公开(公告)号：US20110284466A1

公开(公告)日：2011-11-24

申请号：US13112519

申请日：2011-05-20

申请人： Chao-Jung Chen ,  Mei-Chun Tseng ,  Yet-Ran Chen

发明人： Chao-Jung Chen ,  Mei-Chun Tseng ,  Yet-Ran Chen

IPC分类号： B01D15/08 ,  C04B35/64 ,  B05D3/00 ,  B05D7/22 ,  B05D3/02

CPC分类号： G01N30/603 ,  B01D15/22 ,  G01N30/6078

摘要： A durable on-column tunneled frit was developed for use in nanoflow UPLC systems. The frit was tunneled during sol-gel reaction. The tunneled-frit fabrication process is easy and reproducible in terms of back pressure and durability. This design creates low backpressure with high liquid flow, which is suitable for the nanoflowUPLC application. A short packing (2 cm C18 particle) tunneled-frit column was demonstrated to sustain 10,000 psi continuous liquid flow for over one week without any particle leakage or pressure instability. The tunneled-frit was also successfully applied to the fabrication of nanoUPLC trapping and analytical column system. It was demonstrated to have high separation efficiency and sensitivity for the analysis of tryptic peptides as well as improved detection sensitivity for phosphopeptide analysis.

摘要翻译： 开发了一种耐用的柱内隧道玻璃料，用于纳流低压UPLC系统。 玻璃料在溶胶 - 凝胶反应过程中被隧道化。 隧道式玻璃料制造工艺在背压和耐久性方面是容易的和可再现的。 该设计产生低液压，具有高液体流量，适用于纳米FLUP应用。 证明短填料（2cm C18颗粒）隧道玻璃料柱能够持续10,000psi连续液流超过一周，没有任何颗粒泄漏或压力不稳定性。 隧道玻璃料也成功应用于nanoUPLC捕集和分析柱系统的制造。 已被证明具有高分离效率和敏感性，用于分析胰蛋白酶肽，以及提高磷酸肽分析的检测灵敏度。

公开(公告)号：US20170332645A1

公开(公告)日：2017-11-23

申请号：US15521927

申请日：2015-10-27

申请人： Chi-Ming LIANG ,  Yet-Ran CHEN ,  Ying-Lan CHEN ,  Mei-Chun TSENG ,  Academia Sinica

发明人： Yet-Ran Chen ,  Ying-Lan Chen ,  Mei-Chun Tseng

IPC分类号： A01N65/38 ,  C07K14/415

CPC分类号： A01N65/38 ,  A61K38/00 ,  C07K14/415 ,  C12N15/8271 ,  C12N15/8279

摘要： The present invention relates to a novel plant defense signaling peptide and applications thereof.

公开(公告)号：US09267927B2

公开(公告)日：2016-02-23

申请号：US13112519

申请日：2011-05-20

申请人： Chao-Jung Chen ,  Mei-Chun Tseng ,  Yet-Ran Chen

发明人： Chao-Jung Chen ,  Mei-Chun Tseng ,  Yet-Ran Chen

IPC分类号： G01N30/60 ,  B01D15/22

CPC分类号： G01N30/603 ,  B01D15/22 ,  G01N30/6078

摘要： A durable on-column tunneled frit was developed for use in nanoflow UPLC systems. The frit was tunneled during sol-gel reaction. The tunneled-frit fabrication process is easy and reproducible in terms of back pressure and durability. This design creates low backpressure with high liquid flow, which is suitable for the nanoflowUPLC application. A short packing (2 cm C18 particle) tunneled-frit column was demonstrated to sustain 10,000 psi continuous liquid flow for over one week without any particle leakage or pressure instability. The tunneled-frit was also successfully applied to the fabrication of nanoUPLC trapping and analytical column system. It was demonstrated to have high separation efficiency and sensitivity for the analysis of tryptic peptides as well as improved detection sensitivity for phosphopeptide analysis.

摘要翻译： 开发了一种耐用的柱内隧道玻璃料，用于纳流低压UPLC系统。 玻璃料在溶胶 - 凝胶反应过程中被隧道化。 隧道式玻璃料制造工艺在背压和耐久性方面是容易的和可再现的。 该设计产生低液压，具有高液体流量，适用于纳米FLUP应用。 证明短填料（2cm C18颗粒）隧道玻璃料柱能够持续10,000psi连续液流超过一周，没有任何颗粒泄漏或压力不稳定性。 隧道玻璃料也成功应用于nanoUPLC捕集和分析柱系统的制造。 已被证明具有高分离效率和敏感性，用于分析胰蛋白酶肽，以及提高磷酸肽分析的检测灵敏度。