公开(公告)号：US20110217773A1

公开(公告)日：2011-09-08

申请号：US12716141

申请日：2010-03-02

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： C12N5/10 ,  C12N5/07 ,  C12N5/071 ,  C12N5/02

CPC分类号： C12N5/10

摘要： The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.

摘要翻译： 本发明提供了一种在体外传代培养和扩增的去分化软骨细胞中恢复天然软骨细胞表型和功能和/或增加II型胶原以及聚集蛋白聚糖mRNA表达水平和GAG积聚水平的方法，其包括培养所述 用包含II型胶原的培养基或其生物活性肽片段或具有或不具有生长因子的类似物在体外进行去分化软骨细胞，其中II型胶原或其生物活性肽片段或类似物是有效的 和/或增加所述去分化软骨细胞中II型胶原和聚集蛋白聚糖表达水平和GAG积累水平的软骨细胞表型和功能。

公开(公告)号：US20060190091A1

公开(公告)日：2006-08-24

申请号：US11063074

申请日：2005-02-22

申请人： Wen-Fu Lai ,  Li-Chern Pan ,  Pau-Yee Lim ,  Jung-Chou Oung ,  Chun-Wei Chen

发明人： Wen-Fu Lai ,  Li-Chern Pan ,  Pau-Yee Lim ,  Jung-Chou Oung ,  Chun-Wei Chen

IPC分类号： A61F2/28

CPC分类号： A61L27/50 ,  A61L27/06 ,  A61L27/24 ,  A61L27/34 ,  A61L2430/02 ,  C08L89/06

摘要： This invention relates to a bone implant that includes a bioinert substrate covered with a ceramic layer containing a plurality of indentations. The total surface area of the indentations is 30-70% of the total surface area of the ceramic layer. This invention also relates to a method of preparing such a bone implant. The method includes: (1) affixing a ceramic layer on the surface of a bioinert substrate; (2) forming a plurality of indentations in the ceramic layer, wherein the total surface area of the indentations is 30-70% of the total surface area of the ceramic layer; and (3) immobilizing a biopolymer onto the ceramic layer via covalent bonding.

摘要翻译： 本发明涉及一种骨植入物，其包括用包含多个凹陷的陶瓷层覆盖的生物惰性基底。 压痕的总表面积为陶瓷层总表面积的30-70％。 本发明还涉及一种制备这种骨植入物的方法。 该方法包括：（1）在生物惰性基材的表面上固定陶瓷层; （2）在陶瓷层中形成多个凹陷，其中凹陷的总表面积为陶瓷层的总表面积的30-70％; 和（3）通过共价键将生物聚合物固定在陶瓷层上。

公开(公告)号：US08431399B2

公开(公告)日：2013-04-30

申请号：US12716141

申请日：2010-03-02

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12N5/10

摘要： The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.

摘要翻译： 本发明提供了一种在体外传代培养和扩增的去分化软骨细胞中恢复天然软骨细胞表型和功能和/或增加II型胶原以及聚集蛋白聚糖mRNA表达水平和GAG积聚水平的方法，其包括培养所述 用包含II型胶原的培养基或其生物活性肽片段或具有或不具有生长因子的类似物在体外进行去分化软骨细胞，其中II型胶原或其生物活性肽片段或类似物是有效的 和/或增加所述去分化软骨细胞中II型胶原和聚集蛋白聚糖表达水平和GAG积累水平的软骨细胞表型和功能。

公开(公告)号：US20110262486A1

公开(公告)日：2011-10-27

申请号：US12799343

申请日：2010-04-22

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： A61K9/00 ,  A61K33/24 ,  A61K35/12 ,  A61K38/39

CPC分类号： A61K33/24 ,  A61L27/34 ,  A61L27/3821 ,  A61L27/44 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/414 ,  A61L2430/02 ,  C08L89/06

摘要： The invention discloses a bone implant and a manufacturing method thereof. The manufacturing method of the bone implant comprises a step of coating or mixing type II collagen with at least one porous bone material comprising metals, bio-ceramics, natural biopolymers and synthetic polymers. Another manufacturing method of the bone implant comprises the steps of loading type II collagen with or without at least one porous bone material in a container, and lyophilizing the type II collagen to generate a type II collagen sponge construct with or without the porous bone material as the bone material. The manufactured bone implants are effective, with or without loading cells having differentiation tendency towards osteogenesis, to facilitate bone repair upon introduction of the bone implant into various osseous defects.

摘要翻译： 本发明公开了一种骨植入物及其制造方法。 骨植入物的制造方法包括用至少一种包含金属，生物陶瓷，天然生物聚合物和合成聚合物的多孔骨材料涂覆或混合II型胶原的步骤。 骨植入物的另一种制造方法包括以下步骤：将具有或不具有至少一种多孔骨材料的II型胶原蛋白装载在容器中，并将II型胶原冻干以产生具有或不具有多孔骨材料的II型胶原海绵构造物 骨材料。 所制造的骨植入物是有效的，有或没有负载细胞具有分化趋向于成骨的趋势，以便在将骨植入物引入各种骨缺损时促进骨修复。

公开(公告)号：US5876444A

公开(公告)日：1999-03-02

申请号：US626635

申请日：1996-04-01

申请人： Wen-fu Lai

发明人： Wen-fu Lai

IPC分类号： A61F2/00 ,  A61L27/24 ,  A61F2/02

CPC分类号： A61L27/24 ,  A61F2310/00365

摘要： The present invention relates to a process of preparation of a new reconstituted collagen template and further to the reconstituted collagen template being able to cure articular destruction in a mammal.

摘要翻译： 本发明涉及一种制备新的复原胶原模板的方法，并且还涉及能够治愈哺乳动物关节破坏的重建胶原模板。

公开(公告)号：US20130030045A1

公开(公告)日：2013-01-31

申请号：US13567934

申请日：2012-08-06

申请人： Yu-Hui TSAI ,  Yu-Wei Wu ,  Yu-Hsiang Lin ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui TSAI ,  Yu-Wei Wu ,  Yu-Hsiang Lin ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： A61K31/353 ,  A61P19/10

CPC分类号： A61K31/353

摘要： The invention discloses a method of preventing and treating osteoporosis. The method comprises a step of enhancing osteoblast differentiation and inhibiting osteoclast differentiation by administering a pharmaceutically effective amount of a composition comprising 6-hydroxy flavone compound or 7-methoxy flavone compound, or at least one pharmaceutically acceptable salt thereof to a subject.

摘要翻译： 本发明公开了一种预防和治疗骨质疏松症的方法。 该方法包括通过向受试者施用药学有效量的包含6-羟基黄酮化合物或7-甲氧基黄酮化合物或其至少一种药学上可接受的盐的组合物来增强成骨细胞分化和抑制破骨细胞分化的步骤。