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    Hybrid/capture probe designs for full-length cDNA
    1.
    发明授权
    Hybrid/capture probe designs for full-length cDNA 有权

    公开(公告)号：US12168801B1

    公开(公告)日：2024-12-17

    申请号：US17367127

    申请日：2021-07-02

    Applicant: 10X Genomics, Inc.

    Inventor： Andrew John Hill ,  Katherine Pfeiffer ,  Andrew Scott Kohlway

    IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876

    Abstract: Systems and methods for identifying and using hybrid/capture probes are provided. cDNA sequences from poly-adenylated mRNA are obtained. Each cDNA sequence in a first subset maps to a gene in a plurality of genes. Each cDNA sequence in a second subset maps to a reference genome portion not represented by the plurality of genes. Each gene has a corresponding plurality of transcripts. The cDNA sequences are exposed to at least 2×103 nucleic acid baits between K1 and K2 residues long, forming nucleic acid bait—sequence read complexes. Each nucleic acid bait that hybridizes to a cDNA sequence mapping to a gene selectively hybridizes to a first subset or another subset of transcripts corresponding to the gene. Each transcript of each gene is hybridizable to a nucleic acid bait in the at least 2×103 nucleic acid baits. The nucleic acid bait—sequence read complexes are captured and analyzed.

    PROBE-BASED ANALYSIS OF NUCLEIC ACIDS AND PROTEINS
    3.
    发明公开
    PROBE-BASED ANALYSIS OF NUCLEIC ACIDS AND PROTEINS 审中-公开

    公开(公告)号：US20240002914A1

    公开(公告)日：2024-01-04

    申请号：US18217310

    申请日：2023-06-30

    Applicant: 10x Genomics, Inc.

    Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

    IPC: C12Q1/6841 ,  G01N1/30

    CPC classification number: C12Q1/6841 ,  G01N1/30

    Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

    Probe-based analysis of nucleic acids and proteins
    5.
    发明授权
    Probe-based analysis of nucleic acids and proteins 有权

    公开(公告)号：US11952626B2

    公开(公告)日：2024-04-09

    申请号：US18236540

    申请日：2023-08-22

    Applicant: 10x Genomics, Inc.

    Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6874

    CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

    Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

    PROBE-BASED ANALYSIS OF NUCLEIC ACIDS AND PROTEINS
    6.
    发明公开
    PROBE-BASED ANALYSIS OF NUCLEIC ACIDS AND PROTEINS 审中-公开

    公开(公告)号：US20230399691A1

    公开(公告)日：2023-12-14

    申请号：US18236540

    申请日：2023-08-22

    Applicant: 10x Genomics, Inc.

    Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund

    IPC: C12Q1/6874 ,  C12Q1/6818

    CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

    Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

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