公开(公告)号：US20230069095A1

公开(公告)日：2023-03-02

申请号：US17821835

申请日：2022-08-24

Applicant: AMGEN INC.

Inventor： Elaheh Binabaji ,  Chio Mui Chan ,  Hadley Krizner ,  Junfen Ma ,  Diana Woehle

IPC: C07K16/28 ,  C07K1/113 ,  C07K1/22 ,  B01D15/38 ,  C07K16/42 ,  C07K16/06

Abstract: The present disclosure relates to methods of re-oxidizing an antigen-binding protein.

公开(公告)号：US11098079B2

公开(公告)日：2021-08-24

申请号：US15751231

申请日：2016-08-12

Applicant: AMGEN INC.

Inventor： Hai Hoang ,  Rafael Gonzalez ,  Junfen Ma

IPC: C07K1/36 ,  C07K1/18 ,  C07K1/113 ,  C07K1/34 ,  B01D15/36 ,  B01D15/38 ,  C07K1/22 ,  C07K16/28 ,  G01N27/447

Abstract: Methods of producing an aqueous formulation of an antigen-binding protein or enhancing re-oxidation of an antigen-binding protein are disclosed. The methods comprise (a) contacting an aqueous solution comprising antigen-binding protein molecules with a charged depth filter under conditions sufficient to enhance re-oxidation of the antigen-binding protein molecules and achieve a decrease in the percentage of reduced antigen-binding protein molecules, compared to the percentage of reduced antigen-binding protein molecules observed prior to step (a); and (b) optionally, measuring the amount or relative amount of reduced antigen-binding protein molecules. Formulations comprising a re-oxidized antigen-binding protein are also described.

公开(公告)号：US20250163154A1

公开(公告)日：2025-05-22

申请号：US19022273

申请日：2025-01-15

Applicant: AMGEN INC.

Inventor： Junfen Ma ,  Elaheh Binabaji ,  Chio Mui Chan ,  Hadley Krizner ,  Diana Woehle

IPC: C07K16/28 ,  B01D15/38 ,  C07K1/113 ,  C07K1/22 ,  C07K16/06 ,  C07K16/42

Abstract: The present disclosure relates to methods of re-oxidizing an antigen-binding protein.

公开(公告)号：US20210363180A1

公开(公告)日：2021-11-25

申请号：US17386692

申请日：2021-07-28

Applicant: AMGEN INC.

Inventor： Hai Hoang ,  Rafael Gonzalez ,  Junfen Ma

IPC: C07K1/36 ,  C07K1/113 ,  C07K1/34 ,  B01D15/36 ,  B01D15/38 ,  C07K1/18 ,  C07K1/22 ,  C07K16/28

Abstract: Methods of producing an aqueous formulation of an antigen-binding protein or enhancing re-oxidation of an antigen-binding protein are disclosed. The methods comprise (a) contacting an aqueous solution comprising antigen-binding protein molecules with a charged depth filter under conditions sufficient to enhance re-oxidation of the antigen-binding protein molecules and achieve a decrease in the percentage of reduced antigen-binding protein molecules, compared to the percentage of reduced anti-gen-binding protein molecules observed prior to step (a); and (b) optionally, measuring the amount or relative amount of reduced an¬tigen-binding protein molecules. Formulations comprising a re-oxidized antigen-binding protein are also described.

公开(公告)号：US20180230180A1

公开(公告)日：2018-08-16

申请号：US15751231

申请日：2016-08-12

Applicant: AMGEN INC.

Inventor： Hai Hoang ,  Rafael Gonzalez ,  Junfen Ma

IPC: C07K1/36 ,  C07K1/34 ,  C07K1/22 ,  C07K1/113 ,  C07K1/18 ,  C07K16/28 ,  B01D15/38 ,  B01D15/36

CPC classification number: C07K1/36 ,  B01D15/362 ,  B01D15/3809 ,  C07K1/1133 ,  C07K1/18 ,  C07K1/22 ,  C07K1/34 ,  C07K16/2887 ,  C07K2317/40 ,  G01N27/44791

Abstract: Methods of producing an aqueous formulation of an antigen-binding protein or enhancing re-oxidation of an antigen-binding protein are disclosed. The methods comprise (a) contacting an aqueous solution comprising antigen-binding protein molecules with a charged depth filter under conditions sufficient to enhance re-oxidation of the antigen-binding protein molecules and achieve a decrease in the percentage of reduced antigen-binding protein molecules, compared to the percentage of reduced antigen-binding protein molecules observed prior to step (a); and (b) optionally, measuring the amount or relative amount of reduced antigen-binding protein molecules. Formulations comprising a re-oxidized antigen-binding protein are also described.

公开(公告)号：US11447547B1

公开(公告)日：2022-09-20

申请号：US16219871

申请日：2018-12-13

Applicant: Amgen Inc.

Inventor： Elaheh Binabaji ,  Chio Mui Chan ,  Hadley Krizner ,  Junfen Ma ,  Diana Woehle

IPC: C07K1/113 ,  C07K1/22 ,  C07K16/06 ,  B01D15/38 ,  C07K16/28 ,  C07K16/42

Abstract: The present disclosure relates to methods of re-oxidizing an antigen-binding protein.

公开(公告)号：US10023608B1

公开(公告)日：2018-07-17

申请号：US14208043

申请日：2014-03-13

Applicant: AMGEN INC.

Inventor： Junfen Ma ,  Xiaoyang Zhao ,  Brian Williamson

IPC: C07K1/20 ,  C07K16/24 ,  C07K19/00 ,  C07K1/16

Abstract: Disclosed are methods of purifying a protein sample. More specifically, provide are methods of removing or reducing the amount of high molecular weight species and/or high mannose species from a protein sample using a mixed mode chromatography step and a hydrophobic interaction chromatography step.