公开(公告)号：US20150140577A1

公开(公告)日：2015-05-21

申请号：US14547963

申请日：2014-11-19

申请人： ACEA Biosciences, Inc.

发明人： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC分类号： G01N15/14 ,  G01N33/569 ,  G01N21/64

CPC分类号： G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

摘要： An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

摘要翻译： 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组的光束整形光学器件，其中每个组件包括两个透镜，用于可调节地将光沿x轴水平地聚焦到相同的水平位置，并且沿着y轴沿着同一平面垂直地垂直于不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，根据波长范围将从流通池收集的荧光过滤到不同的检测通道; 以及用于将滤波后的荧光转换为电信号的每个检测通道的检测器，其中对电信号进行处理，使得来自不同垂直位置处的每个激光的荧光在相同的检测器处被区分。

公开(公告)号：US20130200277A1

公开(公告)日：2013-08-08

申请号：US13657845

申请日：2012-10-22

申请人： ACEA BIOSCIENCES, INC

发明人： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

摘要： A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

摘要翻译： 一种用于检测由多个激发源引起的光的信号分量的系统，包括：流路，包括至少两个空间分离的光询问区; 非调制激励源，其将第一波长的光束以接近恒定的强度引导到第一光学询问区上; 调制激励源，其将以调制频率随时间调制的第二波长的光束引导到第二光学询问区上; 检测器子系统，包括一组检测器，其被配置为检测从流过所述至少两个光询问区的颗粒发射的光并将检测到的光转换成总电信号; 以及处理器，其根据从每个光询问区检测的光来确定信号分量。

公开(公告)号：US20130100443A1

公开(公告)日：2013-04-25

申请号：US13656457

申请日：2012-10-19

申请人： ACEA BIOSCIENCES, INC

发明人： Nan Li ,  Ye Chen ,  Xiaobo Wang

IPC分类号： G02B27/10 ,  G01J3/443

CPC分类号： G02B27/1006 ,  G01J3/14 ,  G01J3/36 ,  G01J3/443 ,  G02B5/04 ,  G02B5/285 ,  G02B27/141 ,  G02B27/142

摘要： A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.

摘要翻译： 一种棱镜，包括面以提供多个平坦表面的基板，其中，所述多个表面中的至少两个表面，每个包括过滤器涂层，形成至少两个过滤器表面，其中每个过滤器表面选择性地允许预定波长的通过并反射 沿着光路的剩余波长朝向多个表面中的另一个，可选地另外的滤光器表面，其中沿着光路的多个表面中的每一个的入射角相等或接近相等。 一种结合棱镜的装置及其用于将光谱分解成多个波长或波长范围的用途。

公开(公告)号：US10234392B2

公开(公告)日：2019-03-19

申请号：US15416976

申请日：2017-01-26

申请人： ACEA Biosciences, Inc.

发明人： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC分类号： G01N21/64 ,  G01N33/569 ,  G01N15/14 ,  G01N15/10

摘要： An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

公开(公告)号：US10648913B2

公开(公告)日：2020-05-12

申请号：US15415439

申请日：2017-01-25

申请人： ACEA BIOSCIENCES, INC

发明人： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC分类号： G01N21/64 ,  G01N15/14 ,  G01N15/10

摘要： A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：US20170138856A1

公开(公告)日：2017-05-18

申请号：US15416976

申请日：2017-01-26

申请人： ACEA Biosciences, Inc.

发明人： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC分类号： G01N21/64 ,  G01N15/14

CPC分类号： G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

摘要： An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

公开(公告)号：US20170131206A1

公开(公告)日：2017-05-11

申请号：US15415439

申请日：2017-01-25

申请人： ACEA BIOSCIENCES, INC

发明人： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC分类号： G01N21/64 ,  G01N15/14

CPC分类号： G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

摘要： A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：US09568423B2

公开(公告)日：2017-02-14

申请号：US13657845

申请日：2012-10-22

申请人： ACEA BIOSCIENCES, INC

发明人： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC分类号： G01N21/64 ,  G01F1/00 ,  G01N15/14 ,  G01N15/10

CPC分类号： G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

摘要： A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

摘要翻译： 一种用于检测由多个激发源引起的光的信号分量的系统，包括：流路，包括至少两个空间分离的光询问区; 非调制激励源，其将第一波长的光束以接近恒定的强度引导到第一光学询问区上; 调制激励源，其将以调制频率随时间调制的第二波长的光束引导到第二光学询问区上; 检测器子系统，包括一组检测器，其被配置为检测从流过所述至少两个光询问区的颗粒发射的光并将检测到的光转换成总电信号; 以及处理器，其根据从每个光询问区检测的光来确定信号分量。

公开(公告)号：US20200292454A1

公开(公告)日：2020-09-17

申请号：US16833235

申请日：2020-03-27

申请人： ACEA Biosciences, Inc.

发明人： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC分类号： G01N21/64 ,  G01N15/14

摘要： A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：US09575063B2

公开(公告)日：2017-02-21

申请号：US14547963

申请日：2014-11-19

申请人： ACEA Biosciences, Inc.

发明人： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC分类号： G01N21/00 ,  G01N33/569 ,  G01N15/14 ,  G01N15/10

CPC分类号： G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

摘要： An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

摘要翻译： 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组的光束整形光学器件，其中每个组件包括两个透镜，用于可调节地将光沿x轴水平地聚焦到相同的水平位置，并且沿着y轴沿着同一平面垂直地垂直于不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，根据波长范围将从流通池收集的荧光过滤到不同的检测通道; 以及用于将滤波后的荧光转换为电信号的每个检测通道的检测器，其中对电信号进行处理，使得来自不同垂直位置处的每个激光的荧光在相同的检测器处被区分。