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公开(公告)号：US12066428B2
公开(公告)日：2024-08-20
申请号：US17083589
申请日：2020-10-29
发明人： Xiaobo Wang , Yama A. Abassi , Nan Li , Lincoln Muir , Xiao Xu
CPC分类号： G01N33/5011 , C12N1/06 , C12N5/0693 , C12N7/00 , G01N27/021 , G01N33/5047 , C12N1/38
摘要： Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.
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公开(公告)号：US20230288429A1
公开(公告)日：2023-09-14
申请号：US18119195
申请日：2023-03-08
发明人： Xiao Xu , Yama A. Abassi , Xiaobo Wang , Jiangbo Gan
IPC分类号： G01N33/68 , C12M1/12 , C12M1/34 , G01N33/50 , G01N33/483
CPC分类号： G01N33/6854 , C12M25/08 , C12M41/36 , G01N33/5005 , G01N33/4836 , G01N2500/10
摘要： Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.
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公开(公告)号：US20230145666A1
公开(公告)日：2023-05-11
申请号：US18095402
申请日：2023-01-10
发明人： Xiao Xu , Yama A. Abassi , Xiaobo Wang , Jiangbo Gan
IPC分类号： G01N33/68 , C12M1/12 , C12M1/34 , G01N33/50 , G01N33/483
CPC分类号： G01N33/6854 , C12M25/08 , C12M41/36 , G01N33/4836 , G01N33/5005 , G01N2500/10
摘要： Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.
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公开(公告)号：US11604197B2
公开(公告)日：2023-03-14
申请号：US16833180
申请日：2020-03-27
发明人： Xiao Xu , Yama A. Abassi , Xiaobo Wang , Jiangbo Gan
IPC分类号： G01N33/68 , C12M1/12 , C12M1/34 , G01N33/50 , G01N33/483
摘要： Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.
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公开(公告)号：US11360072B2
公开(公告)日：2022-06-14
申请号：US16733781
申请日：2020-01-03
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N33/487 , G01N33/543 , G01N27/02
摘要： A system for monitoring cell-substrate impedance of excitable cells at millisecond time resolution and methods of assessing cell beating by monitoring cell-substrate impedance of beating cells at millisecond time resolution.
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公开(公告)号：US11346797B2
公开(公告)日：2022-05-31
申请号：US16721742
申请日：2019-12-19
发明人： Xiaobo Wang , Wei Ouyang , Nan Li , Tianxing Wang , Xiaoyu Zhang , Xiao Xu , Yama A. Abassi
IPC分类号： G01N27/02 , G01N33/483
摘要： Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.
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公开(公告)号：US20210164961A1
公开(公告)日：2021-06-03
申请号：US16733781
申请日：2020-01-03
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N33/487
摘要： A system for monitoring cell-substrate impedance of excitable cells at millisecond time resolution and methods of assessing cell beating by monitoring cell-substrate impedance of beating cells at millisecond time resolution.
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公开(公告)号：US20210041418A1
公开(公告)日：2021-02-11
申请号：US17083589
申请日：2020-10-29
发明人： Xiaobo Wang , Yama A. Abassi , Nan Li , Lincoln Muir , Xiao Xu
IPC分类号： G01N33/50 , G01N33/543 , G01N27/02
摘要： Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.
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公开(公告)号：US20240210379A1
公开(公告)日：2024-06-27
申请号：US18401197
申请日：2023-12-29
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N27/02 , G01N33/487 , G01N33/543
CPC分类号： G01N33/4836 , G01N27/02 , G01N33/4833 , G01N33/48728 , G01N33/5438
摘要： A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.
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公开(公告)号：US11287379B2
公开(公告)日：2022-03-29
申请号：US16833235
申请日：2020-03-27
发明人： Nan Li , Jian Wu , Ye Chen , Tianxing Wang , Xiaobo Wang
摘要： A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.
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