公开(公告)号：US5873523A

公开(公告)日：1999-02-23

申请号：US808127

申请日：1997-02-28

申请人： Alessandro Gomez ,  Juan Fernandez de la Mora ,  Keqi Tang

发明人： Alessandro Gomez ,  Juan Fernandez de la Mora ,  Keqi Tang

IPC分类号： A61M15/02 ,  B05B5/025 ,  B05B5/03

CPC分类号： A61M15/02 ,  B05B5/0255 ,  B05B5/03 ,  Y10S977/904 ,  Y10S977/925

摘要： An electrospray apparatus incorporating the invention includes a capillary with an exit for ejecting a fluid, and an electrode positioned downstream from the exit. A gas source is used to establish a region of gas, at least immediately about the exit. A voltage supply creates a potential difference between the capillary exit and the electrode, the potential difference being sufficient to both establish a cone jet mode of operation at the exit of the capillary, to ionize the region of gas about the exit and create a current flow between the exit and the electrode. The current flow exhibits an increased value upon the gas becoming ionized, thus enabling detection of the establishment of a corona. This action creates a corona-assisted cone jet mode of electrospray operation which enables the fluid to disperse into highly uniform sized particles. The preferred gas is carbon dioxide.

摘要翻译： 结合本发明的电喷射装置包括具有用于喷射流体的出口的毛细管和位于出口下游的电极。 气源用于建立气体区域，至少在出口附近。 电压源产生毛细管出口和电极之间的电位差，电势差足以在毛细管出口处建立锥形喷射模式，以使出口周围的气体区域离子化并产生电流 在出口和电极之间。 电流在气体变得电离时表现出增加的值，从而能够检测电晕的建立。 该动作产生电晕辅助锥喷射模式的电喷操作，使得流体能够分散成高度均匀的粒子。 优选的气体是二氧化碳。

公开(公告)号：US08841608B2

公开(公告)日：2014-09-23

申请号：US13861511

申请日：2013-04-12

申请人： Alexandre A. Shvartsburg ,  Keqi Tang ,  Richard D. Smith

发明人： Alexandre A. Shvartsburg ,  Keqi Tang ,  Richard D. Smith

IPC分类号： G01N27/62 ,  H01J49/00

CPC分类号： H01J49/0031 ,  G01N27/622

摘要： A method for raising the resolving power, specificity, and peak capacity of conventional ion mobility spectrometry is disclosed. Ions are separated in a dynamic electric field comprising an oscillatory field wave and opposing static field, or at least two counter propagating waves with different parameters (amplitude, profile, frequency, or speed). As the functional dependencies of mean drift velocity on the ion mobility in a wave and static field or in unequal waves differ, only single species is equilibrated while others drift in either direction and are mobility-separated. An ion mobility spectrum over a limited range is then acquired by measuring ion drift times through a fixed distance inside the gas-filled enclosure. The resolving power in the vicinity of equilibrium mobility substantially exceeds that for known traveling-wave or drift-tube IMS separations, with spectra over wider ranges obtainable by stitching multiple segments. The approach also enables low-cutoff, high-cutoff, and bandpass ion mobility filters.

摘要翻译： 公开了提高常规离子迁移光谱法的分辨能力，特异性和峰值能力的方法。 离子在包括振荡场波和相对静电场的动态电场中分离，或至少两个具有不同参数（幅度，分布，频率或速度）的反向传播波。 由于平均漂移速度对波和静电场或不相等波的离子迁移率的功能依赖性不同，只有单个物质平衡，而其他物质在任一方向上漂移并且是迁移率分离的。 然后通过在充满气体的外壳内的固定距离测量离子漂移时间来获得在有限范围内的离子迁移谱。 在平衡迁移附近的分辨能力基本上超过已知的行波或漂移管IMS分离的分辨能力，其中可以通过缝合多个段获得更宽范围的光谱。 该方法还可实现低截止，高截止和带通离子迁移率滤波器。

公开(公告)号：US20080156978A1

公开(公告)日：2008-07-03

申请号：US11617889

申请日：2006-12-29

申请人： Alexandre A. Shvartsburg ,  Keqi Tang ,  Yehia M. Ibrahim ,  Richard D. Smith

发明人： Alexandre A. Shvartsburg ,  Keqi Tang ,  Yehia M. Ibrahim ,  Richard D. Smith

IPC分类号： H01J49/06

CPC分类号： H01J49/004 ,  G01N27/624

摘要： Disclosed are a device and method for improved interfacing of differential mobility spectrometry (DMS) or field asymmetric waveform ion mobility spectrometry (FAIMS) analyzers of substantially planar geometry to subsequent or preceding instrument stages. Interfacing is achieved using curved DMS elements, where a thick ion beam emitted by planar DMS analyzers or injected into them for ion filtering is compressed to the gap median by DMS ion focusing effect in a spatially inhomogeneous electric field. Resulting thinner beams are more effectively transmitted through necessarily constrained conductance limit apertures to subsequent instrument stages operated at a pressure lower than DMS, and/or more effectively injected into planar DMS analyzers. The technology is synergetic with slit apertures, slit aperture/ion funnels, and high-pressure ion funnel interfaces known in the art which allow for increasing cross-sectional area of MS inlets. The invention may be used in integrated analytical platforms, including, e.g., DMS/MS, LC/DMS/MS, and DMS/IMS/MS that could replace and/or enhance current LC/MS methods, e.g., for proteomics research.

摘要翻译： 公开了一种用于改进基本平面几何形状的差分迁移率谱（DMS）或场非对称波形离子迁移谱（FAIMS）分析仪与随后或之前仪器级的接口的装置和方法。 使用弯曲的DMS元件实现接合，其中通过平面DMS分析仪发射或注入其中以进行离子过滤的厚离子束通过在空间不均匀电场中的DMS离子聚焦效应被压缩到间隙中间值。 所产生的较薄的梁更有效地通过必须约束的电导极限孔传输到在低于DMS的压力下操作的后续仪器级，和/或更有效地注入到平面DMS分析仪中。 该技术与本领域已知的狭缝孔，狭缝孔/离子漏斗和高压离子漏斗接口协同，允许增加MS入口的横截面面积。 本发明可以用于综合分析平台，包括例如DMS / MS，LC / DMS / MS和DMS / IMS / MS，其可以替代和/或增强当前的LC / MS方法，例如蛋白质组学研究。

公开(公告)号：US06818890B1

公开(公告)日：2004-11-16

申请号：US10699006

申请日：2003-10-30

申请人： Richard D. Smith ,  Keqi Tang ,  Alexandre A. Shvartsburg

发明人： Richard D. Smith ,  Keqi Tang ,  Alexandre A. Shvartsburg

IPC分类号： H01J4904

CPC分类号： H01J49/066

摘要： A method and apparatus enabling increased sensitivity in ion mobility spectrometry/mass spectrometry instruments which substantially reduces or eliminates the loss of ions in ion mobility spectrometer drift tubes utilizing an hourglass electrodynamic ion funnel at the entrance to the drift tube and/or an internal ion funnel at the exit of the drift tube. An hourglass electrodynamic funnel is formed of at least an entry element, a center element, and an exit element, wherein the aperture of the center element is smaller than the aperture of the entry element and the aperture of the exit elements. Ions generated in a relatively high pressure region by an ion source at the exterior of the hourglass electrodynamic funnel are transmitted to a relatively low pressure region at the entrance of the hourglass funnel through a conductance limiting orifice. Alternating and direct electrical potentials are applied to the elements of the hourglass electrodynamic funnel thereby drawing ions into and through the hourglass electrodynamic funnel thereby introducing relatively large quantities of ions into the drift tube while maintaining the gas pressure and composition at the interior of the drift tube as distinct from those at the entrance of the electrodynamic funnel and allowing a positive gas pressure to be maintained within the drift tube, if desired. An internal ion funnel is provided within the drift tube and is positioned at the exit of said drift tube. The advantage of the internal ion funnel is that ions that are dispersed away from the exit aperture within the drift tube, such as those that are typically lost in conventional drift tubes to any subsequent analysis or measurement, are instead directed through the exit of the drift tube, vastly increasing the amount of ions exiting the drift tube.

摘要翻译： 一种在离子迁移光谱/质谱仪器中能够提高灵敏度的方法和装置，其基本上减少或消除离子迁移谱仪漂移管中的离子损失，所述漂移管利用在漂移管入口处的沙漏电动力离子漏斗和/或内部离子漏斗 在漂移管的出口处。 沙漏电动漏斗由至少一个入口元件，中心元件和出口元件形成，其中中心元件的孔径小于入口元件的孔径和出口元件的孔径。 在沙漏电动漏斗的外部由离子源在较高压力区域产生的离子通过电导限制孔传输到沙漏漏斗入口处的相对低压区域。 将交替和直接的电势施加到沙漏电动力学漏斗的元件，从而将离子吸入和穿过沙漏电动力学漏斗，从而将相对大量的离子引入漂移管中，同时保持气体压力和组成在漂移管的内部 与电动漏斗的入口处的那些不同，并且如果需要，允许在漂移管内保持正的气体压力。 内部离子漏斗设置在漂移管内并位于所述漂移管的出口处。 内部离子漏斗的优点是离散在漂移管内的出口孔的离子，例如通常在常规漂移管中丢失到任何后续分析或测量中的离子，而是通过漂移出口 管，大大增加离开漂移管的离子的量。

公开(公告)号：US06583408B2

公开(公告)日：2003-06-24

申请号：US09860721

申请日：2001-05-18

申请人： Richard D. Smith ,  Taeman Kim ,  Keqi Tang ,  Harold R. Udseth

发明人： Richard D. Smith ,  Taeman Kim ,  Keqi Tang ,  Harold R. Udseth

IPC分类号： H01J4904

CPC分类号： H01J49/0404 ,  H01J49/066 ,  H01J49/165

摘要： A jet disturber used in combination with an ion funnel to focus ions and other charged particles generated at or near atmospheric pressure into a relatively low pressure region, which allows increased conductance of the ions and other charged particles. The jet disturber is positioned within an ion funnel and may be interfaced with a multi-capillary inlet juxtaposed between an ion source and the interior of an instrument maintained at near atmospheric pressure. The invention finds particular advantages when deployed to improve the ion transmission between an electrospray ionization source and the first vacuum stage of a mass spectrometer.

公开(公告)号：US06528784B1

公开(公告)日：2003-03-04

申请号：US09715815

申请日：2000-11-16

申请人： Keqi Tang ,  Alan E. Schoen ,  Jean-Jacques Dunyach

发明人： Keqi Tang ,  Alan E. Schoen ,  Jean-Jacques Dunyach

IPC分类号： B01D5944

CPC分类号： H01J49/063 ,  H01J49/044 ,  H01J49/067

摘要： There is described an interface for delivering ions generated in an ion source into a mass analyzer in a chamber under vacuum pressure. In particular, the interface employs two consecutive ion guides operated to dissociate adduct ions formed in the ion source or high pressure regions of the interface between the ion source and the mass analyzer, thus improving the limit of detection or limit of quantitation of the mass analyzer by increasing the analyte ion current.

摘要翻译： 描述了在真空压力下将在离子源中产生的离子输送到室中的质量分析器的界面。 特别地，界面使用两个连续的离子导向器来操作以离解形成在离子源中的加合离子或离子源和质量分析器之间的界面的高压区域，从而提高质量分析器的检测限度或定量限度 通过增加分析物离子电流。

公开(公告)号：US20150357174A1

公开(公告)日：2015-12-10

申请号：US14733517

申请日：2015-06-08

申请人： Yehia M. Ibrahim ,  Tsung-Chi Chen ,  Marques B. Harrer ,  Keqi Tang ,  Richard D. Smith

发明人： Yehia M. Ibrahim ,  Tsung-Chi Chen ,  Marques B. Harrer ,  Keqi Tang ,  Richard D. Smith

IPC分类号： H01J49/06

CPC分类号： H01J49/066

摘要： An ion funnel device is disclosed. A first pair of electrodes is positioned in a first direction. A second pair of electrodes is positioned in a second direction. The device includes an RF voltage source and a DC voltage source. A RF voltage with a superimposed DC voltage gradient is applied to the first pair of electrodes, and a DC voltage gradient is applied to the second pair of electrodes.

摘要翻译： 公开了一种离子漏斗装置。 第一对电极位于第一方向。 第二对电极位于第二方向。 该器件包括一个RF电压源和一个直流电压源。 将具有叠加的DC电压梯度的RF电压施加到第一对电极，并且将直流电压梯度施加到第二对电极。

公开(公告)号：US20130299690A1

公开(公告)日：2013-11-14

申请号：US13861511

申请日：2013-04-12

申请人： Alexandre A. Shvartsburg ,  Keqi Tang ,  Richard D. Smith

发明人： Alexandre A. Shvartsburg ,  Keqi Tang ,  Richard D. Smith

IPC分类号： H01J49/00

CPC分类号： H01J49/0031 ,  G01N27/622

摘要： A method for raising the resolving power, specificity, and peak capacity of conventional ion mobility spectrometry is disclosed. Ions are separated in a dynamic electric field comprising an oscillatory field wave and opposing static field, or at least two counter propagating waves with different parameters (amplitude, profile, frequency, or speed). As the functional dependencies of mean drift velocity on the ion mobility in a wave and static field or in unequal waves differ, only single species is equilibrated while others drift in either direction and are mobility-separated. An ion mobility spectrum over a limited range is then acquired by measuring ion drift times through a fixed distance inside the gas-filled enclosure. The resolving power in the vicinity of equilibrium mobility substantially exceeds that for known traveling-wave or drift-tube IMS separations, with spectra over wider ranges obtainable by stitching multiple segments. The approach also enables low-cutoff, high-cutoff, and bandpass ion mobility filters.

摘要翻译： 公开了提高常规离子迁移光谱法的分辨能力，特异性和峰值能力的方法。 离子在包括振荡场波和相对静电场的动态电场中分离，或至少两个具有不同参数（幅度，分布，频率或速度）的反向传播波。 由于平均漂移速度对波和静电场或不相等波的离子迁移率的功能依赖性不同，只有单个物质平衡，而其他物质在任一方向上漂移并且是迁移率分离的。 然后通过在充满气体的外壳内的固定距离测量离子漂移时间来获得在有限范围内的离子迁移谱。 在平衡迁移附近的分辨能力基本上超过已知的行波或漂移管IMS分离的分辨能力，其中可以通过缝合多个段获得更宽范围的光谱。 该方法还可实现低截止，高截止和带通离子迁移率滤波器。

公开(公告)号：US20100207022A1

公开(公告)日：2010-08-19

申请号：US12690818

申请日：2010-01-20

申请人： Keqi Tang ,  Alexandre A. Shvartsburg ,  Richard D. Smith

发明人： Keqi Tang ,  Alexandre A. Shvartsburg ,  Richard D. Smith

IPC分类号： H01J49/04 ,  H01J49/26 ,  H01J49/02

CPC分类号： G01N27/624

摘要： A differential ion mobility spectrometry or field asymmetric waveform ion mobility spectrometry (FAIMS) platform is disclosed that utilizes both gas flow and electric field, consecutively or simultaneously, to move ions through the analytical gap. The consecutive combination of flow and field enables rapid and flexible switching of the FAIMS stage “on” (for ion separation) and “off” (for high non-selective transmission) with no hardware modifications. This capability is needed for effective use of multidimensional instrument systems that couple FAIMS to mass spectrometry and/or conventional ion mobility spectrometry. The joint application of flow and field allows controlling the discrimination against high-mobility ions, maximizing it to remove the chemical noise or minimizing it to make the analyses of complex samples more predictable and uniform.

摘要翻译： 公开了差分离子迁移谱或场非对称波形离子迁移谱（FAIMS）平台，其利用气流和电场连续或同时地将离子移动通过分析间隙。 流程和现场的连续组合使得无需硬件修改即可快速灵活地切换FAIMS阶段（离子分离）和“关闭”（用于高非选择性传输）。 需要这种能力来有效利用将FAIMS耦合到质谱和/或常规离子迁移谱的多维仪器系统。 流量和场的联合应用允许控制对高迁移率离子的鉴别，使其最大化以消除化学噪声或使其最小化，使得复杂样品的分析更可预测和均匀。

公开(公告)号：US20090108194A1

公开(公告)日：2009-04-30

申请号：US11251529

申请日：2005-09-30

申请人： Jason S. Page ,  Keqi Tang ,  Richard D. Smith

发明人： Jason S. Page ,  Keqi Tang ,  Richard D. Smith

IPC分类号： B01D59/44

CPC分类号： H01J49/066 ,  G01N27/622

摘要： An adjustable, low mass-to-charge (m/z) filter is disclosed employing electrospray ionization to block ions associated with unwanted low m/z species from entering the mass spectrometer and contributing their space charge to down-stream ion accumulation steps. The low-mass filter is made by using an adjustable potential energy barrier from the conductance limiting terminal electrode of an electrodynamic ion funnel, which prohibits species with higher ion mobilities from being transmitted. The filter provides a linear voltage adjustment of low-mass filtering from m/z values from about 50 to about 500. Mass filtering above m/z 500 can also be performed; however, higher m/z species are attenuated. The mass filter was evaluated with a liquid chromatography-mass spectrometry analysis of an albumin tryptic digest and resulted in the ability to block low-mass, “background” ions which account for 40-70% of the total ion current from the ESI source during peak elution.

摘要翻译： 公开了一种可调节的低质量 - 电荷（m / z）滤波器，其使用电喷雾电离来阻挡与不想要的低m / z物质相关的离子进入质谱仪并且为下游离子累积步骤贡献其空间电荷。 低质量过滤器通过使用来自电动离子漏斗的电导限制端子电极的可调势势垒来制造，其阻止具有较高离子迁移率的物质被传输。 滤波器提供从大约50到大约500的m / z值的低质量滤波的线性电压调节。也可以执行m / z 500以上的质量过滤; 然而，更高的m / z物种减弱。 通过对白蛋白胰蛋白酶消化物的液相色谱 - 质谱分析来评估质量过滤器，并且导致阻断低质量“背景”离子的能力，其占离子源的总离子电流的40-70％ 峰洗脱。