公开(公告)号：US07097978B2

公开(公告)日：2006-08-29

申请号：US10434947

申请日：2003-05-08

申请人： Alan Wolffe ,  Fyodor Urnov ,  Dmitry Guschin ,  Trevor Collingwood ,  Xiao-Yong Li ,  Brian Johnstone

发明人： Alan Wolffe ,  Fyodor Urnov ,  Dmitry Guschin ,  Trevor Collingwood ,  Xiao-Yong Li ,  Brian Johnstone

IPC分类号： C12Q1/68

CPC分类号： C12N15/1072 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12N15/67 ,  C12Q1/6811 ,  G06F19/22 ,  G06F19/28 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2522/101

摘要： Methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin, are provided.

摘要翻译： 提供了基于药剂对细胞染色质中可及区域的分布和性质的影响来评估药剂的潜在药理作用和/或副作用的方法。

公开(公告)号：US06610489B2

公开(公告)日：2003-08-26

申请号：US09844265

申请日：2001-04-27

申请人： Alan Wolffe ,  Fyodor Urnov ,  Dmitry Guschin ,  Trevor Collingwood ,  Xiao-Yong Li ,  Brian Johnstone

发明人： Alan Wolffe ,  Fyodor Urnov ,  Dmitry Guschin ,  Trevor Collingwood ,  Xiao-Yong Li ,  Brian Johnstone

IPC分类号： C12Q168

CPC分类号： C12N15/1072 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12N15/67 ,  C12Q1/6811 ,  G06F19/22 ,  G06F19/28 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2522/101

摘要： Methods for the identification of a drug target, wherein the drug target is a component of a signal transduction pathway, are provided. The methods are based, in part, on selection of a gene of therapeutic interest and determination of regulatory DNA sequences which regulate expression of the gene in a cell of interest, followed by reconstruction of signal transduction pathways which act on molecules which bind to the regulatory sequence. Also provided are methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin.

公开(公告)号：US06511808B2

公开(公告)日：2003-01-28

申请号：US09844493

申请日：2001-04-27

申请人： Alan Wolffe ,  Fyodor Urnov ,  Dmitry Guschin ,  Trevor Collingwood ,  Xiao-Yong Li ,  Brian Johnstone

发明人： Alan Wolffe ,  Fyodor Urnov ,  Dmitry Guschin ,  Trevor Collingwood ,  Xiao-Yong Li ,  Brian Johnstone

IPC分类号： C12Q168

CPC分类号： G06F19/22 ,  C07K14/4702 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/1034 ,  C12N15/1051 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12Q1/6811 ,  G06F19/28 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2522/101

摘要： Methods for the design of exogenous regulatory molecules, comprising a DNA-binding domain and a functional domain, are provided. The methods rely on the identification, isolation and characterization of regulatory DNA sequences obtained on the basis of their accessibility in cellular chromatin.

公开(公告)号：US08409861B2

公开(公告)日：2013-04-02

申请号：US11304981

申请日：2005-12-15

申请人： Dmitry Guschin ,  Michael C. Holmes

发明人： Dmitry Guschin ,  Michael C. Holmes

IPC分类号： C12N15/00 ,  C12N15/87 ,  C12N15/90

CPC分类号： C12N15/85 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/10

摘要： Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.

摘要翻译： 本文公开了用于靶向缺失双链DNA的方法和组合物。 组合物包括包含切割结构域（或切割半结构域）和工程化锌指结构域的融合蛋白，以及编码它们的多核苷酸。 靶向缺失的方法包括将这种融合蛋白或编码其的多核苷酸引入细胞，从而发生两个目标切割事件。 随后的细胞修复机制导致两个切割位点之间的序列缺失。

公开(公告)号：US20070111215A1

公开(公告)日：2007-05-17

申请号：US11149901

申请日：2005-06-10

申请人： Andrei Mirzabekov ,  Dmitry Guschin ,  Valentine Chik ,  Aleksei Drobyshev ,  Alexander Fotin ,  Gennadiy Yershov ,  Yuri Lysov

发明人： Andrei Mirzabekov ,  Dmitry Guschin ,  Valentine Chik ,  Aleksei Drobyshev ,  Alexander Fotin ,  Gennadiy Yershov ,  Yuri Lysov

IPC分类号： C12Q1/68 ,  C12M1/34 ,  H01L21/00

CPC分类号： C12Q1/6837 ,  B01J2219/00644 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q1/689 ,  C12Q2600/156 ,  G16B25/00 ,  C12Q2565/607 ,  C12Q2565/513

摘要： This invention relates to using customize oligonucleotide microchips as biosensors for the detection and identification of nucleic acids specific for different genes, organisms and/or individuals in the environment, in food and in biological samples. The microchips are designed to convert multiple bits of genetic information into simpler patterns of signals that are interpreted as a unit. Because of an improved method of hybridizing oligonucleotides from samples to microchips, microchips are reusable to transportable. For field study, portable laser or bar code scanners are suitable.

摘要翻译： 本发明涉及使用定制的寡核苷酸微芯片作为生物传感器，用于检测和鉴定在环境中，食物和生物样品中不同基因，生物和/或个体特异性的核酸。 微芯片被设计为将多个遗传信息转换成更简单的信号模式，被解释为一个单位。 由于从样品与微芯片杂交寡核苷酸的改进方法，微芯片可重复使用于可运输。 对于现场研究，便携式激光或条形码扫描仪是合适的。

公开(公告)号：US20060188987A1

公开(公告)日：2006-08-24

申请号：US11304981

申请日：2005-12-15

申请人： Dmitry Guschin ,  Michael Holmes

发明人： Dmitry Guschin ,  Michael Holmes

IPC分类号： C12N5/08 ,  C12N15/87

CPC分类号： C12N15/85 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/10

摘要： Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.