公开(公告)号：US08804121B2

公开(公告)日：2014-08-12

申请号：US13642012

申请日：2011-04-13

申请人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

发明人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  C12M41/46 ,  G01N21/45 ,  G01N21/4788 ,  G02B21/14 ,  G02B21/34

摘要： A cell observation device is provided with a reflection interference measurement light source 106, a quantitative phase measurement light source 105, a reflection interference detection camera 110 which images light emitted from the reflection interference measurement light source 106 and reflected from cells 101, to generate a reflection interference image, a quantitative phase detection camera 112 which images light emitted from the quantitative phase measurement light source 105 and transmitted by the cells 101, to generate a quantitative phase image, an image alignment unit 201 which matches a spatial position of the reflection interference image with a spatial position of the quantitative phase image, to implement alignment between the two images, a first extraction unit 204 which extracts a first parameter from the reflection interference image in alignment with the quantitative phase image, and a second extraction unit 205 which extracts a second parameter from the quantitative phase image in alignment with the reflection interference image.

摘要翻译： 电池观察装置设置有反射干涉测量光源106，定量相位测量光源105，反射干涉检测照相机110，其反射从反射干涉测量光源106发射并从单元101反射的光，以产生 反射干涉图像，定量相位检测摄像机112，其对从定量相位测量光源105发射并由单元101发送的光进行成像以产生定量相位图像;图像对准单元201，其匹配反射干涉的空间位置 具有定量相位图像的空间位置的图像，以实现两个图像之间的对准;第一提取单元204，其从反射干涉图像中提取与定量相位图像对准的第一参数;以及第二提取单元205，其提取 来自定量的第二个参数 与反射干涉图像对准的相位图像。

公开(公告)号：US10379334B2

公开(公告)日：2019-08-13

申请号：US14127669

申请日：2012-06-19

申请人： Takuji Kataoka ,  Masanori Matsubara

发明人： Takuji Kataoka ,  Masanori Matsubara

IPC分类号： H04N7/18 ,  G02B21/36 ,  G01N21/64 ,  G06T7/00

摘要： A light measurement device is a light measurement device for measuring light coming from a sample, and is provided with a moving-image acquisition part for acquiring moving image data, and an analysis processing part for performing analysis processing on moving image data. The analysis processing part includes: a luminance-value-data acquisition part for acquiring luminance value data indicating a chronological change in a luminance value; a luminance-value extraction part for extracting a peak value and a bottom value of the luminance value, from the luminance value data; and a pixel specifying part for calculating an evaluation value evaluating a state of a change in a luminance value on the basis of the peak value and the bottom value and specifying a target pixel that is to be analyzed from a plurality of pixels on the basis of a repeat state of the evaluation value.

公开(公告)号：US08941079B2

公开(公告)日：2015-01-27

申请号：US13146212

申请日：2010-01-26

申请人： Takuji Kataoka ,  Taira Ito

发明人： Takuji Kataoka ,  Taira Ito

IPC分类号： F21V9/16 ,  G01N21/64

CPC分类号： G01N21/6452

摘要： The present invention relates to a light irradiation apparatus and the like which can reduce background light noise from a plurality of wells provided on a microplate. The light irradiation apparatus comprises a microplate, a light guiding member, and a light source apparatus. The light guiding member includes a plurality of light emitting units provided on a main surface in correspondence with the plurality of wells. Each light emitting unit includes depressions each having an opening on the main surface. Measurement light from the light source apparatus is inputted from a side surface, refracted and reflected at the side surface of each depression, and then outputted from the opening of each depression.

摘要翻译： 本发明涉及一种光照射装置等，其能够降低来自设在微孔板上的多个孔的背景光噪声。 光照射装置包括微孔板，导光构件和光源装置。 导光构件包括与多个孔相对应地设置在主表面上的多个发光单元。 每个发光单元包括在主表面上具有开口的凹陷。 来自光源装置的测量光从侧面输入，在各凹部的侧面折射反射，然后从各凹部的开口输出。

公开(公告)号：US08873027B2

公开(公告)日：2014-10-28

申请号：US13642027

申请日：2011-04-13

申请人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

发明人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

IPC分类号： G01B9/02 ,  G06T7/00 ,  G01N15/14 ,  G02B21/14 ,  C12M1/34 ,  G01N21/45

CPC分类号： G01N21/45 ,  C12M41/46 ,  G01N15/1475 ,  G01N2021/451 ,  G02B21/14 ,  G06K9/0014 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A cell observation device is provided with a reflection interference shutter 106A which adjusts a light quantity of light emitted from a reflection interference measurement light source 106, a quantitative phase shutter 105A which adjusts a light quantity of light emitted from a quantitative phase measurement light source, a camera 110 which images reflected light from the reflection interference measurement light source 106 to generate a reflection interference image and which images transmitted light from a quantitative phase measurement light source 105 to generate a quantitative phase image, and a first extraction unit 204 and a second extraction unit 205 which extract first and second parameters from the reflection interference image generated by the camera 110. During generation of the reflection interference image, the quantitative phase shutter 105A blocks the light from the quantitative phase measurement light source 105. During generation of the quantitative phase image, the reflection interference shutter 106A blocks the light from the reflection interference measurement light source 106.

摘要翻译： 电池观察装置设置有反射干涉光圈106A，该反射干涉光圈106A调节从反射干涉测量光源106发射的光的光量，调节从定量相位测量光源发射的光的光量的定量相位快门105A， 对来自反射干涉测量光源106的反射光进行成像以产生反射干涉图像的摄像机110，以及来自定量相位测量光源105的透射光以产生定量相位图像的图像，以及第一提取单元204和第二 提取单元205，其从由相机110产生的反射干涉图像中提取第一和第二参数。在产生反射干涉图像期间，定量相位快门105A阻挡来自定量相位测量光源105的光。在定量 相位图像， 反射干涉光闸106A阻挡来自反射干涉测量光源106的光。

公开(公告)号：US20140152799A1

公开(公告)日：2014-06-05

申请号：US14127700

申请日：2012-06-19

申请人： Takuji Kataoka ,  Masanori Matsubara

发明人： Takuji Kataoka ,  Masanori Matsubara

IPC分类号： G02B21/36

CPC分类号： G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06K9/0014 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30072

摘要： The light measurement device is provided with a moving-image acquisition part and an analysis processing part. The analysis processing part includes: a luminance-value-data acquisition part for acquiring the luminance value data; a luminance-value extraction part for extracting a peak value and a bottom value of the luminance value, from the luminance value data; a pixel extraction part for extracting a target pixel configuring an image of a predetermined cell from a plurality of pixels, on the basis of the evaluation value. The pixel extraction part extracts, as the evaluation value, the target pixel on the basis of at least one of an amplitude of the luminance value obtained from a difference between the peak value and the bottom value and a change ratio of the luminance value obtained from a ratio of the peak value relative to the bottom value.

摘要翻译： 光测量装置设置有运动图像获取部分和分析处理部分。 分析处理部分包括：用于获取亮度值数据的亮度值数据获取部分; 亮度值提取部分，用于从亮度值数据中提取亮度值的峰值和底部值; 基于评估值，提取从多个像素中提取构成预定单元的图像的目标像素的像素提取部。 像素提取部分根据从峰值和底部值之间的差值获得的亮度值的幅度与从下列值获得的亮度值的变化率中的至少一个，提取目标像素作为评估值作为评估值： 峰值相对于底值的比率。

公开(公告)号：US20130288286A1

公开(公告)日：2013-10-31

申请号：US13642012

申请日：2011-04-13

申请人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

发明人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  C12M41/46 ,  G01N21/45 ,  G01N21/4788 ,  G02B21/14 ,  G02B21/34

摘要： A cell observation device is provided with a reflection interference measurement light source 106, a quantitative phase measurement light source 105, a reflection interference detection camera 110 which images light emitted from the reflection interference measurement light source 106 and reflected from cells 101, to generate a reflection interference image, a quantitative phase detection camera 112 which images light emitted from the quantitative phase measurement light source 105 and transmitted by the cells 101, to generate a quantitative phase image, an image alignment unit 201 which matches a spatial position of the reflection interference image with a spatial position of the quantitative phase image, to implement alignment between the two images, a first extraction unit 204 which extracts a first parameter from the reflection interference image in alignment with the quantitative phase image, and a second extraction unit 205 which extracts a second parameter from the quantitative phase image in alignment with the reflection interference image.

摘要翻译： 电池观察装置设置有反射干涉测量光源106，定量相位测量光源105，反射干涉检测照相机110，其反射从反射干涉测量光源106发射并从单元101反射的光，以产生 反射干涉图像，定量相位检测摄像机112，其对从定量相位测量光源105发射并由单元101发送的光进行成像以产生定量相位图像;图像对准单元201，其匹配反射干涉的空间位置 具有定量相位图像的空间位置的图像，以实现两个图像之间的对准;第一提取单元204，其从反射干涉图像中提取与定量相位图像对准的第一参数;以及第二提取单元205，其提取 来自定量的第二个参数 与反射干涉图像对准的相位图像。

公开(公告)号：US20130088719A1

公开(公告)日：2013-04-11

申请号：US13642066

申请日：2011-04-13

申请人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

发明人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

IPC分类号： G01N21/45

CPC分类号： G01N21/45 ,  C12Q1/02 ,  G01N21/253

摘要： A membrane potential change detection device 1 is provided with a reflection interference measurement light source 106, a holder 103 which holds a transparent member 102a on which cells 101 are mounted, a reflection interference detection camera 110 which images light emitted from the reflection interference measurement light source 106 and reflected from the cells 101 through the transparent member 102a, to generate a reflection interference image, and an analysis unit 202 which calculates a parameter dI about adhesion between the cells 101 and the transparent member 102a from the reflection interference image and detects a change of membrane potential of the cells 101 on the basis of a change of the parameter dI.

摘要翻译： 膜电位变化检测装置1设置有反射干涉测量光源106，保持安装有电池101的透明构件102a的保持件103，反射干涉检测照相机110对从反射干涉测量光 源106并且通过透明构件102a从单元101反射，以产生反射干涉图像;以及分析单元202，其从反射干涉图像计算关于单元101和透明构件102a之间的粘附的参数dI，并且检测 基于参数dI的变化来改变电池101的膜电位。

公开(公告)号：US10197782B2

公开(公告)日：2019-02-05

申请号：US14127700

申请日：2012-06-19

申请人： Takuji Kataoka ,  Masanori Matsubara

发明人： Takuji Kataoka ,  Masanori Matsubara

IPC分类号： H04N7/18 ,  G02B21/36 ,  G06T7/00 ,  G06K9/00 ,  G01N21/64

摘要： The light measurement device is provided with a moving-image acquisition part and an analysis processing part. The analysis processing part includes: a luminance-value-data acquisition part for acquiring the luminance value data; a luminance-value extraction part for extracting a peak value and a bottom value of the luminance value, from the luminance value data; a pixel extraction part for extracting a target pixel configuring an image of a predetermined cell from a plurality of pixels, on the basis of the evaluation value. The pixel extraction part extracts, as the evaluation value, the target pixel on the basis of at least one of an amplitude of the luminance value obtained from a difference between the peak value and the bottom value and a change ratio of the luminance value obtained from a ratio of the peak value relative to the bottom value.

公开(公告)号：US20140152798A1

公开(公告)日：2014-06-05

申请号：US14127669

申请日：2012-06-19

申请人： Takuji Kataoka ,  Masanori Matsubara

发明人： Takuji Kataoka ,  Masanori Matsubara

IPC分类号： G02B21/36

CPC分类号： G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30242

摘要： A light measurement device is a light measurement device for measuring light coming from a sample, and is provided with a moving-image acquisition part for acquiring moving image data, and an analysis processing part for performing analysis processing on moving image data. The analysis processing part includes: a luminance-value-data acquisition part for acquiring luminance value data indicating a chronological change in a luminance value; a luminance-value extraction part for extracting a peak value and a bottom value of the luminance value, from the luminance value data; and a pixel specifying part for calculating an evaluation value evaluating a state of a change in a luminance value on the basis of the peak value and the bottom value and specifying a target pixel that is to be analyzed from a plurality of pixels on the basis of a repeat state of the evaluation value.

摘要翻译： 光测量装置是用于测量来自样品的光的光测量装置，并且设置有用于获取运动图像数据的运动图像获取部分，以及用于对运动图像数据进行分析处理的分析处理部分。 分析处理部分包括：亮度值数据获取部分，用于获取指示亮度值的时间变化的亮度值数据; 亮度值提取部分，用于从亮度值数据中提取亮度值的峰值和底部值; 以及像素指定部分，用于基于峰值和底部值来计算评估亮度值的变化的状态的评估值，并且基于从多个像素指定要分析的目标像素 评估值的重复状态。

公开(公告)号：US20130130307A1

公开(公告)日：2013-05-23

申请号：US13642027

申请日：2011-04-13

申请人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

发明人： Norikazu Sugiyama ,  Takuji Kataoka ,  Takahiro Ikeda

IPC分类号： C12Q1/04

CPC分类号： G01N21/45 ,  C12M41/46 ,  G01N15/1475 ,  G01N2021/451 ,  G02B21/14 ,  G06K9/0014 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A cell observation device is provided with a reflection interference shutter 106A which adjusts a light quantity of light emitted from a reflection interference measurement light source 106, a quantitative phase shutter 105A which adjusts a light quantity of light emitted from a quantitative phase measurement light source, a camera 110 which images reflected light from the reflection interference measurement light source 106 to generate a reflection interference image and which images transmitted light from a quantitative phase measurement light source 105 to generate a quantitative phase image, and a first extraction unit 204 and a second extraction unit 205 which extract first and second parameters from the reflection interference image generated by the camera 110. During generation of the reflection interference image, the quantitative phase shutter 105A blocks the light from the quantitative phase measurement light source 105. During generation of the quantitative phase image, the reflection interference shutter 106A blocks the light from the reflection interference measurement light source 106.

摘要翻译： 电池观察装置设置有反射干涉光圈106A，该反射干涉光圈106A调节从反射干涉测量光源106发射的光的光量，调节从定量相位测量光源发射的光的光量的定量相位快门105A， 对来自反射干涉测量光源106的反射光进行成像以产生反射干涉图像的摄像机110，以及来自定量相位测量光源105的透射光以产生定量相位图像的图像，以及第一提取单元204和第二 提取单元205，其从由相机110产生的反射干涉图像中提取第一和第二参数。在产生反射干涉图像期间，定量相位快门105A阻挡来自定量相位测量光源105的光。在定量 相位图像， 反射干涉光闸106A阻挡来自反射干涉测量光源106的光。