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公开(公告)号：US20230148020A1
公开(公告)日：2023-05-11
申请号：US17920668
申请日：2021-04-21
Applicant: AMGEN INC.
Inventor： Or Dan , Roger Hart , Hann-Chung Wong
CPC classification number: G01N30/8693 , G01N30/8662 , B01D15/362 , B01D15/34 , B01D15/3809 , C07K1/16
Abstract: In a method for facilitating selection of chromatography parameters for manufacturing a therapeutic protein, one or more process parameter values associated with a hypothetical chromatography process, and one or more molecular descriptors descriptive of the therapeutic protein, are received. The method also includes predicting a performance indicator for the hypothetical chromatography process at least by analyzing the one or more process parameters and the one or more molecular descriptors using a machine learning model. The machine learning model is a regression tree model, an extreme gradient boost model, or an elastic net model. The method also includes causing the predicted performance indicator, and/or an indication of whether the predicted performance indicator satisfies one or more acceptability criteria, to be presented to a user via a user interface.
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公开(公告)号：US09856287B2
公开(公告)日：2018-01-02
申请号：US15422327
申请日：2017-02-01
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart , Ronald Nixon Keener, III
CPC classification number: C07K1/1136 , C07K1/1133 , C07K1/14 , C07K14/00 , C07K16/00 , C07K2319/30
Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
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公开(公告)号：US11407784B2
公开(公告)日：2022-08-09
申请号：US16709491
申请日：2019-12-10
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart
Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
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4.发明申请METHODS OF REDUCING OR ELIMINATING PROTEIN MODIFICATION AND DEGRADATION ARISING FROM EXPOSURE TO UV LIGHT 审中-公开减少或消除从接触到紫外线的蛋白质修饰和降解的方法公开(公告)号：US20140329227A1
公开(公告)日：2014-11-06
申请号：US14353704
申请日：2012-10-25
Applicant: Amgen Inc.
Inventor： Joseph Edward Ssultz , Roger Hart , Brent Welborn
IPC: G01N1/30
Abstract: Methods of inactivation of a virus in a sample comprising a protein component are provided. Also provided are methods of reducing protein degradation or modification in to the presence of a reactive species, such as a reactive species generated as a result of UV exposure, are also provided. In another aspect, a method of reducing oxidation of methionine residues, tryptophan residues or both methionine and tryptophan residues in a protein subjected to UV light is provided. The disclosed methods can be performed at any scale and can be automated as desired.
Abstract translation: 提供了包含蛋白质组分的样品中病毒失活的方法。 还提供了减少蛋白质降解或修饰反应物质（例如由于UV暴露产生的反应性物质）的存在的方法。 另一方面，提供了在经受紫外光的蛋白质中降低蛋氨酸残基，色氨酸残基或甲硫氨酸和色氨酸残基二氧化的方法。 所公开的方法可以以任何规模执行，并且可以根据需要自动化。
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Patent Agency Ranking
公开(公告)号：US12269843B2
公开(公告)日：2025-04-08
申请号：US15889559
申请日：2018-02-06
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart , Ronald Nixon Keener, III
IPC: A61K39/395 , C07K1/113 , C07K1/14 , C07K14/00 , C07K16/00
Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
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公开(公告)号：US10955291B2
公开(公告)日：2021-03-23
申请号：US14385249
申请日：2013-03-07
Applicant: Amgen Inc.
Inventor： Roger Hart , Amanda Lewis , Brent Welborn
Abstract: Methods of determining the dose of UVC light delivered to a sample comprising a low optical transmission complex fluid are provided. Also provided are methods of inactivation of an organism, such as a spore, a bacteria or a virus, in a sample comprising dose of UVC light delivered to a sample comprising a low optical transmission complex fluid.
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公开(公告)号：US20150053546A1
公开(公告)日：2015-02-26
申请号：US14385249
申请日：2013-03-07
Applicant: Amgen Inc.
Inventor： Roger Hart , Amanda Lewis , Brent Welborn
CPC classification number: G01J1/429 , A23L3/28 , A61L2/0035 , A61L2/0047 , A61L2/10 , A61L2202/21 , G01N21/6486 , G01N33/52 , G01N2201/06113
Abstract: Methods of determining the dose of UVC light delivered to a sample comprising a low optical transmission complex fluid are provided. Also provided are methods of inactivation of an organism, such as a spore, a bacteria or a virus, in a sample comprising dose of UVC light delivered to a sample comprising a low optical transmission complex fluid.
Abstract translation: 提供了确定输送到包含低透光复合流体的样品的UVC光的剂量的方法。 还提供了在包含剂量的UVC光的样品中灭活生物体如孢子，细菌或病毒的方法，所述UVC光递送到包含低透光复合流体的样品。
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公开(公告)号：US10577392B2
公开(公告)日：2020-03-03
申请号：US15476691
申请日：2017-03-31
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart
Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
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公开(公告)号：US09643997B2
公开(公告)日：2017-05-09
申请号：US14599336
申请日：2015-01-16
Applicant: AMGEN INC.
Inventor： Joseph Edward Shultz , Roger Hart
Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
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