公开(公告)号：US20050033019A1

公开(公告)日：2005-02-10

申请号：US10495491

申请日：2002-11-21

申请人： Francois Mallet ,  Valerie Cheynet ,  Guy Oriol ,  Laure Allard ,  Armelle Novelli-Rousseau

发明人： Francois Mallet ,  Valerie Cheynet ,  Guy Oriol ,  Laure Allard ,  Armelle Novelli-Rousseau

IPC分类号： C12N15/09 ,  C07K14/16 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N11/06 ,  C12N15/48 ,  C12P21/02 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/705

CPC分类号： C07K14/005 ,  C12N11/06 ,  C12N2740/16222

摘要： The invention concerns a nucleotide sequence coding for a modified protein of interest, said protein of interest having, after purification and immobilization, at least the same biological activity as the native protein of interest and being directly usable, said sequence comprising at least a gene coding for said protein of interest, a nucleotide fragment, called polyK, coding for a succession of at least six lysine residues, and a nucleotide fragment, called polyH, coding for a succession of at least six histidine residues; a vector comprising such a sequence; and a method for obtaining a purifiable and immobilized modified protein of interest.

摘要翻译： 本发明涉及编码目的修饰的蛋白质的核苷酸序列，所述目的蛋白质在纯化和固定后至少具有与目的天然蛋白质相同的生物学活性且可直接使用，所述序列至少包含编码 对于所述感兴趣的蛋白质，编码一系列至少六个赖氨酸残基的称为polyK的核苷酸片段和称为polyH的核苷酸片段，其编码连续至少六个组氨酸残基; 包含这样的序列的载体; 以及获得可纯化和固定的目标改性蛋白质的方法。

公开(公告)号：US20050069933A1

公开(公告)日：2005-03-31

申请号：US10940964

申请日：2004-09-15

申请人： Valerie Cheynet-Sauvion ,  Nadege Arnaud-Barbe ,  Guy Oriol ,  William McAllister ,  Bernard Mandrand ,  Francois Mallet

发明人： Valerie Cheynet-Sauvion ,  Nadege Arnaud-Barbe ,  Guy Oriol ,  William McAllister ,  Bernard Mandrand ,  Francois Mallet

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22 ,  C12N15/74 ,  C12Q1/70

CPC分类号： C12Q1/6865 ,  C12N9/127 ,  C12Q2521/119

摘要： RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.

摘要翻译： 可以使用核苷酸试剂作为启动子来转录RNA。 通过已知DNA依赖性的RNA聚合酶，例如噬菌体T7的RNA聚合酶，或者通过新的突变的RNA聚合酶，使试剂可以使RNA不经序列指定而没有蛋白质辅因子转录， 当基质是RNA时，与基质是DNA相比，合成具有更高收率的多核苷酸基质的转录产物的能力。 可以通过对野生型RNA聚合酶的编码基因进行突变，然后通过选择具有该能力的突变的RNA聚合酶来获得这种类型的RNA聚合酶。 本发明可以应用于RNA的检测，合成或定量。

公开(公告)号：US20090148454A1

公开(公告)日：2009-06-11

申请号：US12087893

申请日：2007-02-09

申请人： Francois Mallet ,  Guy Oriol ,  Valerie Cheynet

发明人： Francois Mallet ,  Guy Oriol ,  Valerie Cheynet

IPC分类号： A61K39/395 ,  C07K14/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/08 ,  A61K31/7088 ,  A61K38/16 ,  C12Q1/70 ,  C07K16/18 ,  C12P21/00

CPC分类号： C07K14/005 ,  A61K38/162 ,  A61K39/12 ,  A61K39/21 ,  C07K16/1036 ,  C07K2317/34 ,  C12N7/00 ,  C12N2740/10022 ,  C12N2740/10034 ,  C12N2740/10063 ,  G01N2333/15

摘要： The invention relates to a peptide domain required for interaction between the envelope of a virus pertaining to the HERV-W interference group and a hASCT receptor, comprising an N end point and a C end point. Said peptide domain is defined, at the N end point thereof, by a pattern formed by the amino acids L (Z)-proline-cysteine-X-cysteine in which Z is any amino acid, is a whole number between 2 and 30, and X is any amino acid, and at the C end point thereof, by a pattern formed by the amino acids serine-aspartic acid-Xa-Xb-Xc-Xd-Xe-aspartic acid-Xf-Xg-(Z) in which Xa, Xb, Xc, Xd, Xe, Xf, Xg are any amino acids, Z is any amino acid, B is a whole number between 15 and 25, preferably 20. The peptide domain comprises, between the N end point and the C end point, at least one pattern selected from the following patterns: a pattern formed by the amino acids cysteine-X2-X3-X4-X5-X6-cysteine in which X2, X3, X4, X5, X6 are any amino acids, and a pattern formed by the amino acids cysteine-X7-X8-X9-X10-X11-X12-X13-X14-X15-cysteine-trytophane in which X7, X8, X9, X10, X11, X12, X13, X14, X15 are any amino acids.

摘要翻译： 本发明涉及涉及HERV-W干扰组的病毒包膜与hASCT受体之间的相互作用所需的肽结构域，其包含N个终点和C个终点。 所述肽结构域在N末端被由氨基酸L（Z） - 脯氨酸 - 半胱氨酸-X-半胱氨酸形成的图案定义，其中Z是任何氨基酸，其整数为2〜30， X为任何氨基酸，C端为氨基酸丝氨酸 - 天冬氨酸-Xa-Xb-Xc-Xd-Xe-天冬氨酸-Xf-Xg-（Z）形成的图案，其中 Xa，Xb，Xc，Xd，Xe，Xf，Xg是任何氨基酸，Z是任何氨基酸，B是15和25之间的整数，优选为20.肽结构域包括N端和C 从以下图案中选择的至少一种图案：由X2，X3，X4，X5，X6是任何氨基酸的氨基酸半胱氨酸-X 2 -X 3 -X 4 -X 5 -X 6半胱氨酸形成的图案，以及 其中X7，X8，X9，X10，X11，X12，X13，X14，X15的氨基酸半胱氨酸-X7-X8-X9-X10-X11-X12-X13-X14-X15-半胱氨酸 - 替硝唑形成的图案是 任何氨基酸。