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公开(公告)号：US20120004132A1
公开(公告)日：2012-01-05
申请号：US13176669
申请日：2011-07-05
申请人： Aiguo Zhang , Yunqing Ma , Quan N. Nguyen , Franklin R. Witney , Gary K. McMaster , Glenn H. McGall
发明人： Aiguo Zhang , Yunqing Ma , Quan N. Nguyen , Franklin R. Witney , Gary K. McMaster , Glenn H. McGall
IPC分类号： C40B30/04 , G01N33/566 , C12Q1/68
CPC分类号： G01N33/543 , C12Q1/682 , G01N2458/10 , G01N2458/30 , C12Q2525/313
摘要： Methods of detecting various types of nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Detection assays may be conducted at least in vitro, in cellulo, and in situ. Nucleic acids which are optionally captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label probe system with the nucleic acids. Various label probe system embodiments are provided. Embodiments are directed to concurrent detection of one or more nucleic acids and one or more proteins. Embodiments also are directed to determining the methylation state of a target sequence. Other embodiments are directed to detection of one or more proteins using DNA barcodes. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供检测各种类型核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测试验可以至少在体外，纤维素和原位进行。 可以例如通过导致标记探针系统与核酸的特异性缔合的协同杂交事件来检测任选地捕获在固体支持物上的核酸。 提供了各种标签探针系统实施例。 实施方案涉及同时检测一种或多种核酸和一种或多种蛋白质。 实施方案还涉及确定靶序列的甲基化状态。 其他实施方案涉及使用DNA条形码检测一种或多种蛋白质。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20110237449A1
公开(公告)日：2011-09-29
申请号：US12986545
申请日：2011-01-07
CPC分类号： C12Q1/6837 , C12Q2565/514 , C12Q2537/163 , C12Q2525/161
摘要： Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on a substrate, or different subsets of particles, or at different selected positions on a spatially addressable solid support. Methods described include enrichment and purification of nucleic acids prior to downstream steps including sequencing of target nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了从单个样品同时捕获两种或更多种核酸的方法。 不同的核酸通过底物上的协同杂交事件或不同的颗粒子集，或在空间上可寻址的固体支持物上的不同的选定位置捕获。 所描述的方法包括在下游步骤之前的核酸的富集和纯化，包括靶核酸的测序。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20120231453A1
公开(公告)日：2012-09-13
申请号：US13336699
申请日：2011-12-23
申请人： Randall J. True , Yunqing Ma , Aiguo Zhang , Franklin R. Witney
发明人： Randall J. True , Yunqing Ma , Aiguo Zhang , Franklin R. Witney
IPC分类号： C12Q1/68
CPC分类号： B82Y25/00 , B82Y20/00 , G09F3/00 , H01F1/0072
摘要： An encoded microparticle carrying a spatial code is provided; and a set of encoded microparticles are provided with distinguishable spatial codes, wherein the codes comply with a pre-determined coding scheme. Presented are also methods of using the encoded microparticles in various biological assays, such as various multiplex quantitative PCR (real-time PCR) and multiplex chromosomal immunoprecipitation (ChIP) assays.
摘要翻译： 提供携带空间码的编码微粒; 并且一组编码的微粒被提供有可区分的空间代码，其中代码符合预定的编码方案。 还提出了在各种生物测定中使用编码的微粒的方法，例如各种多重定量PCR（实时PCR）和多重染色体免疫沉淀（ChIP）测定。
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公开(公告)号：US5792610A
公开(公告)日：1998-08-11
申请号：US640658
申请日：1996-05-01
CPC分类号： C12Q1/6841 , C12Q1/6816
摘要： The invention relates to a method of identifying the individual autosomal and sex chromosomes of a human karyotype through the use of a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with one predetermined autosomal or sex chromosome of a human karyotype.
摘要翻译： 本发明涉及通过使用一组组合标记的寡核苷酸探针来鉴定人类核型的个体常染色体和性染色体的方法，每一个成员：（i）具有与所述的任何其他成员的标签可区分的预定标签 设置，和（ii）能够与人核型的一个预定的常染色体或性染色体特异性杂交。
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公开(公告)号：US5591981A
公开(公告)日：1997-01-07
申请号：US405468
申请日：1995-03-16
CPC分类号： G01N21/6456 , G01N21/64 , G01N2021/6419 , G01N2021/6421
摘要： A method and apparatus to detect fluorescence from a sample is provided. The optical system allows the user to continuously tune the wavelengths and bandwidths of the excitation source and the emission detection system. A peaking function automatically peaks the detected fluorescent signal by fine tuning the excitation and emission detection systems. A look-up table allows the fluorescent signal for a specific wavelength to be corrected for the wavelength dependence of the optical train. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band, each wavelength band being independently tunable.
摘要翻译： 提供了一种从样品中检测荧光的方法和装置。 光学系统允许用户连续调节激发源和发射检测系统的波长和带宽。 峰值功能通过微调激发和发射检测系统自动使检测到的荧光信号达到峰值。 查找表允许针对光学列车的波长依赖性校正特定波长的荧光信号。 在系统的一个实施例中，样本同时在多于一个波长带中照射，每个波长带是独立可调谐的。
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公开(公告)号：US5784152A
公开(公告)日：1998-07-21
申请号：US729111
申请日：1996-10-11
CPC分类号： G01N21/64 , G01N21/253 , G01N21/6456 , G01N2021/6419 , G01N2021/6421
摘要： A method and apparatus of analyzing samples contained in a microplate is provided. The instrument is capable of measuring fluorescence, luminescence, and/or absorption within multiple locations within a sample well. The instrument is tunable over the excitation and/or detection wavelengths. Neutral density filters are used to extend the sensitivity range of the absorption measuring aspect of the instrument. Due to the wavelength tuning capabilities of the instrument, the spectral dependence of the measured fluorescence, luminescence, and absorption of the materials in question can be analyzed. The combination of a data processor and a look-up table improve the ease of operation of the instrument. Several different formats are available for the output data including creation of a bit map of the sample.
摘要翻译： 提供了分析微孔板中包含的样品的方法和装置。 该仪器能够测量样品井内多个位置的荧光，发光和/或吸收。 仪器可以在激发和/或检测波长上调谐。 中性密度滤波器用于扩展仪器吸收测量方面的灵敏度范围。 由于仪器的波长调谐能力，可以分析所测材料的测量荧光，发光和吸收的光谱依赖性。 数据处理器和查找表的组合可以提高仪器的操作性。 输出数据可以使用几种不同的格式，包括创建样本的位图。
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7.发明授权
公开(公告)号：US5426039A
公开(公告)日：1995-06-20
申请号：US118387
申请日：1993-09-08
CPC分类号： C12N15/1096
摘要： This invention relates to a method of cloning DNA produced by primer extension including PCR amplified, reverse transcriptase-generated or primer extended synthetic DNA. Specifically, it relates to a method in which alkane diol residue containing oligonucleotide primers are incorporated into DNA by primer extension followed by direct cloning of the target DNA. Following transformation, the host excises the alkane diol residue with its endogenous DNA repair machinery.
摘要翻译： 本发明涉及通过引物延伸产生的DNA的克隆方法，包括PCR扩增，逆转录酶产生或引物延伸的合成DNA。 具体而言，涉及通过引物延伸引入含有二醇残基的寡核苷酸引物，直接克隆靶DNA的方法。 转化后，宿主用其内源DNA修复机构切除烷基二醇残基。
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公开(公告)号：US5885531A
公开(公告)日：1999-03-23
申请号：US927556
申请日：1997-09-09
CPC分类号： G01N21/6458 , G01N21/64 , G01N21/6428 , G01N21/6456 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , Y10S435/81
摘要： A method and apparatus of imaging fluorescence in situ hybridization (FISH) is provided. The instrument allows the user to simultaneously acquire images from several different colors. This system, used in conjunction with a combinatorial fluorescence approach, is able to create a FISH karyotype with each chromosome being painted with a different color. The optical system is continuously tunable over the detection wavelengths. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band and the detection system uses a common path interferometer to scan through the detection wavelengths.
摘要翻译： 提供了成像荧光原位杂交（FISH）的方法和装置。 该仪器允许用户同时采集几种不同颜色的图像。 该系统与组合荧光方法结合使用，能够产生FISH染色体核型，每个染色体都涂上不同的颜色。 光学系统在检测波长上连续可调。 在系统的一个实施例中，样本同时在多于一个波长带中照射，并且检测系统使用公共路径干涉仪来扫描检测波长。
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公开(公告)号：US5863504A
公开(公告)日：1999-01-26
申请号：US585303
申请日：1996-01-11
CPC分类号： G01N21/6458 , G01N21/64 , G01N21/6428 , G01N21/6456 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , Y10S435/81
摘要： A method and apparatus of imaging fluorescence in situ hybridization (FISH) is provided. The instrument allows the user to simultaneously acquire images from several different colors. This system, used in conjunction with a combinatorial fluorescence approach, is able to create a FISH karyotype with each chromosome being painted with a different color. The optical system is continuously tunable over the detection wavelengths. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band and the detection system uses a common path interferometer to scan through the detection wavelengths.
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