受由重力方向和振动限定的角度的影响 该装置。

公开(公告)号：US20080135101A1

公开(公告)日：2008-06-12

申请号：US11869835

申请日：2007-10-10

申请人： Hun-joo LEE ,  Nam HUH ,  Soo-suk LEE ,  Sung-ouk JUNG ,  Soo-hyung CHOI

发明人： Hun-joo LEE ,  Nam HUH ,  Soo-suk LEE ,  Sung-ouk JUNG ,  Soo-hyung CHOI

IPC分类号： B81B1/00 ,  B21D51/16 ,  G05D7/03

CPC分类号： G05D7/0694 ,  Y10T29/494 ,  Y10T137/0753 ,  Y10T137/2191 ,  Y10T137/86187

摘要： The microfluidic device includes a rotatable platform, a plurality of connection ports disposed at a portion of the platform proximate to a shaft connection hole, the plurality of connection ports capable of being connected to an external connector for selectively injecting and discharging fluid and being closed by the connector, a trap chamber disposed at a portion of the platform further away from the shaft connection hole than the plurality of connection ports, the trap chamber including an inlet connected with at least one connection port of the plurality of connection ports, an outlet connected with another connection port of the plurality of connection ports and structures which enlarge a contact area with the fluid and a temporary storage including an inlet connected with the outlet of the trap chamber and an outlet connected with another connection port of the plurality of connection ports.

摘要翻译： 所述微流体装置包括可旋转平台，多个连接端口，设置在所述平台的靠近轴连接孔的部分处，所述多个连接端口能够连接到外部连接器，用于选择性地注入和排出流体并且由 所述连接器，设置在所述平台的远离所述轴连接孔的所述平台部分处的所述收集室，所述收集室包括与所述多个连接端口中的至少一个连接端口连接的入口，所述入口连接到所述入口 多个连接端口中的另一个连接端口和扩大与流体的接触面积的结构以及包括与捕集室的出口连接的入口的临时存储器和与多个连接端口中的另一连接端口连接的出口。

公开(公告)号：US08047077B2

公开(公告)日：2011-11-01

申请号：US12404595

申请日：2009-03-16

申请人： Hun Joo Lee ,  Soo Suk Lee ,  Soo Hyung Choi

发明人： Hun Joo Lee ,  Soo Suk Lee ,  Soo Hyung Choi

IPC分类号： G01N29/36 ,  H02N2/00

CPC分类号： G01N29/022 ,  G01N29/348 ,  G01N2291/0256 ,  G01N2291/0423

摘要： A surface acoustic wave (“SAW”) sensor includes; a first signal generator which generates a first signal having a predetermined frequency bandwidth using a pseudo random sequence, a second signal generator which generates a second signal with a predetermined frequency, a signal blender which blends the first signal with the second signal to generate a blended signal having the predetermined frequency bandwidth with the predetermined frequency as a center frequency, a wave generator which generates a surface acoustic wave using the blended signal, which converts the surface acoustic wave into a third signal after the surface acoustic wave travels a predetermined distance, and which outputs the third signal, and a signal detector which detects a change in the third signal from the wave generator to sense a substance bound to the wave generator.

摘要翻译： 声表面波（“SAW”）传感器包括： 使用伪随机序列产生具有预定频率带宽的第一信号的第一信号发生器，产生具有预定频率的第二信号的第二信号发生器，将第一信号与第二信号混合以产生混合的信号混合器 具有预定频率的预定频率的信号作为中心频率;波形发生器，其使用所述混合信号产生表面声波，其在表面声波行进预定距离之后将表面声波转换为第三信号;以及 其输出第三信号，以及信号检测器，其检测来自波发生器的第三信号的变化，以检测与波发生器绑定的物质。

公开(公告)号：US07662614B2

公开(公告)日：2010-02-16

申请号：US11330566

申请日：2006-01-11

申请人： Jang-seok Ma ,  Heon-su Jeon ,  Soo-hyung Choi ,  In-ho Lee ,  Fu Wang

发明人： Jang-seok Ma ,  Heon-su Jeon ,  Soo-hyung Choi ,  In-ho Lee ,  Fu Wang

IPC分类号： C12M1/34

CPC分类号： G01N21/6428 ,  B82Y20/00

摘要： Provided are a biochip platform for biochemically analyzing a sample such as DNA or protein, including a dielectric particle layer, and an optical assay apparatus including the same. The biochip platform includes the dielectric particle layer uniformly formed on a substrate. The particle uniformity of the dielectric particle layer enables good wavelength separation of fluorescence signal, and the large surface area of the dielectric particle layer guarantees better amplifications efficiency of fluorescence signal. Furthermore, the biochip platform shows good economical efficiency due to easy fabrication process, and is particularly useful in an optical assay apparatus for analyzing a biochemical sample due to good assay efficiency.

摘要翻译： 提供了用于生物化学分析诸如DNA或蛋白质的样品（包括电介质颗粒层）的生物芯片平台和包括其的光学测定装置。 生物芯片平台包括均匀地形成在基板上的电介质颗粒层。 电介质颗粒层的粒子均匀性使得荧光信号的波长分离良好，并且电介质颗粒层的大的表面积保证了荧光信号的更好的放大效率。 此外，生物芯片平台由于易于制造工艺而显示出良好的经济效率，并且在由于良好的测定效率分析生物化学样品的光学测定装置中特别有用。

公开(公告)号：US20080142361A1

公开(公告)日：2008-06-19

申请号：US11745522

申请日：2007-05-08

申请人： Jung-im HAN ,  Soo-hyung CHOI ,  Jeong-hee LEE ,  Soo-suk LEE ,  Jeong-na HEO

发明人： Jung-im HAN ,  Soo-hyung CHOI ,  Jeong-hee LEE ,  Soo-suk LEE ,  Jeong-na HEO

IPC分类号： G01N27/26 ,  B05D3/00 ,  B05D5/00

CPC分类号： G01N27/127 ,  Y10S977/742 ,  Y10S977/743 ,  Y10S977/953 ,  Y10S977/957

摘要： A carbon nanotube (“CNT”) gas sensor includes a substrate, an insulating layer formed on the substrate, electrodes formed on the insulating layer, and CNT barriers that protrude higher than the electrodes in spaces between the electrodes to form gas detecting spaces. A method of manufacturing the gas sensor includes forming an insulating layer on a substrate, forming an electrode pattern on the insulating layer, coating CNT paste having a thickness greater than a thickness of electrodes in the electrode pattern on the electrodes and the insulating layer, and patterning and firing the carbon nanotube paste, including using a photolithography method, to retain only portions of the CNT paste coated on spaces between the electrodes.

摘要翻译： 碳纳米管（“CNT”）气体传感器包括基板，形成在基板上的绝缘层，形成在绝缘层上的电极和在电极之间的空间中比电极突出的CNT势垒，以形成气体检测空间。 制造气体传感器的方法包括在基板上形成绝缘层，在绝缘层上形成电极图案，在电极和绝缘层上涂覆厚度大于电极图案的电极厚度的CNT浆料，以及 包括使用光刻法构图和烧制碳纳米管糊，仅保留涂覆在电极之间的间隙上的部分CNT浆料。

公开(公告)号：US20070148723A1

公开(公告)日：2007-06-28

申请号：US11610851

申请日：2006-12-14

申请人： Jung-im HAN ,  Soo-hyung CHOI

发明人： Jung-im HAN ,  Soo-hyung CHOI

IPC分类号： C12Q1/26

CPC分类号： G01N33/5079 ,  G01N33/5035 ,  G01N2333/90209

摘要： Provided is a method for detecting the degree of toxicity of toxic materials within a sample. The method includes contacting sub-mitochondrial particles having competent mitochondrial enzyme formed from the inner membranes of mitochondria, an electron donor which transmits electrons to the electron transfer system of the sub-mitochondrial particles, and a sample which will be tested; adding tris-2,2′-bipyridyl-ruthenium (II) ion to the reaction mixture; and measuring electrical variables of the reaction mixture. Also, provided is an apparatus and kit for detecting the presence of toxic materials within a sample.

摘要翻译： 提供了用于检测样品中有毒物质的毒性程度的方法。 该方法包括使具有由线粒体内膜形成的具有感受态的线粒体酶的亚线粒体颗粒和将电子传递到亚线粒体颗粒的电子传递系统的电子供体以及待测试的样品; 向反应混合物中加入三 - 2,2'-联吡啶 - 钌（II）离子; 并测量反应混合物的电变量。 此外，提供了用于检测样品中有毒物质的存在的装置和试剂盒。

公开(公告)号：US07918924B2

公开(公告)日：2011-04-05

申请号：US12056376

申请日：2008-03-27

申请人： Hun Joo Lee ,  Soo Suk Lee ,  Soo Hyung Choi

发明人： Hun Joo Lee ,  Soo Suk Lee ,  Soo Hyung Choi

IPC分类号： B01D19/00

CPC分类号： B01D19/0042

摘要： A 3-dimensional air bubble trapping apparatus includes a plurality of chambers, each having an inflow and outflow channel at both ends, and which traps air bubbles in a material introduced through the inflow channel, wherein the chambers are divided into a previous chamber and a next chamber based on a moving direction of the material in the chamber, and wherein the outflow channel of the previous chamber is connected to the inflow channel of the next chamber, and wherein a face perpendicular to an outflow direction of the material in the outflow channel of the previous chamber is not disposed parallel with a face perpendicular to the outflow direction of the material in the outflow channel of the next chamber, whereby air bubbles in the material are trapped without being affected by an angle defined by a gravity direction and vibration of the apparatus.

摘要翻译： 三维气泡捕集装置包括多个室，每个室在两端具有流入和流出通道，并且在通过流入通道引入的材料中捕获气泡，其中所述室被分成先前的室和 基于所述室中的材料的移动方向的下一个室，并且其中所述先前室的所述流出通道连接到所述下一个室的流入通道，​​并且其中垂直于所述流出通道中的材料的流出方向的面 前一个室的垂直于下一个室的流出通道中的材料的流出方向的表面不平行设置，由此材料中的气泡被捕获而不受由重力方向和振动限定的角度的影响 该装置。

公开(公告)号：US07803540B2

公开(公告)日：2010-09-28

申请号：US11335229

申请日：2006-01-19

申请人： Soo-hyung Choi ,  Jang-seok Ma ,  Joon-shik Park ,  Ji-young Oh ,  Jong-suk Chung

发明人： Soo-hyung Choi ,  Jang-seok Ma ,  Joon-shik Park ,  Ji-young Oh ,  Jong-suk Chung

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

CPC分类号： C12Q1/6827 ,  C12Q2537/113 ,  C12Q2527/107 ,  C12Q2525/185

摘要： A method of increasing discrimination for a target DNA having a polymorphic site is provided. The method comprising immobilizing first and second probes on a substrate; hybridizing the immobilized first and second probes with first and second hurdle DNAs, respectively; and hybridizing the target DNA with the hybrids, and determining the ratio of a signal of the target DNA hybridized to the first probe to a signal of the target DNA hybridized to the second probe. The addition of a hurdle DNA and variation of a probe base can improve an ability of discriminating a single base mismatch.

摘要翻译： 提供了增加具有多态位点的靶DNA的鉴别方法。 所述方法包括将第一和第二探针固定在基底上; 分别将固定的第一和第二探针与第一和第二障碍DNA杂交; 并且将目标DNA与杂交体杂交，并确定与第一探针杂交的靶DNA的信号与与第二探针杂交的靶DNA的信号的比率。 障碍DNA的添加和探针碱基的变异可以提高鉴别单一碱基不匹配的能力。