公开(公告)号：US10655120B2

公开(公告)日：2020-05-19

申请号：US14006369

申请日：2012-03-21

申请人： Che John Connon ,  Richard Anthony Cave ,  Vitaliy Khutoryanskiy ,  Bernice Wright

发明人： Che John Connon ,  Richard Anthony Cave ,  Vitaliy Khutoryanskiy ,  Bernice Wright

IPC分类号： C12N11/10 ,  A61K35/30 ,  A61K9/06 ,  A61K9/00 ,  A01N1/02 ,  A61K47/36 ,  A61L27/52 ,  A61L27/38 ,  A61K38/18

摘要： The present invention relates to hydrogels which may be used to encapsulate or entrap live cells. The invention further relates to methods for transporting live cells which are encapsulated or entrapped within hydrogels from a first location to a second location. The invention further relates to method of treating a wound, disease or tissue injury, e.g. an ocular injury or a damaged ocular surface in a subject using a hydrogel comprising corneal stem cells. The hydrogels used in such methods may be ones which have been transported from a first location to a second location.

公开(公告)号：US20140072601A1

公开(公告)日：2014-03-13

申请号：US14006369

申请日：2012-03-21

申请人： Che John Connon ,  Richard Anthony Cave ,  Vitaliy Khutoryanskiy ,  Bernice Wright

发明人： Che John Connon ,  Richard Anthony Cave ,  Vitaliy Khutoryanskiy ,  Bernice Wright

IPC分类号： C12N11/10 ,  A61K38/18 ,  A61K35/30

CPC分类号： C12N11/10 ,  A01N1/0231 ,  A61K9/0051 ,  A61K9/06 ,  A61K35/30 ,  A61K38/18 ,  A61K47/36 ,  A61L27/3834 ,  A61L27/52 ,  A61L2400/04 ,  A61L2430/16

摘要： The present invention relates to hydrogels which may be used to encapsulate or entrap live cells. The invention further relates to methods for transporting live cells which are encapsulated or entrapped within hydrogels from a first location to a second location. The invention further relates to method of treating a wound, disease or tissue injury, e.g. an ocular injury or a damaged ocular surface in a subject using a hydrogel comprising corneal stem cells. The hydrogels used in such methods may be ones which have been transported from a first location to a second location.

摘要翻译： 本发明涉及可用于包封或捕获活细胞的水凝胶。 本发明还涉及用于输送活细胞的方法，所述活细胞被包封或捕获在水凝胶中从第一位置到第二位置。 本发明还涉及治疗伤口，疾病或组织损伤的方法，例如， 使用包含角膜干细胞的水凝胶在受试者中的眼部损伤或损伤的眼表面。 在这种方法中使用的水凝胶可以是已经从第一位置运输到第二位置的水凝胶。

公开(公告)号：US20130018110A1

公开(公告)日：2013-01-17

申请号：US13574053

申请日：2011-01-20

申请人： Vitaliy Khutoryanskiy ,  Olga Khutoryanskaya ,  Joseph Peter Cook ,  Glenn William Goodall

发明人： Vitaliy Khutoryanskiy ,  Olga Khutoryanskaya ,  Joseph Peter Cook ,  Glenn William Goodall

IPC分类号： C08L29/04 ,  C08K3/20 ,  C08L33/02 ,  C08L35/08 ,  C08L33/26 ,  C08L33/24 ,  A61K47/32 ,  C08J3/24

CPC分类号： C08J3/075 ,  C08J2329/00 ,  C08J2329/04 ,  C08J2333/00 ,  C08J2339/00

摘要： The invention provides a method for preparing a hydrogel from a hydrophilic polymer having one or more functional groups which are capable of co-reacting in a condensation reaction which method comprises the steps of: (i) preparing a solution of the polymer; (ii) heating the solution to a temperature sufficient for the condensation reaction to take place for a period of time sufficient for the hydrogel to cross-link wherein where the hydrophilic polymer comprises a first and a second hydrophilic polymer, step (i) comprises a step of mixing the hydrophilic polymers to prepare a homogeneous intimate mixture of the polymers; and wherein the heating step (ii) is carried out at a pressure greater than atmospheric pressure. The method of the invention is advantageous because it is relatively low cost, it is a safer procedure with less health & safety concerns, it is carried out in a liquid state and is not limited by film thickness.

摘要翻译： 本发明提供了一种从具有一个或多个能够在缩合反应中共反应的官能团的亲水聚合物制备水凝胶的方法，该方法包括以下步骤：（i）制备聚合物溶液; （ii）将溶液加热到足以使缩合反应发生足以使水凝胶交联的时间，其中亲水性聚合物包含第一和第二亲水性聚合物，其中步骤（i）包括 混合亲水性聚合物以制备聚合物均匀的紧密混合物的步骤; 并且其中所述加热步骤（ii）在大于大气压的压力下进行。 本发明的方法是有利的，因为它的成本相对较低，它是一种更安全的方法，具有较少的健康和安全性，它以液体状态进行并且不受膜厚度的限制。