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公开(公告)号：US12104168B2
公开(公告)日：2024-10-01
申请号：US17054108
申请日：2019-05-10
Applicant: Agency For Science, Technology And Research
Inventor： Iwona Szczerbinska , Yun Shen Chan , Huck Hui Ng
CPC classification number: C12N5/0018 , C12N5/0672 , C12N5/0678 , C12N5/068 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/12 , C12N2501/13 , C12N2501/135 , C12N2501/16 , C12N2501/165 , C12N2501/727
Abstract: The present invention relates to a cell culture medium for preparing liver, gastric, pancreatic, colon or intestinal adult stem cell isolated from adult tissue, as well as for maintaining such stem cell in the undifferentiated state. The cell culture medium comprises a base medium; an ABL and SRC dual kinase inhibitor/an ABL kinase inhibitor and a SRC kinase inhibitor; a mitogenic factor; a WNT signalling pathway activator; a stimulator for NAD+ and NADP+ generation; and a cAMP/PKA pathway activator. In a particular embodiment, the ABL and SRC dual kinase inhibitor is Dasatinib; the mitogenic factor is EGF; the WNT signalling pathway activator is R-Spondin 1; the stimulator for NAD+ and NADP+ generation is nicotinamide; and the cAMP/PKA pathway activator is cholera endotoxin.
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Patent Agency Ranking
2.发明授权公开(公告)号：US09963682B2
公开(公告)日：2018-05-08
申请号：US15194130
申请日：2016-06-27
Applicant: Agency for Science, Technology and Research
Inventor： Dominic Jian-Chien Heng , Huck Hui Ng
IPC: A61K35/545 , A61K35/12 , C12N15/85 , C12N5/074
CPC classification number: C12N5/0696 , C12N15/85 , C12N2501/38 , C12N2501/602 , C12N2501/604 , C12N2501/606 , C12N2510/00
Abstract: According to the invention there is provided methods for inducing pluripotent stem cells in vitro, vectors and compositions for producing the same and methods for using the induced pluripotent stem cell for treating a patient in need of a pluripotent stem cell treatment.
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3.发明申请NUCLEAR RECEPTOR AND MUTANT THEREOF AND THE USE OF THE SAME IN THE REPROGRAMMING OF CELLS 有权核受体及其突变体及其在细胞重编中的应用公开(公告)号：US20160376559A1
公开(公告)日：2016-12-29
申请号：US15194130
申请日：2016-06-27
Applicant: Agency for Science, Technology and Research
Inventor： Dominic Jian-Chien Heng , Huck Hui Ng
CPC classification number: C12N5/0696 , C12N15/85 , C12N2501/38 , C12N2501/602 , C12N2501/604 , C12N2501/606 , C12N2510/00
Abstract: According to the invention there is provided methods for inducing pluripotent stem cells in vitro, vectors and compositions for producing the same and methods for using the induced pluripotent stem cell for treating a patient in need of a pluripotent stem cell treatment.
Abstract translation: 根据本发明，提供了用于在体外诱导多能干细胞的方法，用于制备多能干细胞的载体和组合物以及使用诱导的多能干细胞治疗需要多能干细胞治疗的患者的方法。
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公开(公告)号：US11795436B2
公开(公告)日：2023-10-24
申请号：US16865820
申请日：2020-05-04
Inventor： Yuanyu Hu , Huck Hui Ng , Yock Young Dan
IPC: C12N5/071 , A61K35/407 , C12N5/00 , A61P1/16
CPC classification number: C12N5/0672 , A61K35/407 , A61P1/16 , C12N5/0037 , C12N5/067 , C12N5/0679 , C12N2501/01 , C12N2501/11 , C12N2501/395 , C12N2501/415 , C12N2501/42
Abstract: This application describes liver stem cells (LSC), and differentiated hepatocytes, cholangiocytes, and 3D cellular structures derived therefrom. Methods for producing LSC and mature, differentiated hepatocytes and cholangiocytes in culture are provided. Also provided are cell culture systems and cell culture media for producing a homogenous population of liver stem cells that remain in an undifferentiated state over multiple passages in culture. The LSC and methods are useful for producing homogenous populations of hepatocytes and cholangiocytes for downstream applications. The LSC can be transplanted into subjects to treat liver diseases.
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公开(公告)号：US10087416B2
公开(公告)日：2018-10-02
申请号：US15031614
申请日：2014-10-27
Applicant: Agency for Science, Technology and Research
Inventor： Yun Shen Chan , Huck Hui Ng
IPC: C12N5/0735
Abstract: A method for culturing and maintaining a pluripotent stem cell in an undifferentiated state is provided. The method comprises culturing the pluripotent stem cell in a medium comprising an MEK inhibitor, a GSK3 inhibitor, a dual inhibitor of AMPK and/or BMP signaling and LIF. A cell produced by the method, cell culture medium and a kit for performing the method described is also provided.
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公开(公告)号：US10683484B2
公开(公告)日：2020-06-16
申请号：US15735321
申请日：2016-06-10
Inventor： Yuanyu Hu , Huck Hui Ng , Yock Young Dan
IPC: C12N5/071 , A61K35/407 , C12N5/00 , A61P1/16
Abstract: This application describes liver stem cells (LSC), and differentiated hepatocytes, cholangiocytes, and 3D cellular structures derived therefrom. Methods for producing LSC and mature, differentiated hepatocytes and cholangiocytes in culture are provided. Also provided are cell culture systems and cell culture media for producing a homogenous population of liver stem cells that remain in an undifferentiated state over multiple passages in culture. The LSC and methods are useful for producing homogenous populations of hepatocytes and cholangiocytes for downstream applications. The LSC can be transplanted into subjects to treat liver diseases.
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