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    Kits containing modified amplification oligonucleotides
    1.
    发明申请
    Kits containing modified amplification oligonucleotides 审中-公开
    试剂盒含有修饰的扩增寡核苷酸

    公开(公告)号：US20050106610A1

    公开(公告)日：2005-05-19

    申请号：US10973162

    申请日：2004-10-26

    申请人： Michael Becker ,  Mehrdad Majlessi ,  Steven Brentano

    发明人： Michael Becker ,  Mehrdad Majlessi ,  Steven Brentano

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/6832 ,  C12Q1/686 ,  C12Q1/6865 ,  C12P19/34

    CPC分类号： C07H21/00 ,  C12Q1/6832 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2527/107 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2537/101 ,  C12Q2565/107

    摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

    摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。

    METHODS AND KITS FOR AMPLIFYING DNA
    2.
    发明申请
    METHODS AND KITS FOR AMPLIFYING DNA 有权
    用于扩增DNA的方法和工具

    公开(公告)号：US20070202523A1

    公开(公告)日：2007-08-30

    申请号：US11681104

    申请日：2007-03-01

    申请人： Michael BECKER ,  Wai-Chung Lam ,  Kristin Livezey ,  Steven Brentano ,  Daniel Kolk ,  Astrid Schroder

    发明人： Michael BECKER ,  Wai-Chung Lam ,  Kristin Livezey ,  Steven Brentano ,  Daniel Kolk ,  Astrid Schroder

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12P19/34 ,  C12Q1/6865 ,  C12Q2533/101 ,  C12Q2525/186 ,  C12Q2525/143 ,  C12Q2537/163 ,  C12Q2521/325 ,  C12Q2521/107

    摘要： Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3′-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-pro ducts. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.

    摘要翻译： 公开了合成自动催化的靶核酸序列的多个拷贝的新方法（即，能够自动循环而不需要修改反应条件如温度，pH或离子强度，并且使用下一个循环的产物 一）。 特别地，公开了鲁棒和有效的核酸扩增方法，同时减少副产物的外观。 通常，所述方法使用仅靶向靶核酸的引物寡核苷酸，经修饰以防止聚合酶从其3'末端延伸的启动子寡核苷酸，以及任选的终止引物延伸反应的手段以扩增RNA或 DNA分子在体外，同时减少或基本上消除副产物的形成。 所公开的方法最小化或基本上消除副产物的出现，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 所公开的方法最小化或基本上消除了该问题，从而提供了增强的灵敏度水平。

    Detection rpoB sequences of Mycobacterium Tuberculosis
    3.
    发明申请
    Detection rpoB sequences of Mycobacterium Tuberculosis 有权
    检测结核分枝杆菌的rpoB序列

    公开(公告)号：US20060263823A1

    公开(公告)日：2006-11-23

    申请号：US11495262

    申请日：2006-07-27

    申请人： Markus Jucker ,  Steven Brentano ,  Francisco Delgado ,  Philippe Cleuziat

    发明人： Markus Jucker ,  Steven Brentano ,  Francisco Delgado ,  Philippe Cleuziat

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/689 ,  C12Q2600/156

    摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.

    摘要翻译： 一种检测存在于生物样品中的结核分枝杆菌的rpoB序列的方法，其包括在包括特异性公开的引物序列的核酸扩增混合物中体外扩增结核分枝杆菌rpoB序列的步骤，并通过使用提供的探针检测扩增的序列 公开了通过其与部分扩增核酸的特异性杂交的信息。 公开了用于在体外扩增和检测样品中结核分枝杆菌的rpoB序列的组合物。

    Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
    8.
    发明申请
    Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe 审中-公开
    通过使用核酸杂交开关探针检测分析物的组合物和方法

    公开(公告)号：US20060194240A1

    公开(公告)日：2006-08-31

    申请号：US11363848

    申请日：2006-02-28

    申请人： Lyle Arnold ,  Lizhong Dai ,  Steven Brentano ,  James Russell

    发明人： Lyle Arnold ,  Lizhong Dai ,  Steven Brentano ,  James Russell

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q2525/197 ,  C12Q2525/161 ,  C12Q2565/107 ,  C12Q2563/131 ,  C12Q2525/301

    摘要： Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form a first conformation in the absence of the analyte and to form a second conformation in the presence of the analyte, and a label associated with the probe that produces a signal that indicates the conformation of the probe. Methods are described for detecting an analyte that forms a specific binding pair with the binding partner attached to the hybridization switch probe, thereby changing the probe from a first to a second conformation that results in a detectable signal that indicates the presence of the analyte in the sample.

    摘要翻译： 描述了用于检测分析物与连接到核酸杂交开关探针的结合配偶体的结合的组合物，其包含第一和第二臂序列以及与两个臂序列至少部分互补的支持序列，允许探针在杂交条件下 在不存在分析物的情况下形成第一构象，并在分析物存在下形成第二构象，以及与探针相关联的标记，其产生指示探针构象的信号。 描述了用于检测与附着到杂交开关探针的结合配偶体形成特异性结合对的分析物的方法，从而将探针从第一至第二构象改变，从而导致可检测的信号，其指示分析物在 样品。

    METHOD FOR DETECTING A STRUCTURED TARGET
    9.
    发明申请
    METHOD FOR DETECTING A STRUCTURED TARGET 有权
    检测结构目标的方法

    公开(公告)号：US20080090247A1

    公开(公告)日：2008-04-17

    申请号：US11925209

    申请日：2007-10-26

    申请人： Michael BECKER ,  Mehrdad MAJLESSI ,  Steven Brentano

    发明人： Michael BECKER ,  Mehrdad MAJLESSI ,  Steven Brentano

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2563/131 ,  C12Q2563/113 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2527/107

    摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

    摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。

    AMPLIFICATION AND DETECTION METHOD
    10.
    发明申请
    AMPLIFICATION AND DETECTION METHOD 有权
    放大和检测方法

    公开(公告)号：US20080090246A1

    公开(公告)日：2008-04-17

    申请号：US11924910

    申请日：2007-10-26

    申请人： Michael BECKER ,  Mehrdad MAJLESSI ,  Steven Brentano

    发明人： Michael BECKER ,  Mehrdad MAJLESSI ,  Steven Brentano

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2563/131 ,  C12Q2563/113 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2527/107

    摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

    摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。