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公开(公告)号：US20200165651A1
公开(公告)日：2020-05-28
申请号：US16637188
申请日：2018-08-10
申请人： AMYRIS, INC.
摘要： Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Pisum sativum kaurene oxidase or its variant kaurene oxidase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
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公开(公告)号：US11091743B2
公开(公告)日：2021-08-17
申请号：US16323756
申请日：2017-08-11
申请人： AMYRIS, INC.
发明人： Lishan Zhao , Wenzong Li , Gale Wichmann , Aditi Khankhoje , Chantal Garcia De Gonzalo , Tina Mahatdejkul-Meadows , Shaina Jackson , Michael Leavell , Darren Platt
摘要： Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
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