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公开(公告)号：US5705375A
公开(公告)日：1998-01-06
申请号：US253575
申请日：1994-06-03
申请人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Petrus Josephus Maria Van Den Elzen , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons
发明人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Petrus Josephus Maria Van Den Elzen , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons
CPC分类号： C12N15/8245
摘要： The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.
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公开(公告)号：US07033627B2
公开(公告)日：2006-04-25
申请号：US09149310
申请日：1998-02-02
申请人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons , Teunis Cornelis Verwoerd
发明人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons , Teunis Cornelis Verwoerd
CPC分类号： C12Y301/03026 , A21D8/042 , A23K20/189 , A23L29/06 , C12N9/16 , C12N9/2417 , C12N15/8242 , C12N15/8243 , C12N15/8257
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将转基因非野生型种子，优选以研磨形式加入到反应混合物，特别是面包店方法中，并允许种子中的酶提高反应速率。 通过将种子直接加入到反应混合物中，该方法提供了提取和纯化酶的昂贵且有问题的方法的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US06602682B1
公开(公告)日：2003-08-05
申请号：US07711556
申请日：1991-05-29
申请人： Johan Abel Van Den Berg , Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Cornelis P. Hollenberg , Sunil Das , Albert De Leeuw
发明人： Johan Abel Van Den Berg , Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Cornelis P. Hollenberg , Sunil Das , Albert De Leeuw
IPC分类号： C12N1500
CPC分类号： C12N9/2428 , C07K14/43 , C07K14/765 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C12N9/6459 , C12N9/6481 , C12N15/625 , C12N15/68 , C12N15/815 , C12N15/90 , C12Y302/01003 , C12Y304/21069
摘要： Kluyveromyces hosts and DNA expression cassettes for use in Kluyveromyces are provided for transcription of endogenous and/or exogenous DNA, and production of peptides, for enhancing production of an endogenous product, or producing an exogenous product. The Kluyveromyces hosts find particular use for secretion of a desired peptide product, where signal sequences may be native to the peptide or provided from endogenous or exogenous signal sequences, including synthetic sequences, functional in Kluyveromyces. A transformation procedure is provided for efficiently transforming Kluyveromyces.
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公开(公告)号：US5840528A
公开(公告)日：1998-11-24
申请号：US494151
申请日：1995-06-23
IPC分类号： C12N1/19 , C07K14/39 , C12N1/21 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/645 , C12P21/06 , C12N15/11 , C12N15/64
摘要： The present invention discloses a transformed Phaffia strain. Also disclosed are specific vectors for transforming Phaffia, preferably such vectors contain expression regulating sequences obtained from Phaffia. The present invention also discloses the use of certain marker genes in Phaffia. The invention further discloses a method for transforming Phaffia.
摘要翻译： 本发明公开了一种转化的菲菲氏菌。 还公开了用于转化法菲亚的特异性载体，优选地，这些载体含有从菲菲亚得到的表达调节序列。 本发明还公开了在菲律宾中使用某些标记基因。 本发明还公开了一种用于转化Phaffia的方法。
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5.发明授权Cloning and expression of the exo-polygalacturonase gene from aspergillus 失效来自曲霉的外切多聚半乳糖醛酸酶基因的克隆和表达
公开(公告)号：US5830737A
公开(公告)日：1998-11-03
申请号：US780869
申请日：1997-01-24
申请人： Margo Anne-Rose Kusters-Van Someren , Yvonne Muller , Hermanus Cornelis Maria Kester , Jacob Visser , Albert Johannes Joseph Van Ooyen , Claus Rolin
发明人： Margo Anne-Rose Kusters-Van Someren , Yvonne Muller , Hermanus Cornelis Maria Kester , Jacob Visser , Albert Johannes Joseph Van Ooyen , Claus Rolin
CPC分类号： C12Y302/01015 , C12N9/2402
摘要： The exo-polygalacturonase gene from Aspergillus is identified, and the enzyme encoded thereby is expressed. Specifically, the exo-polygalacturonase gene from Aspergillus tubingensis is is cloned and expressed, and DNA sequences from other Aspergillus strains that specifically hybridize therewith are identified. Novel expression vectors that comprise an exo-polygalacturonase encoding sequence, and host cells transformed therewith, are also provided. The invention further relates to the production of recombinant exo-polygalacturonase, and to the use of this protein.
摘要翻译： 鉴定出来自曲霉的外多聚半乳糖醛酸酶基因，并且由此编码的酶被表达。 具体来说，克隆并表达来自曲霉管曲霉的外切多聚半乳糖醛酸酶基因，鉴定与其特异性杂交的其他曲霉菌株的DNA序列。 还提供了包含外切多聚半乳糖醛酸酶编码序列的新型表达载体和用其转化的宿主细胞。 本发明还涉及重组外切聚半乳糖醛酸酶的生产，以及该蛋白质的应用。
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6.发明授权Proteins with Factor VIII activity: process for their preparation using genetically-engineered cells and pharmaceutical compositions containing them 失效具有因子VIII活性的蛋白质：使用遗传工程改造的细胞及其含有它们的药物组合物的制备方法公开(公告)号：US06316226B1
公开(公告)日：2001-11-13
申请号：US08416532
申请日：1995-04-03
申请人： Albert Johannes Joseph Van Ooyen , Hans Pannekoek , Martinus Philippus Verbeet , Robert Willem Van Leen
发明人： Albert Johannes Joseph Van Ooyen , Hans Pannekoek , Martinus Philippus Verbeet , Robert Willem Van Leen
IPC分类号： C12P2100
CPC分类号： C07K16/36 , A61K38/00 , C07K14/755 , C07K2319/00 , C07K2319/02 , C12N15/85 , C12N2830/42 , Y10S530/83
摘要： Novel polypeptides having Factor VIII activity are provided as well as compositions and methods for their preparation. The polypeptides comprise derivatives and fragments of Factor VIII and have sequences substantially similar to portions of naturally occuring Pactor VIII. The polypeptides find use in treatment of Hemophilia A.
摘要翻译： 提供了具有因子VIII活性的新型多肽以及其制备的组合物和方法。 多肽包含因子VIII的衍生物和片段，并且具有与天然存在的Pactor VIII的部分基本相似的序列。 多肽可用于治疗血友病A.
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7.发明授权Proteins with factor VIII activity: process for their preparation using genetically-engineered cells and pharmaceutical compositions containing them 失效具有因子VIII活性的蛋白质：使用遗传工程改造的细胞及其含有它们的药物组合物的制备方法公开(公告)号：US06346513B1
公开(公告)日：2002-02-12
申请号：US08416535
申请日：1995-04-03
申请人： Albert Johannes Joseph Van Ooyen , Hans Pannekoek , Martinus Philippus Verbeet , Robert Willem Van Leen
发明人： Albert Johannes Joseph Van Ooyen , Hans Pannekoek , Martinus Philippus Verbeet , Robert Willem Van Leen
IPC分类号： A61K3800
CPC分类号： C07K14/755 , A61K38/00 , C07K16/36 , C07K2319/00 , C07K2319/02 , C12N15/85 , Y10S514/802 , Y10S514/834
摘要： Novel polypeptides having Factor VIII activity are provided as well as compositions and methods for their preparation. The polypeptides comprise derivatives and fragments of Factor VIII and have sequences substantially similar to portions of naturally occuring Factor VIII. The polypeptides find use in treatment of Hemophilia A.
摘要翻译： 提供了具有因子VIII活性的新型多肽以及其制备的组合物和方法。 多肽包含因子VIII的衍生物和片段，并且具有与天然存在的因子VIII的部分基本相似的序列。 多肽可用于治疗血友病A.
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