公开(公告)号：US10689622B2

公开(公告)日：2020-06-23

申请号：US15847246

申请日：2017-12-19

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Nao Sugimoto ,  Manabu Kitazawa ,  Satoru Okamoto ,  Sho Senda ,  Ikue Harata ,  Satoru Ohashi

IPC: C12N5/0775 ,  C12N5/074 ,  C08G65/48 ,  C08G63/91

Abstract: A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.

公开(公告)号：US10745669B2

公开(公告)日：2020-08-18

申请号：US14813374

申请日：2015-07-30

Applicant: AJINOMOTO CO., INC.

Inventor： Sho Senda ,  Tomomi Yoshida ,  Satoru Okamoto ,  Yoko Kuriyama ,  Manabu Kitazawa ,  Ikue Harata ,  Nao Sugimoto

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/074 ,  C12N5/0735

Abstract: Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

公开(公告)号：US09890359B2

公开(公告)日：2018-02-13

申请号：US14497926

申请日：2014-09-26

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Nao Sugimoto ,  Manabu Kitazawa ,  Satoru Okamoto ,  Sho Senda ,  Ikue Harata ,  Satoru Ohashi

IPC: C12N5/0775 ,  C12N5/074 ,  C08G63/91 ,  C08G65/48

CPC classification number: C12N5/0663 ,  C08G63/912 ,  C08G65/48 ,  C12N5/0696 ,  C12N2500/34 ,  C12N2501/115

Abstract: A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.

公开(公告)号：US10947508B2

公开(公告)日：2021-03-16

申请号：US15435705

申请日：2017-02-17

Applicant: AJINOMOTO CO., INC. ,  Kyoto University

Inventor： Yonghui Jin ,  Ikue Harata ,  Sho Senda ,  Hiroki Ozawa ,  Tsuyoshi Kobayashi ,  Junya Toguchida ,  Makoto Ikeya

IPC: C12N5/0775 ,  C12N5/00 ,  A61K35/55 ,  A61K35/56 ,  A61K35/28 ,  A61K35/12

Abstract: Mesenchymal stem cells may be culture for a long period, without using any special apparatus, equipment and the like, in a medium in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid are reduced.

公开(公告)号：US10662411B2

公开(公告)日：2020-05-26

申请号：US16242463

申请日：2019-01-08

Applicant: AJINOMOTO CO., INC.

Inventor： Sho Senda ,  Tomomi Yoshida ,  Satoru Okamoto ,  Yoko Kuriyama ,  Manabu Kitazawa ,  Ikue Harata ,  Nao Sugimoto

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/074 ,  C12N5/0735

Abstract: Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

公开(公告)号：US20180105798A1

公开(公告)日：2018-04-19

申请号：US15847246

申请日：2017-12-19

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko KURIYAMA ,  Nao Sugimoto ,  Manabu Kitazawa ,  Satoru Okamoto ,  Sho Senda ,  Ikue Harata ,  Satoru Ohashi

IPC: C12N5/0775 ,  C08G65/48 ,  C08G63/91 ,  C12N5/074

Abstract: A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.