Product and process for transformation of thraustochytriales microorganisms
    1.
    发明申请
    Product and process for transformation of thraustochytriales microorganisms 有权
    破囊壶菌微生物转化的产物和方法

    公开(公告)号:US20050112719A1

    公开(公告)日:2005-05-26

    申请号:US11021712

    申请日:2004-12-22

    摘要: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

    摘要翻译: 公开了乙酰乳酸合酶,乙酰乳酸合成酶调节区,α-微管蛋白启动子,破囊壶菌聚酮合成酶(PKS)系统的启动子和来自破囊壶菌菌体的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体,以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸,以及用于在裂囊酵素微生物中的基因的缺失,突变或失活。