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    Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation
    2.
    发明授权
    Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation 失效
    标记的肽，蛋白质和抗体以及用于其制备的方法和中间体

    公开(公告)号：US07662644B2

    公开(公告)日：2010-02-16

    申请号：US11673909

    申请日：2007-02-12

    申请人： Klaus M. Hahn ,  Alexei Toutchkine ,  Rajeev Muthyala ,  Vadim Kraynov ,  Steven J. Bark ,  Dennis R. Burton ,  Chester Chamberlain

    发明人： Klaus M. Hahn ,  Alexei Toutchkine ,  Rajeev Muthyala ,  Vadim Kraynov ,  Steven J. Bark ,  Dennis R. Burton ,  Chester Chamberlain

    IPC分类号： G01N33/533 ,  G01N33/532 ,  G01N33/53 ,  C07K1/10 ,  C07D277/62

    CPC分类号： C07K1/13 ,  C07K1/1077

    摘要： The invention provides peptide synthons having protected functional groups for attachment of desired moieties (e.g. functional molecules or probes). Also provided are peptide conjugates prepared from such synthons, and synthon and conjugate preparation methods including procedures for identifying optimum probe attachment sites. Biosensors are provided having functional molecules that can locate and bind to specific biomolecules within living cells. Biosensors can detect chemical and physiological changes in those biomolecules as living cells are moving, metabolizing and reacting to its environment. Methods are included for detecting GTP activation of a Rho GTPase protein using polypeptide biosensors. When the biosensor binds GTP-activated Rho GTPase protein, an environmentally sensitive dye emits a signal of a different lifetime, intensity or wavelength than when not bound. New fluorophores whose fluorescence responds to environmental changes are also provided that have improved detection and attachment properties, and that can be used in living cells, or in vitro.

    摘要翻译： 本发明提供具有用于连接所需部分（例如功能分子或探针）的保护的官能团的肽合成子。 还提供了由这种合成子制备的肽缀合物，以及合成子和缀合物制备方法，包括鉴定最佳探针附着位点的程序。 生物传感器具有能够定位并结合活细胞内的特定生物分子的功能分子。 生物传感器可以检测生物分子中的化学和生理变化，因为活细胞正在移动，代谢并对其环境作出反应。 包括使用多肽生物传感器检测Rho GTPase蛋白的GTP活化的方法。 当生物传感器结合GTP激活的Rho GTPase蛋白质时，环境敏感的染料会发出与未结合不同的寿命，强度或波长的信号。 还提供了荧光对环境变化的响应的新荧光团，其具有改进的检测和附着性质，并且可用于活细胞或体外。