公开(公告)号：US20140162256A1

公开(公告)日：2014-06-12

申请号：US14008344

申请日：2012-03-30

申请人： Yasuko Rikihisa

发明人： Yasuko Rikihisa

IPC分类号： C12Q1/68 ,  G01N33/569

CPC分类号： G01N33/56911 ,  A61K39/395 ,  C07K14/29 ,  C07K16/1246 ,  C12Q1/689 ,  C12Q1/6893 ,  C12Q2600/16 ,  G01N2333/29 ,  G01N2469/20

摘要： Described herein are improved diagnostic tools for veterinary and human use which can be used for serodiagnosing A. platys in mammals, particularly in members of the Canidae family and in humans. The diagnostic tools are a group of outer membrane proteins of A. platys and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of A. platys and variants thereof referred to hereinafter as the “P44 proteins”, and antibodies to the OMP proteins and the P44 proteins.

摘要翻译： 本文描述的是用于兽医和人类使用的改进的诊断工具，其可用于哺乳动物，特别是在犬科和科人的成员中的血清学检测。 诊断工具是A. platys及其变体的一组外膜蛋白，以下称为“OMP蛋白”，一组A. platys的外膜蛋白及其变体，在下文中称为“P44蛋白” ，以及对OMP蛋白和P44蛋白的抗体。

公开(公告)号：US20090075368A1

公开(公告)日：2009-03-19

申请号：US11452602

申请日：2006-06-14

申请人： Yasuko Rikihisa ,  Norio Ohashi

发明人： Yasuko Rikihisa ,  Norio Ohashi

IPC分类号： C12N15/00 ,  C12N15/11

CPC分类号： A61B18/00 ,  A61B18/02 ,  A61B18/1485 ,  A61K39/00 ,  A61N5/0601 ,  A61N5/062 ,  C07K14/29

摘要： Diagnostic tools for serodiagnosing ehrlichiosis in mammals, particularly in members of the Canidae family and in humans are provided. The diagnostic tools are a group of outer membrane proteins of E. chaffeensis and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of E. canis and variants thereof referred to hereinafter as the “P30F proteins”, and antibodies to the ONP proteins and the P30F proteins. The OMP proteins of E. chaffeensis encompass OMP-1, OMP-1A, OMP1-B, OMP-1C, OMP1-D, OMP1-E, OMP1-F, OMP1-H, OMP-1R, OMP-1S, OMP-1T, OMP-1U, OMP-1V, OMP-1W, OMP-1X, OMP-1Y and OMP-1Z. The P30F proteins of E. canis encompass P30, P30a, P30-1, P30-2, P30-3; P30-4, P30-5, P30-6, P30-7, P30-8, P30-9, P30-10, P30-11, and P30-12. Isolated polynucleotides that encode the E. chaffeensis OMP proteins and isolated polynucleotides that encode the E. canis P30F protein are also provided. The present invention also relates to kits containing reagents for diagnosing human ehrlichiosis and canine ehrlichiosis, and to immunogenic compositions containing one or more OMP proteins or P30F proteins.

摘要翻译： 提供用于哺乳动物，特别是在犬科和人类成员中的艾滋病病毒血清学诊断工具。 诊断工具是一种以下文中称为“OMP蛋白”的下列称为“OMP蛋白”的E.Chaffeensis及其变体的外膜蛋白，一组外膜蛋白及其变体，在下文中称为“P30F蛋白” ，以及针对ONP蛋白和P30F蛋白的抗体。 乙酰胆碱酯酶的OMP蛋白包括OMP-1，OMP-1A，OMP1-B，OMP-1C，OMP1-D，OMP1-E，OMP1-F，OMP1-H，OMP-1R，OMP-1S， 1T，OMP-1U，OMP-1V，OMP-1W，OMP-1X，OMP-1Y和OMP-1Z。 大肠杆菌的P30F蛋白包括P30，P30a，P30-1，P30-2，P30-3; P30-4，P30-5，P30-6，P30-7，P30-8，P30-9，P30-10，P30-11和P30-12。 还提供了编码大肠杆菌OMP蛋白的分离的多核苷酸和编码大肠杆菌P30F蛋白的分离的多核苷酸。 本发明还涉及含有用于诊断人类艾氏杆菌病和犬埃里希氏病的试剂的试剂盒，以及含有一种或多种OMP蛋白质或P30F蛋白质的免疫原性组合物。

公开(公告)号：US06893640B2

公开(公告)日：2005-05-17

申请号：US10314639

申请日：2002-12-09

申请人： Yasuko Rikihisa ,  Norio Ohashi

发明人： Yasuko Rikihisa ,  Norio Ohashi

IPC分类号： A61B18/00 ,  A61B18/02 ,  A61B18/14 ,  A61K39/00 ,  A61N5/06 ,  C07K14/29 ,  C12N1/21 ,  A91K39/00 ,  A91K39/02 ,  A91K39/38

CPC分类号： C07K14/29 ,  A61K39/00 ,  A61N5/0601 ,  A61N5/062

摘要： Diagnostic tools for for serodiagnosing ehrlichiosis in mammals, particularly in members of the Canidae family and in humans are provided. The diagnostic tools are a group of outer membrane proteins of E. chaffeensis and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of E. canis and variants thereof referred to hereinafter as the “P30F proteins”, and antibodies to the OMP proteins and the P30F proteins. The OMP proteins of E. chaffeensis encompass OMP-1, OMP-1A, OMP1-B, OMP-1C, OMP1-D, OMP1-E, OMP1-F, OMP1-H, OMP-1R, OMP-1S, OMP-1T, OMP-1U, OMP-1V, OMP-1W, OMP-1X, OMP-1Y and OMP-1Z. The P30F proteins of E. canis encompass P30, P30a, P30-1, P30-2, P30-3, P30-4, P30-5, P30-6, P30-7, P30-8, P30-9, P30-10, P30-11, and P30-12. Isolated polynucleotides that encode the E. chaffeensis OMP proteins and isolated polynucleotides that encode the E. canis P30F protein are also provided. The present invention also relates to kits containing reagents for diagnosing human ehrlichiosis and canine ehrlichiosis, and to immunogenic compositions containing one or more OMP proteins or P30F proteins.

摘要翻译： 提供了用于哺乳动物，特别是犬科和成年人的哺乳动物血清学诊断工具。 诊断工具是一种以下文中称为“OMP蛋白”的下列称为“OMP蛋白”的E.Chaffeensis及其变体的外膜蛋白，一组外膜蛋白及其变体，在下文中称为“P30F蛋白” ，以及对OMP蛋白和P30F蛋白的抗体。 乙酰胆碱酯酶的OMP蛋白包括OMP-1，OMP-1A，OMP1-B，OMP-1C，OMP1-D，OMP1-E，OMP1-F，OMP1-H，OMP-1R，OMP-1S， 1T，OMP-1U，OMP-1V，OMP-1W，OMP-1X，OMP-1Y和OMP-1Z。 大肠杆菌的P30F蛋白包括P30，P30a，P30-1，P30-2，P30-3，P30-4，P30-5，P30-6，P30-7，P30-8，P30-9，P30- 10，P30-11和P30-12。 还提供了编码大肠杆菌OMP蛋白的分离的多核苷酸和编码大肠杆菌P30F蛋白的分离的多核苷酸。 本发明还涉及含有用于诊断人类艾氏杆菌病和犬埃里希氏病的试剂的试剂盒，以及含有一种或多种OMP蛋白质或P30F蛋白质的免疫原性组合物。

公开(公告)号：US06432649B1

公开(公告)日：2002-08-13

申请号：US09648520

申请日：2000-08-25

申请人： Roger William Stich ,  Yasuko Rikihisa

发明人： Roger William Stich ,  Yasuko Rikihisa

IPC分类号： C12Q168

CPC分类号： C12Q1/689

摘要： Tools and methods for detecting the presence of E. canis and E. chaffeensis in a sample obtained from an animal are provided. The methods employ a polymerase chain reaction and primer sets that are based on the p30 gene of E. canis and the p28 gene of E. chaffeensis. The present invention also relates to the p30 and the p28 primer sets. Each p30 primer set comprises a first primer and the second primer, both of which are from 15 to 35 nucleotides in length. The first p30 primer comprises a sequence which is complementary to a consecutive sequence, within the following sequence: CCA AGTGTCTCAC ATTTTGGTAG CTTCTCAGCT AAAGAAGAAA GCAAATCAAC TGTTGGAGTTTTTGGATTAA AACATGATTG GGATGGAAGT CCAATACTTA AGAATAAACA CGCTGACTTTACTGTTCCAA AC. SEQ ID NO.1. The second p30 primer comprises a sequence which is complementary to the inverse complement of a consecutive sequence contained within the following sequence: GTTACT CAATGGGTGG CCCAAGAATA GAATTCGAAA TATCTTATGA AGCATTCGAC GTAAAAAGTC CTAATATCAA TTATCAAAAT GACGCGCACA GGTACTGCGC TCTATCTCAT CACACATCGG CAGCCAT, SEQ ID NO.2. The first p28 comprises a sequence which is complementary to a consecutive sequenc, within the following sequence : A GTTTTCATAA CAAGTGCATT GATATCACTA ATATCTTCTC TACCTGGAGT ATCATTTTCC GACCCAACAG GTAGTGGTAT TAACGG, SEQ ID NO. 3. The second p28 primer comprises a sequence which is complementary to the inverse complement of a consecutive sequence within one of the following two sequences: CAT TTCTAGGTTT TGCAGGAGCT ATTGGCTACT CAATGGATGG TCCAAGAATA GAGCTTGAAG TATCTTATGA, SEQ ID NO. 4, or C AAGGAAAGTT AGGTTTAAGC TACTCTATAA GCCCAGA, SEQ ID NO. 5.

摘要翻译： 提供了用于检测从动物获得的样品中E. canis和E.Chaffeensis的存在的工具和方法。 该方法采用聚合酶链反应和引物组，其基于大肠杆菌的p30基因和E.Chaffeensis的p28基因。 本发明还涉及p30和p28引物组。 每个p30引物组包含长度为15至35个核苷酸的第一引物和第二引物。 第一个p30引物包含与以下序列中的连续序列互补的序列：CCA AGTGTCTCAC ATTTTGGTAG CTTCTCAGCT AAAGAAGAAA GCAAATCAAC TGTTGGAGTTTTTGGATTAA AACATGATTG GGATGGAAGT CCAATACTTA AGAATAAACA CGCTGACTTTACTGTTCCAA AC。 SEQ ID NO.1。 第二个p30引物包含与以下序列中包含的连续序列的反向互补序列互补的序列：GTTACT CAATGGGTGG CCCAAGAATA GAATTCGAAA TATCTTATGA AGCATTCGAC GTAAAAAGTC CTAATATCAA TTATCAAAAT GACGCGCACA GGTACTGCGC TCTATCTCAT CACACATCGG CAGCCAT，SEQ ID NO.2。 第一个p28包含与以下序列中的连续序列互补的序列：GTTTTCATAA CAAGTGCATT GATATCACTA ATATCTTCTC TACCTGGAGT ATCATTTTCC GACCCAACAG GTAGTGGTAT TAACGG，SEQ ID NO： 第二个p28引物包含与以下两个序列之一中的连续序列的反向互补序列互补的序列：CAT TTCTAGGTTT TGCAGGAGCT ATTGGCTACT CAATGGATGG TCCAAGAATA GAGCTTGAAG TATCTTATGA，SEQ ID NO： 4或C AAGGAAAGTT AGGTTTAAGC TACTCTATAA GCCCAGA，SEQ ID NO： 5。

公开(公告)号：US08784828B2

公开(公告)日：2014-07-22

申请号：US12115490

申请日：2008-05-05

申请人： Yasuko Rikihisa

发明人： Yasuko Rikihisa

IPC分类号： A61K39/02 ,  C07K14/195 ,  C07H21/04

CPC分类号： A61K39/0233 ,  A61K38/00 ,  A61K38/04 ,  A61K2039/552 ,  A61K2039/575 ,  A61K2039/6031 ,  C07K7/08 ,  C07K14/29 ,  G01N33/53 ,  G01N33/56911 ,  G01N33/6854 ,  G01N2333/29 ,  G01N2469/20 ,  Y02A50/402

摘要： The novel omp-1 gene cluster encoding twenty one Ehrlichia ewingii (EE) proteins was isolated and sequenced completely. This invention relates to isolated E. ewingii (EE) polypeptides, isolated polynucleotides encoding EE polypeptides, probes, primers, isolated antibodies and methods of their production, immunogenic compositions and vaccines, as well as methods of using the EE polypeptides, antibodies, probes, and primers for the purpose of diagnosis, therapy and production of vaccines against E. ewingii.

摘要翻译： 分离编码二十一种Ehilichia ewingii（EE）蛋白质的新型omp-1基因簇，并完全测序。 本发明涉及分离的E.Ewingii（EE）多肽，编码EE多肽的分离的多核苷酸，探针，引物，分离的抗体及其生产方法，免疫原性组合物和疫苗，以及使用EE多肽，抗体，探针， 和引物，用于诊断，治疗和生产针对大肠埃希氏芽孢杆菌的疫苗。

公开(公告)号：US07709622B2

公开(公告)日：2010-05-04

申请号：US11452602

申请日：2006-06-14

申请人： Yasuko Rikihisa ,  Norio Ohashi

发明人： Yasuko Rikihisa ,  Norio Ohashi

IPC分类号： C07H21/04 ,  A61K39/00 ,  A61K39/02

CPC分类号： A61B18/00 ,  A61B18/02 ,  A61B18/1485 ,  A61K39/00 ,  A61N5/0601 ,  A61N5/062 ,  C07K14/29

摘要： Diagnostic tools for serodiagnosing ehrlichiosis in mammals, particularly in members of the Canidae family and in humans are provided. The diagnostic tools are a group of outer membrane proteins of E. chaffeensis and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of E. canis and variants thereof referred to hereinafter as the “P30F proteins”, and antibodies to the ONP proteins and the P30F proteins. The OMP proteins of E. chaffeensis encompass OMP-1, OMP-1A, OMP1-B, OMP-1C, OMP1-D, OMP1-E, OMP1-F, OMP1-H, OMP-1R, OMP-1S, OMP-1T, OMP-1U, OMP-1V, OMP-1W, OMP-1X, OMP-1Y and OMP-1Z. The P30F proteins of E. canis encompass P30, P30a, P30-1, P30-2, P30-3; P30-4, P30-5, P30-6, P30-7, P30-8, P30-9, P30-10, P30-11, and P30-12. Isolated polynucleotides that encode the E. chaffeensis OMP proteins and isolated polynucleotides that encode the E. canis P30F protein are also provided. The present invention also relates to kits containing reagents for diagnosing human ehrlichiosis and canine ehrlichiosis, and to immunogenic compositions containing one or more OMP proteins or P30F proteins.

摘要翻译： 提供用于哺乳动物，特别是在犬科和人类成员中的艾滋病病毒血清学诊断工具。 诊断工具是一种以下文中称为“OMP蛋白”的下列称为“OMP蛋白”的E.Chaffeensis及其变体的外膜蛋白，一组外膜蛋白及其变体，在下文中称为“P30F蛋白” ，以及针对ONP蛋白和P30F蛋白的抗体。 乙酰胆碱酯酶的OMP蛋白包括OMP-1，OMP-1A，OMP1-B，OMP-1C，OMP1-D，OMP1-E，OMP1-F，OMP1-H，OMP-1R，OMP-1S， 1T，OMP-1U，OMP-1V，OMP-1W，OMP-1X，OMP-1Y和OMP-1Z。 大肠杆菌的P30F蛋白包括P30，P30a，P30-1，P30-2，P30-3; P30-4，P30-5，P30-6，P30-7，P30-8，P30-9，P30-10，P30-11和P30-12。 还提供了编码大肠杆菌OMP蛋白的分离的多核苷酸和编码大肠杆菌P30F蛋白的分离的多核苷酸。 本发明还涉及含有用于诊断人类艾氏杆菌病和犬埃里希氏病的试剂的试剂盒，以及含有一种或多种OMP蛋白质或P30F蛋白质的免疫原性组合物。

公开(公告)号：US06544517B1

公开(公告)日：2003-04-08

申请号：US09314701

申请日：1999-05-19

申请人： Yasuko Rikihisa ,  Norio Ohashi

发明人： Yasuko Rikihisa ,  Norio Ohashi

IPC分类号： A61K3900

CPC分类号： A61B18/00 ,  A61B18/02 ,  A61B18/1485 ,  A61K39/00 ,  A61N5/0601 ,  A61N5/062 ,  C07K14/29

摘要： Diagnostic tools for for serodiagnosing ehrlichiosis in mammals, particularly in members of the Canidae family and in humans are provided. The diagnostic tools are a group of outer membrane proteins of E. chaffeensis and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of E. canis and variants thereof referred to hereinafter as the “P30F, proteins”, and antibodies to the OMP proteins and the P30F proteins. The OMP proteins of E. chaffeensis encompass OMP-1, OMP-1A, OMP1-B, OMP-1C, OMP1-D, OMP1-E, OMP1-F, OMP1-H, OMP-1R, OMP-1S, OMP-1T, OMP-1U, OMP-1V, OMP-1W, OMP-1X, OMP-1Y and OMP-1Z. The P3OF proteins of E. canis encompass P30, P30a, P30-1, P30-2, P30-3, P30-4, P30-5, P30-6, P30-7, P30-8, P30-9, P30-10, P30-11, and P30-12. Isolated polynucleotides that encode the E. chaffeensis OMP proteins and isolated polynucleotides that encode the E. canis P30F protein are also provided. The present invention also relates to kits containing reagents for diagnosing human ehrlichiosis and canine ehrlichiosis, and to immunogenic compositions containing one or more OMP proteins or P30F proteins.

摘要翻译： 提供了用于哺乳动物，特别是犬科和成年人的哺乳动物血清学诊断工具。 诊断工具是一种以下的称为“OMP蛋白”的下列称为“OMP蛋白”的E.Chaffeensis及其变体的外膜蛋白，一组外膜蛋白及其变体，在下文称为“P30F，蛋白 “以及对OMP蛋白和P30F蛋白的抗体。 乙酰胆碱酯酶的OMP蛋白包括OMP-1，OMP-1A，OMP1-B，OMP-1C，OMP1-D，OMP1-E，OMP1-F，OMP1-H，OMP-1R，OMP-1S， 1T，OMP-1U，OMP-1V，OMP-1W，OMP-1X，OMP-1Y和OMP-1Z。 大肠杆菌的P3OF蛋白包括P30，P30a，P30-1，P30-2，P30-3，P30-4，P30-5，P30-6，P30-7，P30-8，P30-9，P30- 10，P30-11和P30-12。 还提供了编码大肠杆菌OMP蛋白的分离的多核苷酸和编码大肠杆菌P30F蛋白的分离的多核苷酸。 本发明还涉及含有用于诊断人类艾氏杆菌病和犬埃里希氏病的试剂的试剂盒，以及含有一种或多种OMP蛋白质或P30F蛋白质的免疫原性组合物。

公开(公告)号：US07888491B2

公开(公告)日：2011-02-15

申请号：US10901714

申请日：2004-07-29

申请人： Yasuko Rikihisa ,  Norio Ohashi

发明人： Yasuko Rikihisa ,  Norio Ohashi

IPC分类号： C07H21/04 ,  A61K39/12 ,  A61K39/02

CPC分类号： C07K14/29 ,  A61K39/00 ,  A61N5/0601 ,  A61N5/062

摘要： Diagnostic tools for for serodiagnosing ehrlichiosis in mammals, particularly in members of the Canidae family and in humans are provided. The diagnostic tools are a group of outer membrane proteins of E. chaffeensis and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of E. canis and variants thereof referred to hereinafter as the “P30F proteins”, and antibodies to the OMP proteins and the P30F proteins. The OMP proteins of E. chaffeensis encompass OMP-1, OMP-1A, OMP1-B, OMP-1C, OMP1-D, OMP1-E, OMP1-F, OMP1-H, OMP-1R, OMP-1S, OMP-1T, OMP-1U, OMP-1V, OMP-1W, OMP-1X, OMP-1Y and OMP-1Z. The P30F proteins of E. canis encompass P30, P30a, P30-1, P30-2, P30-3, P304, P30-5, P30-6, P30-7, P30-8, P30-9, P30-10, P30-11, and P30-12. Isolated polynucleotides that encode the E. chaffeensis OMP proteins and isolated polynucleotides that encode the E. canis P30F protein are also provided. The present invention also relates to kits containing reagents for diagnosing human ehrlichiosis and canine ehrlichiosis, and to immunogenic compositions containing one or more OMP proteins or P30F proteins.

摘要翻译： 提供了用于哺乳动物，特别是犬科和成年人的哺乳动物血清学诊断工具。 诊断工具是一种以下文中称为“OMP蛋白”的下列称为“OMP蛋白”的E.Chaffeensis及其变体的外膜蛋白，一组外膜蛋白及其变体，在下文中称为“P30F蛋白” ，以及对OMP蛋白和P30F蛋白的抗体。 乙酰胆碱酯酶的OMP蛋白包括OMP-1，OMP-1A，OMP1-B，OMP-1C，OMP1-D，OMP1-E，OMP1-F，OMP1-H，OMP-1R，OMP-1S， 1T，OMP-1U，OMP-1V，OMP-1W，OMP-1X，OMP-1Y和OMP-1Z。 大肠杆菌的P30F蛋白包括P30，P30a，P30-1，P30-2，P30-3，P304，P30-5，P30-6，P30-7，P30-8，P30-9，P30-10， P30-11和P30-12。 还提供了编码大肠杆菌OMP蛋白的分离的多核苷酸和编码大肠杆菌P30F蛋白的分离的多核苷酸。 本发明还涉及含有用于诊断人类艾氏杆菌病和犬埃里希氏病的试剂的试剂盒，以及含有一种或多种OMP蛋白质或P30F蛋白质的免疫原性组合物。

公开(公告)号：US07063846B2

公开(公告)日：2006-06-20

申请号：US10901774

申请日：2004-07-29

申请人： Yasuko Rikihisa ,  Norio Ohashi

发明人： Yasuko Rikihisa ,  Norio Ohashi

IPC分类号： A61K39/40 ,  A61K39/395 ,  A61K39/42

CPC分类号： A61B18/00 ,  A61B18/02 ,  A61B18/1485 ,  A61K39/00 ,  A61N5/0601 ,  A61N5/062 ,  C07K14/29

摘要： Diagnostic tools for for serodiagnosing ehrlichiosis in mammals, particularly in members of the Canidae family and in humans are provided. The diagnostic tools are a group of outer membrane proteins of E. chaffeensis and variants thereof, referred to hereinafter as the “OMP proteins”, a group of outer membrane proteins of E. canis and variants thereof referred to hereinafter as the “P30F proteins”, and antibodies to the OMP proteins and the P30F proteins. The OMP proteins of E. chaffeensis encompass OMP-1, OMP-1A, OMP1-B, OMP-1C, OMP1-D, OMP1-E, OMP1-F, OMP1-H, OMP-1R, OMP-1S, OMP-1T, OMP-1U, OMP-1V, OMP-1W, OMP-1X, OMP-1Y and OMP-1Z. The P30F proteins of E. canis encompass P30, P30a, P30-1, P30-2, P30-3, P30-4, P30-5, P30-6, P30-7, P30-8, P30-9, P30-10, P30-11, and P30-12. Isolated polynucleotides that encode the E. chaffeensis OMP proteins and isolated polynucleotides that encode the E. canis P30F protein are also provided. The present invention also relates to kits containing reagents for diagnosing human ehrlichiosis and canine ehrlichiosis, and to immunogenic compositions containing one or more OMP proteins or P30F proteins.

公开(公告)号：US20050170341A1

公开(公告)日：2005-08-04

申请号：US10138162

申请日：2002-05-02

申请人： Roger Stich ,  Yasuko Rikihisa

发明人： Roger Stich ,  Yasuko Rikihisa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/689

摘要： Tools and methods for detecting the presence of E. canis and E. chaffeensis in a sample obtained from an animal are provided. The methods employ a polymerase chain reaction and primer sets that are based on the p30 gene of E. canis and the p28 gene of E. chaffeensis. The present invention also relates to the p30 and the p28 primer sets. Each p30 primer set comprises a first primer and the second primer, both of which are from 15 to 35 nucleotides in length. The first p30 primer comprises a sequence which is complementary to a consecutive sequence, within the following sequence: CCA AGTGTCTCAC ATTTTGGTAG CTTCTCAGCT AAAGAAGAAA GCAAATCAAC TGTTGGAGTTTTTGGATTAA AACATGATTG GGATGGAAGT CCAATACTTA AGAATAAACA CGCTGACTTTACTGTTCCAA AC. SEQ ID NO.1. The second p30 primer comprises a sequence which is complementary to the inverse complement of a consecutive sequence contained within the following sequence: GTTACT CAATGGGTGG CCCAAGAATA GAATTCGAAA TATCTTATGA AGCATTCGAC GTAAAAAGTC CTAATATCAA TTATCAAAAT GACGCGCACA GGTACTGCGC TCTATCTCAT CACACATCGG CAGCCAT, SEQ ID NO.2. The first p28 comprises a sequence which is complementary to a consecutive sequenc, within the following sequence: A GTTTTCATAA CAAGTGCATT GATATCACTA ATATCTTCTC TACCTGGAGT ATCATTTTCC GACCCAACAG GTAGTGGTAT TAACGG, SEQ ID NO. 3. The second p28 primercomprises a sequence which is complementary to the inverse complement of a consecutive sequencewithin one of the following two sequences: CAT TTCTAGGTTT TGCAGGAGCT ATTGGCTACT CAATGGATGG TCCAAGAATA GAGCTTGAAG TATCTTATGA, SEQ ID NO. 4, or C AAGGAAAGTT AGGTTTAAGC TACTCTATAA GCCCAGA, SEQ ID NO. 5.

摘要翻译： 提供了用于检测从动物获得的样品中E. canis和E.Chaffeensis的存在的工具和方法。 该方法采用聚合酶链反应和引物组，其基于大肠杆菌的p30基因和E.Chaffeensis的p28基因。 本发明还涉及p30和p28引物组。 每个p30引物组包含长度为15至35个核苷酸的第一引物和第二引物。 第一个p30引物包含与以下序列中的连续序列互补的序列：CCA AGTGTCTCAC ATTTTGGTAG CTTCTCAGCT AAAGAAGAAA GCAAATCAAC TGTTGGAGTTTTTGGATTAA AACATGATTG GGATGGAAGT CCAATACTTA AGAATAAACA CGCTGACTTTACTGTTCCAA AC。 SEQ ID NO.1。 第二个p30引物包含与以下序列中包含的连续序列的反向互补序列互补的序列：GTTACT CAATGGGTGG CCCAAGAATA GAATTCGAAA TATCTTATGA AGCATTCGAC GTAAAAAGTC CTAATATCAA TTATCAAAAT GACGCGCACA GGTACTGCGC TCTATCTCAT CACACATCGG CAGCCAT，SEQ ID NO.2。 第一个p28包含与以下序列中的连续序列互补的序列：GTTTTCATAA CAAGTGCATT GATATCACTA ATATCTTCTC TACCTGGAGT ATCATTTTCC GACCCAACAG GTAGTGGTAT TAACGG，SEQ ID NO： 第二个p28引物包含与以下两个序列之一的连续序列的反向互补序列互补的序列：CAT TTCTAGGTTT TGCAGGAGCT ATTGGCTACT CAATGGATGG TCCAAGAATA GAGCTTGAAG TATCTTATGA，SEQ ID NO： 4或C AAGGAAAGTT AGGTTTAAGC TACTCTATAA GCCCAGA，SEQ ID NO： 5。