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公开(公告)号：US20240076619A1
公开(公告)日：2024-03-07
申请号：US18361584
申请日：2023-07-28
发明人： Xiaoyu Zhang , Yama A. Abassi , Nan Li , Xiaobo Wang
CPC分类号： C12N5/0657 , C12N5/0018 , C12N5/0696 , C12N13/00 , G01N27/028 , C12N2506/45 , C12N2529/00
摘要： A method of characterizing an effect of a compound on cardiomyocyte beating, including providing a system configured to culture, electrically pace, and monitor heating of beating cells: culturing two populations of immature cardiomyocytes in the system; adding a compound suspected of having an effect on cardiomyocyte maturation to one of the populations of immature cardiomyocytes; electrically pacing the two populations of immature cardiomyocytes according to a pulse profile that functionally matures immature cardiomyocytes until at least one of the two populations of cardiomyocytes is functionally mature; and Characterizing the compound as further driving maturation if the population with compound addition functionally matures before the other cardiomyocyte population.
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公开(公告)号：US20220291191A1
公开(公告)日：2022-09-15
申请号：US17824782
申请日：2022-05-25
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N33/487 , G01N33/543 , G01N27/02
摘要： A method for monitoring a cell beating parameter, which includes adding excitable cells capable of beating to a system for monitoring cell-substrate impedance, the system having an electrode array electrically coupled to an impedance analyzer that measures cell-substrate impedance at millisecond time resolution, and a software program that determines a cell beating parameter from measured cell-substrate impedance; monitoring cell-substrate impedance of the excitable cells; and determining the cell beating parameter from the monitored cell-substrate impedance.
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公开(公告)号：US20240210379A1
公开(公告)日：2024-06-27
申请号：US18401197
申请日：2023-12-29
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N27/02 , G01N33/487 , G01N33/543
CPC分类号： G01N33/4836 , G01N27/02 , G01N33/4833 , G01N33/48728 , G01N33/5438
摘要： A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.
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公开(公告)号：US11346797B2
公开(公告)日：2022-05-31
申请号：US16721742
申请日：2019-12-19
发明人： Xiaobo Wang , Wei Ouyang , Nan Li , Tianxing Wang , Xiaoyu Zhang , Xiao Xu , Yama A. Abassi
IPC分类号： G01N27/02 , G01N33/483
摘要： Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.
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公开(公告)号：US20210164961A1
公开(公告)日：2021-06-03
申请号：US16733781
申请日：2020-01-03
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N33/487
摘要： A system for monitoring cell-substrate impedance of excitable cells at millisecond time resolution and methods of assessing cell beating by monitoring cell-substrate impedance of beating cells at millisecond time resolution.
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公开(公告)号：US20210041418A1
公开(公告)日：2021-02-11
申请号：US17083589
申请日：2020-10-29
发明人： Xiaobo Wang , Yama A. Abassi , Nan Li , Lincoln Muir , Xiao Xu
IPC分类号： G01N33/50 , G01N33/543 , G01N27/02
摘要： Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.
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公开(公告)号：US11906508B2
公开(公告)日：2024-02-20
申请号：US17993716
申请日：2022-11-23
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N33/487 , G01N33/543 , G01N27/02
CPC分类号： G01N33/4836 , G01N27/02 , G01N33/4833 , G01N33/48728 , G01N33/5438
摘要： A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.
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公开(公告)号：US11746328B2
公开(公告)日：2023-09-05
申请号：US16490302
申请日：2018-03-03
发明人： Xiaoyu Zhang , Yama A. Abassi , Nan Li , Xiaobo Wang
CPC分类号： C12N5/0657 , C12N5/0018 , C12N5/0696 , C12N13/00 , G01N27/028 , C12N2506/45 , C12N2529/00
摘要： Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.
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公开(公告)号：US20230130050A1
公开(公告)日：2023-04-27
申请号：US17993716
申请日：2022-11-23
发明人： Xiaobo Wang , Yama A. Abassi , Biao Xi , Wen Fu Zhang , Xiao Xu
IPC分类号： G01N33/483 , G01N33/487 , G01N33/543 , G01N27/02
摘要： A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.
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公开(公告)号：US12066428B2
公开(公告)日：2024-08-20
申请号：US17083589
申请日：2020-10-29
发明人： Xiaobo Wang , Yama A. Abassi , Nan Li , Lincoln Muir , Xiao Xu
CPC分类号： G01N33/5011 , C12N1/06 , C12N5/0693 , C12N7/00 , G01N27/021 , G01N33/5047 , C12N1/38
摘要： Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.
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