-
公开(公告)号：US20080032408A1
公开(公告)日：2008-02-07
申请号：US11562548
申请日：2006-11-22
IPC分类号： G01N33/00
CPC分类号： C12Q1/6837 , Y10T436/13 , C12Q2525/161
摘要： The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.
摘要翻译： 本发明提供了一组独特的核酸序列，其适用于需要核酸标签的各种应用。 因此，本发明的序列标签可用于例如在基因分型应用中和各种其它分析中标记生物和非生物材料。
-
公开(公告)号：US07157564B1
公开(公告)日：2007-01-02
申请号：US09827383
申请日：2001-04-04
IPC分类号： C07H21/02
CPC分类号： C12Q1/6837 , Y10T436/13 , C12Q2525/161
摘要： The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.
摘要翻译： 本发明提供了一组独特的核酸序列，其适用于需要核酸标签的各种应用。 因此，本发明的序列标签可用于例如在基因分型应用中和各种其它分析中标记生物和非生物材料。
-
公开(公告)号：US06709816B1
公开(公告)日：2004-03-23
申请号：US09419817
申请日：1999-10-18
申请人： Xiaohua Huang , Thomas B Ryder , Paul Kaplan
发明人： Xiaohua Huang , Thomas B Ryder , Paul Kaplan
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2535/125 , C12Q2565/501 , C12Q2525/161 , C12Q2521/131
摘要： A method for determining the genotype of one or more individuals at a polymorphic locus employs amplification of a region of DNA using primers containing tags and hybridization of the products to one or more probes on a solid support. The genotype or ratio of alleles is identified from the pattern of hybridization. The method can also be used to determine the frequency of different alleles in a population.
摘要翻译： 用于确定多态性位点处的一个或多个个体的基因型的方法使用含有标签的引物和使用产物与固体支持物上的一种或多种探针杂交来扩增DNA区域。 从杂交模式中鉴定等位基因的基因型或比例。 该方法也可用于确定群体中不同等位基因的频率。
-
公开(公告)号：US5712385A
公开(公告)日：1998-01-27
申请号：US479852
申请日：1995-06-07
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.
-
公开(公告)号：US06582938B1
公开(公告)日：2003-06-24
申请号：US09854317
申请日：2001-05-11
申请人： Xing Su , Helin Dong , Thomas B. Ryder
发明人： Xing Su , Helin Dong , Thomas B. Ryder
IPC分类号： C12P1934
CPC分类号： C12Q1/6865
摘要： The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction.
摘要翻译： 本发明涉及核酸，优选mRNA的扩增。 将引物和启动子加入要扩增的靶序列中，然后在体外转录反应中扩增靶。
-
公开(公告)号：US5856088A
公开(公告)日：1999-01-05
申请号：US462646
申请日：1995-06-05
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type from other viruses.
摘要翻译： 扩增寡核苷酸和杂交测定探针，将人类免疫缺陷病毒类型与其他病毒区分开来。
-
公开(公告)号：US06806047B2
公开(公告)日：2004-10-19
申请号：US09776770
申请日：2001-02-06
申请人： Martin J. Goldberg , Govinda Rao S. Yelagalawadi , Eugene Yuji Tanimoto , Huu Minh Tran , Helin Dong , David Lockhart , Thomas B. Ryder , Janet A. Warrington , Jody Beecher
发明人： Martin J. Goldberg , Govinda Rao S. Yelagalawadi , Eugene Yuji Tanimoto , Huu Minh Tran , Helin Dong , David Lockhart , Thomas B. Ryder , Janet A. Warrington , Jody Beecher
IPC分类号： C12Q168
CPC分类号： C12Q1/682 , C12Q1/6804 , Y10S436/807 , C12Q2565/501 , C12Q2563/131 , C12Q2527/125 , C12Q2563/107 , C12Q2563/125 , C12Q2563/143 , C12Q2537/125
摘要： Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.
-
公开(公告)号：US06649749B2
公开(公告)日：2003-11-18
申请号：US09766095
申请日：2001-01-20
IPC分类号： C07H2104
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.
摘要翻译： 扩增寡核苷酸和杂交测定探针，将1型人类免疫缺陷病毒与其他病毒区分开来。
-
9.发明授权Methods of amplifying nucleic acids using promoter-containing primer sequence 失效使用含有启动子的引物序列扩增核酸的方法
公开(公告)号：US5766849A
公开(公告)日：1998-06-16
申请号：US345861
申请日：1994-11-28
申请人： Sherrol H. McDonough , Daniel L. Kacian , Nanibhushan Dattagupta , Diane L. McAllister , Philip W. Hammond , Thomas B. Ryder
发明人： Sherrol H. McDonough , Daniel L. Kacian , Nanibhushan Dattagupta , Diane L. McAllister , Philip W. Hammond , Thomas B. Ryder
CPC分类号： C12Q1/6865 , C07K14/35 , C12N15/1096 , C12Q1/689
摘要： A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
摘要翻译： 提供了一种用于在基本上恒定温度，离子强度和pH条件下自动催化靶核酸序列的多个拷贝的方法，组合物和试剂盒，其中靶序列的多个RNA拷贝自动催化产生另外的拷贝，使用封闭和 未封闭的引物和/或启动子 - 引物以引发DNA和RNA合成，优选地减少非特异性产物形成。 本发明可用于产生核酸靶序列的拷贝，目的包括在临床，环境，法医学和相似样品中定量特异性核酸序列的测定，克隆和产生探针。
-
公开(公告)号：US5612200A
公开(公告)日：1997-03-18
申请号：US218990
申请日：1994-03-28
CPC分类号： C12Q1/6848
摘要： Method for destroying the ability of a nucleic acid to be amplified, comprising the step of contacting the nucleic acid with bleach in an amount and for a time sufficient to inhibit the ability of that nucleic acid to be amplified in an amplification reaction.
摘要翻译： 用于破坏待扩增的核酸的能力的方法，包括使所述核酸与漂白剂以足以抑制所述核酸在扩增反应中扩增的能力的量和时间相接触的步骤。
-
-
-
-
-
-
-
-
-
搜索结果
16 条记录 (耗时 0.018 秒)
