公开(公告)号：US11866738B2

公开(公告)日：2024-01-09

申请号：US17382963

申请日：2021-07-22

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US10519475B1

公开(公告)日：2019-12-31

申请号：US16198545

申请日：2018-11-21

Applicant: Amyris, Inc.

Inventor： Jessica Walter ,  Joshua A. Lerman ,  Michael Leavell ,  Benjamin Yap

IPC: C12P19/12 ,  C12N15/81 ,  C07H13/04 ,  C12N9/10 ,  C12N9/24 ,  C12N9/12 ,  C12N9/88

Abstract: Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides. The yeast cells include one or more heterologous nucleic acids that encode enzymes of a human milk oligosaccharide biosynthetic pathway. The yeast cells do not include a heterologous nucleic acid encoding a fucokinase. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering human milk oligosaccharides generated by the yeast cells.

公开(公告)号：US11091743B2

公开(公告)日：2021-08-17

申请号：US16323756

申请日：2017-08-11

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.