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公开(公告)号:US08354518B2
公开(公告)日:2013-01-15
申请号:US10577893
申请日:2004-11-03
申请人: Steven W. Ludmerer , Donald J. Graham , Robert L. LaFemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
发明人: Steven W. Ludmerer , Donald J. Graham , Robert L. LaFemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
CPC分类号: C12Q1/707 , C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222
摘要: The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.
摘要翻译: 本发明的特征在于增强基因型2b NS5B序列在复制子中起作用的能力的方法,用于产生含有功能性基因型2b NS5B的复制子,并且使用复制子来测量化合物影响HCV复制的能力 基因型2b聚合酶。 还有一个基因型1b NS4B自适应突变。 通过产生基于HCV基因型1b的嵌合复制子来说明产生含有功能性基因型2b NS5B的复制子的能力,其中基本上所有NS5B序列被基因型2b NS5B替代。
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公开(公告)号:US20110250628A1
公开(公告)日:2011-10-13
申请号:US10577893
申请日:2004-11-03
申请人: Steven W. Ludmerer , Donald J. Graham , Robert L. Lafemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
发明人: Steven W. Ludmerer , Donald J. Graham , Robert L. Lafemina , Osvaldo A. Flores , Maura Pizzuti , Cinzia Traboni
CPC分类号: C12Q1/707 , C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222
摘要: The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.
摘要翻译: 本发明的特征在于增强基因型2b NS5B序列在复制子中起作用的能力的方法,用于产生含有功能性基因型2b NS5B的复制子,并且使用复制子来测量化合物影响HCV复制的能力 基因型2b聚合酶。 还有一个基因型1b NS4B自适应突变。 通过产生基于HCV基因型1b的嵌合复制子来说明产生含有功能性基因型2b NS5B的复制子的能力,其中基本上所有NS5B序列被基因型2b NS5B替代。
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公开(公告)号:US07569374B2
公开(公告)日:2009-08-04
申请号:US12002392
申请日:2007-12-17
CPC分类号: C07K14/005 , C07H21/00 , C07H21/04 , C12N9/127 , C12N2770/24222 , G01N2333/18 , G01N2333/9125 , G01N2500/04
摘要: The present invention features NS5B polypeptides from different clinically important HCV genotypes. The polypeptides can be used individually, or as part of a panel of RNA-dependent RNA polymerases, to evaluate the effectiveness of a compound to inhibit NS5B activity.
摘要翻译: 本发明特征来自不同临床重要HCV基因型的NS5B多肽。 多肽可以单独使用或作为一组RNA依赖性RNA聚合酶的一部分使用,以评估化合物抑制NS5B活性的有效性。
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公开(公告)号:US07329732B2
公开(公告)日:2008-02-12
申请号:US10584810
申请日:2005-01-06
CPC分类号: C07K14/005 , C07H21/00 , C07H21/04 , C12N9/127 , C12N2770/24222 , G01N2333/18 , G01N2333/9125 , G01N2500/04
摘要: The present invention features NS5B polypeptides from different clinically important HCV genotypes. The polypeptides can be used individually, or as part of a panel of RNA-dependent RNA polymerases, to evaluate the effectiveness of a compound to inhibit NS5B activity.
摘要翻译: 本发明特征来自不同临床重要HCV基因型的NS5B多肽。 多肽可以单独使用或作为一组RNA依赖性RNA聚合酶的一部分使用,以评估化合物抑制NS5B活性的有效性。
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公开(公告)号:US20080187903A1
公开(公告)日:2008-08-07
申请号:US12002392
申请日:2007-12-17
IPC分类号: C12Q1/00 , C07K14/005 , C07H21/04 , C12N15/63
CPC分类号: C07K14/005 , C07H21/00 , C07H21/04 , C12N9/127 , C12N2770/24222 , G01N2333/18 , G01N2333/9125 , G01N2500/04
摘要: The present invention features NS5B polypeptides from different clinically important HCV genotypes. The polypeptides can be used individually, or as part of a panel of RNA-dependent RNA polymerases, to evaluate the effectiveness of a compound to inhibit NS5B activity.
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公开(公告)号:US07786287B2
公开(公告)日:2010-08-31
申请号:US10510912
申请日:2003-04-11
申请人: Osvaldo A. Flores , Jay Grobler , Edward M. Murray , Paul D. Zuck
发明人: Osvaldo A. Flores , Jay Grobler , Edward M. Murray , Paul D. Zuck
CPC分类号: G01N33/502 , C07K14/005 , C12N15/86 , C12N2730/10122 , C12N2840/203 , C12Q1/34 , C12Q1/6897 , C12Q1/707 , G01N33/5008 , G01N33/5067 , G01N33/5767 , G01N2333/18
摘要: The present invention features assays employing a beta-lactamase reporter system, an HCV replicon enhanced cell, and/or a chimeric HCV replicon containing a 3′ UTR based on the HCV-1a 3′ UTR. These features can be employed alone or together, and are preferably combined together to measure HCV replicon activity and the affect of compounds on such activity.
摘要翻译: 本发明的特征在于使用β-内酰胺酶报道系统,HCV复制子增强细胞和/或含有基于HCV-1a 3'UTR的3'UTR的嵌合HCV复制子。 这些特征可以单独或一起使用,并且优选组合在一起以测量HCV复制子活性和化合物对这种活性的影响。
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公开(公告)号:US08629274B2
公开(公告)日:2014-01-14
申请号:US13723869
申请日:2012-12-21
IPC分类号: C07D213/00 , A01N43/00 , A61K31/445 , A01N41/06 , A61K31/18 , A01N37/18 , A61K31/165
CPC分类号: C07D401/06 , A61K31/167 , A61K31/18 , A61K31/397 , A61K31/40 , A61K31/4015 , A61K31/407 , A61K31/445 , A61K31/4453 , A61K31/451 , A61K31/454 , A61K31/4545 , A61K31/495 , A61K31/496 , A61K31/513 , A61K31/52 , A61K31/522 , A61K31/536 , A61K31/5375 , A61K31/551 , A61K31/675 , A61K31/7072 , A61K38/21 , A61K45/06 , C07C311/46 , C07D205/04 , C07D207/08 , C07D207/09 , C07D207/12 , C07D207/14 , C07D207/16 , C07D207/48 , C07D211/22 , C07D211/32 , C07D211/42 , C07D211/46 , C07D211/48 , C07D211/50 , C07D211/52 , C07D211/58 , C07D211/62 , C07D211/96 , C07D213/74 , C07D213/82 , C07D265/30 , C07D265/32 , C07D295/195 , C07D295/26 , C07D401/12 , A61K2300/00
摘要: The present invention includes a method of inhibiting, suppressing or preventing HBV infection in an individual in need thereof, comprising administering to the individual a therapeutically effective amount of at least one compound of the invention.
摘要翻译: 本发明包括在有需要的个体中抑制,抑制或预防HBV感染的方法,包括向个体施用治疗有效量的至少一种本发明的化合物。
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8.
公开(公告)号:US06183956B2
公开(公告)日:2001-02-06
申请号:US09052995
申请日:1998-03-31
IPC分类号: C12Q168
CPC分类号: C12Q1/6897 , C12Q1/6804
摘要: High-throughput in vitro assays are provided for identifying modulators of transcription activity and RNA expression. New assays, related compositions, apparatus and integrated systems are provided.
摘要翻译: 提供高通量体外测定用于鉴定转录活性和RNA表达的调节剂。 提供了新的测定法,相关组合物,仪器和综合系统。
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