公开(公告)号：US10689622B2

公开(公告)日：2020-06-23

申请号：US15847246

申请日：2017-12-19

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Nao Sugimoto ,  Manabu Kitazawa ,  Satoru Okamoto ,  Sho Senda ,  Ikue Harata ,  Satoru Ohashi

IPC: C12N5/0775 ,  C12N5/074 ,  C08G65/48 ,  C08G63/91

Abstract: A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.

公开(公告)号：US10745669B2

公开(公告)日：2020-08-18

申请号：US14813374

申请日：2015-07-30

Applicant: AJINOMOTO CO., INC.

Inventor： Sho Senda ,  Tomomi Yoshida ,  Satoru Okamoto ,  Yoko Kuriyama ,  Manabu Kitazawa ,  Ikue Harata ,  Nao Sugimoto

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/074 ,  C12N5/0735

Abstract: Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

公开(公告)号：US10662411B2

公开(公告)日：2020-05-26

申请号：US16242463

申请日：2019-01-08

Applicant: AJINOMOTO CO., INC.

Inventor： Sho Senda ,  Tomomi Yoshida ,  Satoru Okamoto ,  Yoko Kuriyama ,  Manabu Kitazawa ,  Ikue Harata ,  Nao Sugimoto

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/074 ,  C12N5/0735

Abstract: Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

公开(公告)号：US11859205B2

公开(公告)日：2024-01-02

申请号：US16898844

申请日：2020-06-11

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Sho Senda ,  Yumi Ando ,  Tomomi Yoshida ,  Haruna Sato ,  Daisuke Ejima ,  Takayoshi Fujii ,  Masayo Date ,  Manabu Kitazawa

IPC: C12N5/074 ,  C12N5/0735 ,  C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0602 ,  C12N5/0018 ,  C12N5/0696 ,  C12N5/0606 ,  C12N2500/36 ,  C12N2501/998

Abstract: Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.

公开(公告)号：US10457911B2

公开(公告)日：2019-10-29

申请号：US15275586

申请日：2016-09-26

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Satoru Okamoto ,  Manabu Kitazawa ,  Nao Sugimoto ,  Takuya Matsumoto

IPC: C12N5/00 ,  C12N5/074

Abstract: Culture media which contain a water-soluble polymer, such as polyvinyl alcohol and the like, and albumin, and the like, are useful for the proliferation of stem cells, and show good culture results, particularly with iPS cells, and maintain the stability of the medium properties, as well as using a reduced amount of albumin.

公开(公告)号：US09890359B2

公开(公告)日：2018-02-13

申请号：US14497926

申请日：2014-09-26

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Nao Sugimoto ,  Manabu Kitazawa ,  Satoru Okamoto ,  Sho Senda ,  Ikue Harata ,  Satoru Ohashi

IPC: C12N5/0775 ,  C12N5/074 ,  C08G63/91 ,  C08G65/48

CPC classification number: C12N5/0663 ,  C08G63/912 ,  C08G65/48 ,  C12N5/0696 ,  C12N2500/34 ,  C12N2501/115

Abstract: A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.