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公开(公告)号:US20050239205A1
公开(公告)日:2005-10-27
申请号:US10501412
申请日:2003-01-13
CPC分类号: C07K14/005 , C12N7/00 , C12N15/86 , C12N2770/24022 , C12N2770/24221 , C12N2770/24222 , C12N2770/24243 , C12N2840/203 , G01N2333/18 , G01N2500/10
摘要: The present invention features GBV-B replicons and replicon enhanced cells. A GBV-B replicon is an RNA molecule able to autonomously replicate in a cultured cell and produce detectable levels of one or more GBV-B proteins. GBV-B replicon enhanced cells are cells having an increased ability to maintain a GBV-B replicon.
摘要翻译: 本发明具有GBV-B复制子和复制子增强细胞。 GBV-B复制子是能够在培养的细胞中自主复制并产生可检测水平的一种或多种GBV-B蛋白质的RNA分子。 GBV-B复制子增强细胞是具有增加的维持GBV-B复制子的能力的细胞。
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公开(公告)号:US08354518B2
公开(公告)日:2013-01-15
申请号:US10577893
申请日:2004-11-03
申请人: Steven W. Ludmerer , Donald J. Graham , Robert L. LaFemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
发明人: Steven W. Ludmerer , Donald J. Graham , Robert L. LaFemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
CPC分类号: C12Q1/707 , C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222
摘要: The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.
摘要翻译: 本发明的特征在于增强基因型2b NS5B序列在复制子中起作用的能力的方法,用于产生含有功能性基因型2b NS5B的复制子,并且使用复制子来测量化合物影响HCV复制的能力 基因型2b聚合酶。 还有一个基因型1b NS4B自适应突变。 通过产生基于HCV基因型1b的嵌合复制子来说明产生含有功能性基因型2b NS5B的复制子的能力,其中基本上所有NS5B序列被基因型2b NS5B替代。
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公开(公告)号:US20110250628A1
公开(公告)日:2011-10-13
申请号:US10577893
申请日:2004-11-03
申请人: Steven W. Ludmerer , Donald J. Graham , Robert L. Lafemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
发明人: Steven W. Ludmerer , Donald J. Graham , Robert L. Lafemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
CPC分类号: C12Q1/707 , C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222
摘要: The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.
摘要翻译: 本发明的特征在于增强基因型2b NS5B序列在复制子中起作用的能力的方法,用于产生含有功能性基因型2b NS5B的复制子,并且使用复制子来测量化合物影响HCV复制的能力 基因型2b聚合酶。 还有一个基因型1b NS4B自适应突变。 通过产生基于HCV基因型1b的嵌合复制子来说明产生含有功能性基因型2b NS5B的复制子的能力,其中基本上所有NS5B序列被基因型2b NS5B替代。
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