公开(公告)号：US11859205B2

公开(公告)日：2024-01-02

申请号：US16898844

申请日：2020-06-11

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Kuriyama ,  Sho Senda ,  Yumi Ando ,  Tomomi Yoshida ,  Haruna Sato ,  Daisuke Ejima ,  Takayoshi Fujii ,  Masayo Date ,  Manabu Kitazawa

IPC: C12N5/074 ,  C12N5/0735 ,  C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0602 ,  C12N5/0018 ,  C12N5/0696 ,  C12N5/0606 ,  C12N2500/36 ,  C12N2501/998

Abstract: Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.

公开(公告)号：US20140349371A1

公开(公告)日：2014-11-27

申请号：US14455068

申请日：2014-08-08

Applicant: AJINOMOTO CO., INC.

Inventor： Ryosuke Yumioka ,  Daisuke Ejima

IPC: C07K14/54 ,  C07K16/44 ,  C07K16/18 ,  C12N9/10

CPC classification number: C07K14/5412 ,  C07K1/1136 ,  C07K1/145 ,  C07K14/475 ,  C07K16/18 ,  C07K16/44 ,  C07K2317/569 ,  C07K2317/64 ,  C12N9/104 ,  C12N9/1044

Abstract: The present invention provides a method for producing a protein which has a restored native higher-order structure by bringing a protein which has lost its native higher-order structure into contact at pH 6.5 to 9.0 with a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic acid to obtain a solubilized solution of the protein; and then adding the solubilized solution to a buffer with pH 6.5 to 9.0 containing arginine or an arginine derivative at a concentration of 0.1 to 1.2 M to lower the concentration of the specific surfactant, such as lauroylglutamic acid, in the obtained mixture solution down to 0.02 to 0.275%. According to the present invention, it is possible to easily restore the native higher-order structure of a protein while smoothly removing the surfactant from the protein.

Abstract translation: 本发明提供一种生产蛋白质的方法，该蛋白质具有恢复的天然高级结构，通过使具有1至3％的特异性的1％至3％的水溶液使具有失去其天然高级结构的蛋白质在pH 6.5至9.0下接触 表面活性剂如月桂酰谷氨酸以获得蛋白质的溶解溶液; 然后将溶解的溶液加入到含有浓度为0.1至1.2M的精氨酸或精氨酸衍生物的pH 6.5至9.0的缓冲液中，以将得到的混合溶液中的特定表面活性剂如月桂酰谷氨酸的浓度降低至0.02 至0.275％。 根据本发明，可以容易地恢复蛋白质的天然高级结构，同时平滑地从蛋白质中除去表面活性剂。

公开(公告)号：US09462810B2

公开(公告)日：2016-10-11

申请号：US14108502

申请日：2013-12-17

Applicant: AJINOMOTO CO., INC.

Inventor： Hajime Koyama ,  Tsutomu Arakawa ,  Daisuke Ejima

IPC: C12N7/06 ,  C12N7/00 ,  A61K39/21 ,  A01N47/44 ,  C12N7/04 ,  A23L3/3508 ,  A61K31/198 ,  A61K39/00

CPC classification number: A01N47/44 ,  A23L3/3508 ,  A23V2002/00 ,  A61K31/198 ,  A61K2039/5252 ,  C12N7/00 ,  C12N7/04 ,  C12N7/06 ,  C12N2710/16663 ,  C12N2760/16163 ,  C12N2760/18863 ,  A23V2200/10 ,  A23V2250/0606

Abstract: The present invention provides a method for conveniently producing a protein formulation in which viruses are inactivated, without impairing the quality of the obtained protein formulation, characterized by including the step of exposing the protein formulation contaminated with the viruses to a 0.1-2M aqueous solution of arginine, an arginine derivative, or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5. The present invention also provides a virus inactivation method characterized by including the step of contacting a virus-containing object with a 0.1-2M aqueous solution of arginine, an arginine derivative, or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5.

Abstract translation: 本发明提供了一种方便地制备其中病毒灭活而不损害所获得的蛋白质制剂的质量的蛋白质制剂的方法，其特征在于包括将被病毒污染的蛋白质制剂暴露于0.1-2M的 精氨酸，精氨酸衍生物或其混合物，将该水溶液调节至pH 3.5至5.本发明还提供了一种病毒灭活方法，其特征在于包括使病毒含有物体与0.1-2M水溶液接触的步骤 的精氨酸，精氨酸衍生物或其混合物，将该水溶液调节至pH 3.5至5。

公开(公告)号：US20140107207A1

公开(公告)日：2014-04-17

申请号：US14108502

申请日：2013-12-17

Applicant: AJINOMOTO CO., INC.

Inventor： Hajime Koyama ,  Tsutomu Arakawa ,  Daisuke Ejima

IPC: A01N47/44 ,  A61K31/198

CPC classification number: A01N47/44 ,  A23L3/3508 ,  A23V2002/00 ,  A61K31/198 ,  A61K2039/5252 ,  C12N7/00 ,  C12N7/04 ,  C12N7/06 ,  C12N2710/16663 ,  C12N2760/16163 ,  C12N2760/18863 ,  A23V2200/10 ,  A23V2250/0606

Abstract: The present invention provides a method for conveniently producing a protein formulation in which viruses are inactivated, without impairing the quality of the obtained protein formulation, characterized by including the step of exposing the protein formulation contaminated with the viruses to a 0.1-2M aqueous solution of arginine, an arginine derivative, or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5. The present invention also provides a virus inactivation method characterized by including the step of contacting a virus-containing object with a 0.1-2M aqueous solution of arginine, an arginine derivative, or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5.

Abstract translation: 本发明提供了一种方便地制备其中病毒灭活而不损害所获得的蛋白质制剂的质量的蛋白质制剂的方法，其特征在于包括将被病毒污染的蛋白质制剂暴露于0.1-2M的 精氨酸，精氨酸衍生物或其混合物，将该水溶液调节至pH 3.5至5.本发明还提供了一种病毒灭活方法，其特征在于包括使病毒含有物体与0.1-2M水溶液接触的步骤 的精氨酸，精氨酸衍生物或其混合物，将该水溶液调节至pH 3.5至5。

公开(公告)号：US20140099672A1

公开(公告)日：2014-04-10

申请号：US14104139

申请日：2013-12-12

Applicant: Ajinomoto Co., Inc.

Inventor： Daisuke Ejima ,  Haruna Sato ,  Kouhei Tsumoto ,  Masayo Date

IPC: C12P21/00

CPC classification number: C12P21/00 ,  C07K1/1136 ,  C07K14/36 ,  C07K16/40 ,  C07K2317/622 ,  C07K2319/00 ,  C12P21/02

Abstract: The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.

Abstract translation: 本发明提供一种由单体蛋白质组成的多聚体蛋白质的制造方法，其特征在于，通过将具有免疫球蛋白折叠结构的蛋白质与能够作为亚单位结构的蛋白质融合而得到的单体蛋白质，其特征在于，包括以下步骤： （A）在微生物的细胞中制备具有不溶性颗粒形式的单体蛋白质; （B）用含有月桂酰-L-Glu的水溶液使步骤（A）中制备的单体蛋白质溶解; （C）在含有精氨酸盐酸盐的缓冲液中稀释步骤（B）中得到的溶液以降低月桂酰-L-Glu的浓度; 和（D）使用凝胶过滤色谱法等用缓冲液代替步骤（C）中得到的溶液的溶剂。

公开(公告)号：US10308969B2

公开(公告)日：2019-06-04

申请号：US14104139

申请日：2013-12-12

Applicant: AJINOMOTO CO., INC.

Inventor： Daisuke Ejima ,  Haruna Sato ,  Kouhei Tsumoto ,  Masayo Date

IPC: C12P21/00 ,  C07K14/36 ,  C07K16/40 ,  C07K1/113 ,  C12P21/02

Abstract: The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.