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公开(公告)号：US10583397B2
公开(公告)日：2020-03-10
申请号：US15302762
申请日：2015-05-13
Applicant: AMGEN INC.
Inventor： Eva Gefroh , Randolph W. Schweickart , Krista Petty , Gregory Frank , Christine Salstrom Terpsma , Arthur C. Hewig, III , Joseph Edward Shultz
Abstract: Systems and methods used to control tangential flow filtration are provided, including control systems and methods for use with connected systems with upstream processing units, such as chromatography processing units, in fluid communication with a tangential flow filtration processing unit. Also included are control systems and methods for performing continuous concentration using single-pass tangential flow filtration with permeate flow control.
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公开(公告)号：US09856287B2
公开(公告)日：2018-01-02
申请号：US15422327
申请日：2017-02-01
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart , Ronald Nixon Keener, III
CPC classification number: C07K1/1136 , C07K1/1133 , C07K1/14 , C07K14/00 , C07K16/00 , C07K2319/30
Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
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公开(公告)号：US20200147550A1
公开(公告)日：2020-05-14
申请号：US16743728
申请日：2020-01-15
Applicant: AMGEN INC.
Inventor： Eva Gefroh , Randolph W. Schweickart , Krista Petty , Gregory Frank , Christine Salstrom Terpsma , Arthur C, Hewig, III , Joseph Edward Shultz
Abstract: Systems and methods used to control tangential flow filtration are provided, including control systems and methods for use with connected systems with upstream processing units, such as chromatography processing units, in fluid communication with a tangential flow filtration processing unit. Also included are control systems and methods for performing continuous concentration using single-pass tangential flow filtration with permeate flow control.
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公开(公告)号：US20170157566A1
公开(公告)日：2017-06-08
申请号：US15302762
申请日：2015-05-13
Applicant: AMGEN INC.
Inventor： Eva Gefroh , Randolph W. Schweickart , Krista Petty , Gregory Frank , Christine Salstrom Terpsma , Arthur C. Hewig, III , Joseph Edward Shultz
Abstract: Systems and methods used to control tangential flow filtration are provided, including control systems and methods for use with connected systems with upstream processing units, such as chromatography processing units, in fluid communication with a tangential flow filtration processing unit. Also included are control systems and methods for performing continuous concentration using single-pass tangential flow filtration with permeate flow control.
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公开(公告)号：US12269843B2
公开(公告)日：2025-04-08
申请号：US15889559
申请日：2018-02-06
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart , Ronald Nixon Keener, III
IPC: A61K39/395 , C07K1/113 , C07K1/14 , C07K14/00 , C07K16/00
Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
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Patent Agency Ranking
公开(公告)号：US11786866B2
公开(公告)日：2023-10-17
申请号：US17362282
申请日：2021-06-29
Applicant: AMGEN INC.
Inventor： Eva Gefroh , Randolph W. Schweickart , Krista Petty , Gregory Frank , Christine Salstrom Terpsma , Arthur C. Hewig, III , Joseph Edward Shultz
CPC classification number: B01D61/22 , B01D61/145 , B01D61/146 , B01D61/18 , C07K1/34 , B01D2311/04 , B01D2311/06 , B01D2313/18 , B01D2313/243 , B01D2315/10 , B01D2311/04 , B01D2311/2626 , B01D2311/2649 , B01D2311/06 , B01D2311/16
Abstract: Systems and methods used to control tangential flow filtration are provided, including control systems and methods for use with connected systems with upstream processing units, such as chromatography processing units, in fluid communication with a tangential flow filtration processing unit. Also included are control systems and methods for performing continuous concentration using single-pass tangential flow filtration with permeate flow control.
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公开(公告)号：US10577392B2
公开(公告)日：2020-03-03
申请号：US15476691
申请日：2017-03-31
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart
Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
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公开(公告)号：US09643997B2
公开(公告)日：2017-05-09
申请号：US14599336
申请日：2015-01-16
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart
Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
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公开(公告)号：US11407784B2
公开(公告)日：2022-08-09
申请号：US16709491
申请日：2019-12-10
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart
Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
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公开(公告)号：US20210339196A1
公开(公告)日：2021-11-04
申请号：US17362282
申请日：2021-06-29
Applicant: AMGEN INC.
Inventor： Eva Gefroh , Randolph W. Schweickart , Krista Petty , Gregory Frank , Christine Salstrom Terpsma , Arthur C. Hewig, III , Joseph Edward Shultz
Abstract: Systems and methods used to control tangential flow filtration are provided, including control systems and methods for use with connected systems with upstream processing units, such as chromatography processing units, in fluid communication with a tangential flow filtration processing unit. Also included are control systems and methods for performing continuous concentration using single-pass tangential flow filtration with permeate flow control.
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