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公开(公告)号：US08828736B2
公开(公告)日：2014-09-09
申请号：US13173180
申请日：2011-06-30
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0645 , B01L2300/0864 , C12M35/02 , Y10T436/2575
摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.
摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。
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2.发明授权Methods and devices for immobilization of single particles in a virtual channel in a hydrodynamic trap 有权用于在流体动力学陷阱中将虚拟通道中的单个颗粒固定的方法和装置公开(公告)号：US08815177B2
公开(公告)日：2014-08-26
申请号：US12812974
申请日：2009-01-21
CPC分类号： G01N33/5005 , B01F3/0807 , B01F5/0471 , B01F13/0059 , B01L3/502707 , B01L3/502753 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0688 , B81B2201/058 , B81C1/00047 , B81C1/00087 , B81C1/00119 , B81C1/00404 , B81C2201/0133 , B81C2201/0142 , Y10T137/9464 , Y10T428/24479
摘要： Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.
摘要翻译： 本文公开了固定颗粒的方法，其包括将含有颗粒的样品流体的流动聚焦到虚拟通道中，该虚拟通道朝向微流体通道中的未占据的流体动力阱捕获，使得颗粒流入流体动力阱并固定在其中。 还公开了微流体装置，其包括具有至少一个流体动力捕集器，至少一个聚焦流体入口的至少一个微通道，所述聚焦流体入口在流体动力捕集器的上游，使得其中引入的聚焦流体导致样品的虚拟通道 存在时优先流向流体动力阱的流体。
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公开(公告)号：US08585916B2
公开(公告)日：2013-11-19
申请号：US12812986
申请日：2009-01-21
IPC分类号： B31D3/00
CPC分类号： G01N33/5005 , B01F3/0807 , B01F5/0471 , B01F13/0059 , B01L3/502707 , B01L3/502753 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0688 , B81B2201/058 , B81C1/00047 , B81C1/00087 , B81C1/00119 , B81C1/00404 , B81C2201/0133 , B81C2201/0142 , Y10T137/9464 , Y10T428/24479
摘要： Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.
摘要翻译： 本文公开的方法是在衬底中的两个各向同性湿蚀刻特征之间制造所需高度和/或宽度的微孔的方法，其包括使用蚀刻剂和小于2×一组的掩模距离的单层各向同性湿蚀刻两个特征 蚀刻深度。 本文还公开了使用包括微孔的微孔和微流体装置的方法。
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4.发明授权Particle sorter comprising a fluid displacer in a closed-loop fluid circuit 有权颗粒分选机包括在闭环流体回路中的流体置换器公开(公告)号：US08162149B1
公开(公告)日：2012-04-24
申请号：US12424758
申请日：2009-04-16
IPC分类号： B07C5/38
CPC分类号： B01L3/502761 , B01L3/502784 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2300/088 , B01L2400/0439 , B01L2400/0487 , G01N15/1484 , G01N2015/1006 , G01N2015/149
摘要： Disclosed herein are methods and devices utilizing a fluid displacer in a closed-loop fluid circuit.
摘要翻译： 本文公开了在闭环流体回路中利用流体置换器的方法和装置。
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公开(公告)号：US20120004144A1
公开(公告)日：2012-01-05
申请号：US13173180
申请日：2011-06-30
IPC分类号： C40B60/12
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0645 , B01L2300/0864 , C12M35/02 , Y10T436/2575
摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.
摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。
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公开(公告)号：US20110028351A1
公开(公告)日：2011-02-03
申请号：US12812974
申请日：2009-01-21
IPC分类号： C40B50/18 , G01N33/543
CPC分类号： G01N33/5005 , B01F3/0807 , B01F5/0471 , B01F13/0059 , B01L3/502707 , B01L3/502753 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0688 , B81B2201/058 , B81C1/00047 , B81C1/00087 , B81C1/00119 , B81C1/00404 , B81C2201/0133 , B81C2201/0142 , Y10T137/9464 , Y10T428/24479
摘要： Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.
摘要翻译： 本文公开了固定颗粒的方法，其包括将含有颗粒的样品流体的流动聚焦到虚拟通道中，该虚拟通道朝向微流体通道中的未占据的流体动力阱捕获，使得颗粒流入流体动力阱并固定在其中。 还公开了微流体装置，其包括具有至少一个流体动力捕集器，至少一个聚焦流体入口的至少一个微通道，所述聚焦流体入口在流体动力捕集器的上游，使得其中引入的聚焦流体导致样品的虚拟通道 存在时优先流向流体动力阱的流体。
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公开(公告)号：US20110024368A1
公开(公告)日：2011-02-03
申请号：US12812986
申请日：2009-01-21
CPC分类号： G01N33/5005 , B01F3/0807 , B01F5/0471 , B01F13/0059 , B01L3/502707 , B01L3/502753 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0688 , B81B2201/058 , B81C1/00047 , B81C1/00087 , B81C1/00119 , B81C1/00404 , B81C2201/0133 , B81C2201/0142 , Y10T137/9464 , Y10T428/24479
摘要： Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.
摘要翻译： 本文公开的方法是在衬底中的两个各向同性湿蚀刻特征之间制造所需高度和/或宽度的微孔的方法，其包括使用蚀刻剂和小于2×一组的掩模距离的单层各向同性湿蚀刻两个特征 蚀刻深度。 本文还公开了使用包括微孔的微孔和微流体装置的方法。
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