利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

7 条记录 (耗时 0.012 秒)

    Process for preparing lincomycin
    3.
    发明授权
    Process for preparing lincomycin 失效
    林可霉素的制备方法

    公开(公告)号：US4271266A

    公开(公告)日：1981-06-02

    申请号：US477766

    申请日：1974-06-10

    申请人： Malcolm E. Bergy ,  John H. Coats ,  Vedpal S. Malik

    发明人： Malcolm E. Bergy ,  John H. Coats ,  Vedpal S. Malik

    IPC分类号： C12P19/64 ,  C07H15/16 ,  C12N1/20 ,  C12P1/06 ,  C12R1/465

    CPC分类号： C07H15/16 ,  Y10S435/886

    摘要： Microbiological process for preparing the antibiotic lincomycin at temperatures ranging from 18.degree. C. to 45.degree. C. using the newly discovered microorganism Streptomyces vellosus. The subject process advantageously results in the preparation of lincomycin without the concomitant production of lincomycin B (4'-depropyl-4'-ethyllincomycin). The absence of lincomycin B production results in increased lincomycin recovery efficiency.

    摘要翻译： 使用新发现的微生物链霉菌（Streptomyces vellosus）在18℃至45℃的温度范围内制备抗生素林可霉素的微生物过程。 本发明方法有利地导致林可霉素的制备，而不伴随产生林可霉素B（4'-去丙基-4'-乙基林霉素）。 林可霉素B生产的缺乏导致林可霉素回收效率提高。

    Thermophilic denitrification of tobacco
    5.
    发明授权
    Thermophilic denitrification of tobacco 失效
    烟草的嗜热反硝化

    公开(公告)号：US4685478A

    公开(公告)日：1987-08-11

    申请号：US307602

    申请日：1981-10-01

    申请人： Vedpal S. Malik ,  Bernard A. Semp ,  Hernan G. Bravo ,  Daniel M. Teng

    发明人： Vedpal S. Malik ,  Bernard A. Semp ,  Hernan G. Bravo ,  Daniel M. Teng

    IPC分类号： C12P13/00 ,  A24B15/00 ,  A24B15/20 ,  A24B15/24

    CPC分类号： A24B15/20

    摘要： High temperature processes and thermophilic organisms for use in those processes for reducing the levels of certain nitrogen-containing compounds in tobacco materials. Tobacco materials are contacted with at least one thermophilic organism characterized by an anaerobic, dissimilatory, metabolic pathway for denitrification of tobacco materials under anaerobic and high temperature conditions that promote such metabolism. Tobacco materials treated in accordance with these high temperature processes and thermophilic organisms, when incorporated into a smoking product, deliver a significantly reduced amount of oxide of nitrogen in smoke. Moreover, such tobacco materials also afford the product of other tobacco products having lower amounts of nitrates and other nitrogen-containing compounds.

    摘要翻译： 用于降低烟草材料中某些含氮化合物含量的那些方法中的高温过程和嗜热生物。 烟草材料与至少一种嗜热生物体接触，其特征在于在厌氧和高温条件下促进这种代谢的厌氧，异化，代谢途径用于烟草材料的脱氮。 根据这些高温过程和嗜热生物体处理的烟草材料当被掺入吸烟产品中时，可以显着减少烟气中氮的氧化物量。 此外，这种烟草材料还提供具有较低量硝酸盐和其它含氮化合物的其它烟草制品的产品。

    Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same
    7.
    发明授权
    Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same 失效
    pBR322和Streptomyces质粒的杂交质粒和含有其的大肠杆菌

    公开(公告)号：US4343906A

    公开(公告)日：1982-08-10

    申请号：US068418

    申请日：1979-08-21

    申请人： Fritz Reusser ,  Vedpal S. Malik

    发明人： Fritz Reusser ,  Vedpal S. Malik

    IPC分类号： C12N15/76 ,  C12N1/20

    CPC分类号： C12N15/76

    摘要： A process for cloning DNA into a suitable host, which comprises fragmenting said DNA to obtain fragmented DNA, ligating said fragmented DNA into a suitable vector to obtain chimeric (hybrid) DNA, and transforming said chimeric DNA into said ultimate host. By this process, the useful chemical plasmid pUC3, which is obtainable from a biologically pure culture of the microorganism Streptomyces sp. 3022a, NRRL 11441, is cloned into the well-known bacterium E. coli HB101. This cloning of pUC3 into E. coli HB101 enables the production of large amounts of plasmid pUC3 DNA. pUC3 is useful as a cloning vehicle in recombinant DNA work. For example, using recombinant DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC3 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

    摘要翻译： 将DNA克隆到合适的宿主中的方法，其包括将所述DNA片段化以获得片段化的DNA，将所述片段化DNA连接到合适的载体中以获得嵌合（杂交）DNA，并将所述嵌合DNA转化到所述最终宿主中。 通过这个过程，可以从微生物链霉菌Streptomyces sp。的生物纯培养物获得的有用的化学质粒pUC3。 3022a，NRRL 11441克隆到公知的大肠杆菌HB101中。 将pUC3克隆到大肠杆菌HB101中能够产生大量的质粒pUC3DNA。 pUC3可用作重组DNA工作中的克隆载体。 例如，使用重组DNA方法，将期望的基因，例如胰岛素基因插入到pUC3中，然后将得到的质粒转化成合适的宿主微生物，培养后产生所需的胰岛素。