公开(公告)号：US12084715B1

公开(公告)日：2024-09-10

申请号：US17518213

申请日：2021-11-03

Applicant: 10x Genomics, Inc.

Inventor： Paul Eugene Lund

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6848

CPC classification number: C12Q1/6848 ,  C12Q1/6806

Abstract: Disclosed herein is a method of preventing the amplification of artifactual antisense products when sequencing a target polynucleotide. Incorporation of modified nucleotides into handle polynucleotide sequences can allow for the selective degradation of artifact antisense products generated by mis-priming, prior to amplification, thus preventing them from appearing in the amplification library. Selective degradation can be accomplished using one or more agents configured to specifically cleave the handle polynucleotide at the modified nucleotide. Amplification can be performed using a primer that is configured to hybridize to the handle sequence and a second primer that is configured to hybridize to a complement of a target nucleotide.

公开(公告)号：US20230279481A1

公开(公告)日：2023-09-07

申请号：US18196835

申请日：2023-05-12

Applicant: 10x Genomics, Inc.

Inventor： Sean Marrache ,  Shalini Gohil ,  Joshua Delaney ,  Paul Eugene Lund

IPC: C12Q1/6855

CPC classification number: C12Q1/6855

Abstract: The present disclosure provides compositions, methods, and kits comprising a nano-partition contained within a partition that allows for the separation and use of at least two enzymes in a partition-based assay of a biological sample, such as a single cell in a droplet. The compositions and methods are useful for carrying out partition-based assays of fixed biological samples.

公开(公告)号：US20240019422A1

公开(公告)日：2024-01-18

申请号：US18370826

申请日：2023-09-20

Applicant: 10X GENOMICS, INC.

Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/532 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B30/04

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240002914A1

公开(公告)日：2024-01-04

申请号：US18217310

申请日：2023-06-30

Applicant: 10x Genomics, Inc.

Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: C12Q1/6841 ,  G01N1/30

CPC classification number: C12Q1/6841 ,  G01N1/30

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240254552A1

公开(公告)日：2024-08-01

申请号：US18412111

申请日：2024-01-12

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: C12Q1/6874 ,  C12Q1/6818

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US11952626B2

公开(公告)日：2024-04-09

申请号：US18236540

申请日：2023-08-22

Applicant: 10x Genomics, Inc.

Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20230399691A1

公开(公告)日：2023-12-14

申请号：US18236540

申请日：2023-08-22

Applicant: 10x Genomics, Inc.

Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund

IPC: C12Q1/6874 ,  C12Q1/6818

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.