公开(公告)号：US08431399B2

公开(公告)日：2013-04-30

申请号：US12716141

申请日：2010-03-02

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12N5/10

摘要： The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.

摘要翻译： 本发明提供了一种在体外传代培养和扩增的去分化软骨细胞中恢复天然软骨细胞表型和功能和/或增加II型胶原以及聚集蛋白聚糖mRNA表达水平和GAG积聚水平的方法，其包括培养所述 用包含II型胶原的培养基或其生物活性肽片段或具有或不具有生长因子的类似物在体外进行去分化软骨细胞，其中II型胶原或其生物活性肽片段或类似物是有效的 和/或增加所述去分化软骨细胞中II型胶原和聚集蛋白聚糖表达水平和GAG积累水平的软骨细胞表型和功能。

公开(公告)号：US20110217773A1

公开(公告)日：2011-09-08

申请号：US12716141

申请日：2010-03-02

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Chin-Chean Wong ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： C12N5/10 ,  C12N5/07 ,  C12N5/071 ,  C12N5/02

CPC分类号： C12N5/10

摘要： The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.

摘要翻译： 本发明提供了一种在体外传代培养和扩增的去分化软骨细胞中恢复天然软骨细胞表型和功能和/或增加II型胶原以及聚集蛋白聚糖mRNA表达水平和GAG积聚水平的方法，其包括培养所述 用包含II型胶原的培养基或其生物活性肽片段或具有或不具有生长因子的类似物在体外进行去分化软骨细胞，其中II型胶原或其生物活性肽片段或类似物是有效的 和/或增加所述去分化软骨细胞中II型胶原和聚集蛋白聚糖表达水平和GAG积累水平的软骨细胞表型和功能。

公开(公告)号：US20110262486A1

公开(公告)日：2011-10-27

申请号：US12799343

申请日：2010-04-22

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Wen-Fu Lai ,  Shih-Ching Chen

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu ,  Wen-Fu Lai ,  Shih-Ching Chen

IPC分类号： A61K9/00 ,  A61K33/24 ,  A61K35/12 ,  A61K38/39

CPC分类号： A61K33/24 ,  A61L27/34 ,  A61L27/3821 ,  A61L27/44 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/414 ,  A61L2430/02 ,  C08L89/06

摘要： The invention discloses a bone implant and a manufacturing method thereof. The manufacturing method of the bone implant comprises a step of coating or mixing type II collagen with at least one porous bone material comprising metals, bio-ceramics, natural biopolymers and synthetic polymers. Another manufacturing method of the bone implant comprises the steps of loading type II collagen with or without at least one porous bone material in a container, and lyophilizing the type II collagen to generate a type II collagen sponge construct with or without the porous bone material as the bone material. The manufactured bone implants are effective, with or without loading cells having differentiation tendency towards osteogenesis, to facilitate bone repair upon introduction of the bone implant into various osseous defects.

摘要翻译： 本发明公开了一种骨植入物及其制造方法。 骨植入物的制造方法包括用至少一种包含金属，生物陶瓷，天然生物聚合物和合成聚合物的多孔骨材料涂覆或混合II型胶原的步骤。 骨植入物的另一种制造方法包括以下步骤：将具有或不具有至少一种多孔骨材料的II型胶原蛋白装载在容器中，并将II型胶原冻干以产生具有或不具有多孔骨材料的II型胶原海绵构造物 骨材料。 所制造的骨植入物是有效的，有或没有负载细胞具有分化趋向于成骨的趋势，以便在将骨植入物引入各种骨缺损时促进骨修复。

公开(公告)号：US08455254B2

公开(公告)日：2013-06-04

申请号：US12799345

申请日：2010-04-22

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu

IPC分类号： C12N5/00

CPC分类号： A61K38/39 ,  C12N5/0654 ,  C12N2533/54 ,  A61K2300/00

摘要： The invention discloses a method of accelerating osteogenic differentiation and a composition thereof. The method comprises a step of adding type II collagen into stem/progenitor cells or osteoblasts to accelerate the osteogenic differentiation of the added cells, and the composition comprises type II collagen, and stem/progenitor cells or osteoblasts. Type II collagen can accelerate osteogenesis of mesenchymal stem cells (MSCs) much faster than does type I collagen. The said composition is effective to facilitate bone repair upon introduction of the composition into various osseous defects.

摘要翻译： 本发明公开了一种加速成骨分化的方法及其组合物。 该方法包括将II型胶原蛋白加入到干细胞/祖细胞或成骨细胞中以加速添加的细胞的成骨分化，并且组合物包含II型胶原和干/祖细胞或成骨细胞。 II型胶原蛋白可以加速间充质干细胞（MSC）的骨形成比I型胶原蛋白快得多。 所述组合物在将组合物引入各种骨缺损中时有助于促进骨修复。

公开(公告)号：US20110262403A1

公开(公告)日：2011-10-27

申请号：US12799345

申请日：2010-04-22

申请人： Yu-Hui Tsai ,  Li-Hsuan Chiu

发明人： Yu-Hui Tsai ,  Li-Hsuan Chiu

IPC分类号： A61K35/12 ,  A61P19/08 ,  A61K38/16

CPC分类号： A61K38/39 ,  C12N5/0654 ,  C12N2533/54 ,  A61K2300/00

摘要： The invention discloses a method of accelerating osteogenic differentiation and a composition thereof. The method comprises a step of adding type II collagen into stem/progenitor cells or osteoblasts to accelerate the osteogenic differentiation of the added cells, and the composition comprises type II collagen, and stem/progenitor cells or osteoblasts. Type II collagen can accelerate osteogenesis of mesenchymal stem cells (MSCs) much faster than does type I collagen. The said composition is effective to facilitate bone repair upon introduction of the composition into various osseous defects.

摘要翻译： 本发明公开了一种加速成骨分化的方法及其组合物。 该方法包括将II型胶原蛋白加入到干细胞/祖细胞或成骨细胞中以加速添加的细胞的成骨分化，并且组合物包含II型胶原和干/祖细胞或成骨细胞。 II型胶原蛋白可以加速间充质干细胞（MSC）的骨形成比I型胶原蛋白快得多。 所述组合物在将组合物引入各种骨缺损中时有助于促进骨修复。

公开(公告)号：US20100022753A1

公开(公告)日：2010-01-28

申请号：US12179584

申请日：2008-07-25

申请人： Yu-Wei Wu ,  Yu-Hui Tsai

发明人： Yu-Wei Wu ,  Yu-Hui Tsai

IPC分类号： C07K1/00 ,  C07H21/00 ,  C07K2/00

CPC分类号： C07H21/00 ,  C07K1/1077 ,  C07K1/13

摘要： A method for modifying a thiol group is provided. The method includes: providing a first material containing at least one sulfhydryl group; providing a second material containing at least one thiourea group; and reacting the first material with the second material in the presence of copper (II) ions to quickly form a disulfide bond between the sulfhydryl group and the thiourea group, wherein the disulfide bond can be easily reduced.

摘要翻译： 提供了修饰硫醇基团的方法。 该方法包括：提供含有至少一个巯基的第一材料; 提供含有至少一个硫脲基团的第二种材料; 并且在铜（II）离子存在下使第一材料与第二材料反应，以在巯基和硫脲基团之间快速形成二硫键，其中二硫键可以容易地还原。

公开(公告)号：US08133683B2

公开(公告)日：2012-03-13

申请号：US12358884

申请日：2009-01-23

申请人： Yu-Wei Wu ,  Yu-Hui Tsai

发明人： Yu-Wei Wu ,  Yu-Hui Tsai

IPC分类号： G01N33/53

CPC分类号： G01N33/54326 ,  G01N33/58 ,  G01N33/6803

摘要： The invention develops a high-throughput screening method based on charcoal-sorbent peptide binding assay (CPBA) which does not need a solid phase and can simultaneously detect plural target biomolecules in a sample. The method of the invention can achieve high-throughput screening of biomolecules (such as antibodies and antigens) with a molecular weight of more than 10 KD.

摘要翻译： 本发明开发了基于不需要固相并可以同时检测样品中多个目标生物分子的木炭 - 吸附肽结合测定（CPBA）的高通量筛选方法。 本发明的方法可以实现分子量大于10KD的生物分子（如抗体和抗原）的高通量筛选。

公开(公告)号：US20100190189A1

公开(公告)日：2010-07-29

申请号：US12358884

申请日：2009-01-23

申请人： Yu-Wei Wu ,  Yu-Hui Tsai

发明人： Yu-Wei Wu ,  Yu-Hui Tsai

IPC分类号： G01N33/53

CPC分类号： G01N33/54326 ,  G01N33/58 ,  G01N33/6803

摘要： The invention develops a high-throughput screening method based on charcoal-sorbent peptide binding assay (CPBA) which does not need a solid phase and can simultaneously detect plural target biomolecules in a sample. The method of the invention can achieve high-throughput screening of biomolecules (such as antibodies and antigens) with a molecular weight of more than 10 KD.

摘要翻译： 本发明开发了基于不需要固相并可以同时检测样品中多个目标生物分子的木炭 - 吸附肽结合测定（CPBA）的高通量筛选方法。 本发明的方法可以实现分子量大于10KD的生物分子（如抗体和抗原）的高通量筛选。

公开(公告)号：US5148704A

公开(公告)日：1992-09-22

申请号：US746358

申请日：1991-08-16

申请人： Yu-Hui Tsai

发明人： Yu-Hui Tsai

IPC分类号： G01L17/00

CPC分类号： G01L17/00

摘要： A tire monitoring and repairing device comprises a body having a front arm and a rear grip arm interconnected at their upper end with a connecting member to substantially form a U-shape. A pressure gauge is mounted in the front arm and a flash light device is mounted in the rear grip arm. The front arm further has a mouth piece projecting forward to form a pressure inlet. A tire repairing tool is detachably screwed to the mouth piece of the front arm. In addition, a tire tread ruler is provided in the rear grip arm.

摘要翻译： 一种轮胎监视和修理装置，包括具有前臂和后把手的主体，所述前臂和后把手在其上端处与连接构件互相连接，以大致形成U形。 压力表安装在前臂中，闪光灯装置安装在后把手臂中。 前臂还具有向前突出的口部件，以形成压力入口。 轮胎修理工具可拆卸地拧到前臂的口部件上。 此外，在后把手臂中设置轮胎胎面花纹尺。