Oxidation of carbohydrates by means of peroxidases and nitroxy radicals
    2.
    发明授权
    Oxidation of carbohydrates by means of peroxidases and nitroxy radicals 失效
    通过过氧化物酶和硝酰自由基氧化碳水化合物

    公开(公告)号:US07517675B2

    公开(公告)日:2009-04-14

    申请号:US10583434

    申请日:2004-12-17

    CPC分类号: C12P19/02 C12P7/58 C12P17/04

    摘要: A method of oxidising carbohydrates and/or carbohydrate derivatives having primary alcohol groups comprising contacting a reaction medium containing said carbohydrates and/or carbohydrate derivatives with a nitroxy radical mediator and a peroxidase enzyme, characterized in that the initial reaction medium contains at least 10% by weight carbohydrates and/or carbohydrate derivatives, in that the peroxidase enzyme is an oilseed peroxidase and in that a hydroperoxide and an alkali compound are gradually added to the reaction medium such that its pH is maintained at between 3.5 and 10.0 (pH-Stat); use of said method in a process for producing gluconic acid, glucaric acid and/or D-glucuronolactone.

    摘要翻译: 一种氧化具有伯醇基团的碳水化合物和/或碳水化合物衍生物的方法,包括使含有所述碳水化合物和/或碳水化合物衍生物的反应介质与硝基氧基介质和过氧化物酶接触,其特征在于,初始反应介质包含至少10% 重量的碳水化合物和/或碳水化合物衍生物,因为过氧化物酶是油脂过氧化物酶,并且将氢过氧化物和碱性化合物逐渐加入到反应介质中,使得其pH保持在3.5至10.0(pH-Stat)之间; 在生产葡糖酸,葡糖二酸和/或D-葡糖醛酸内酯的方法中使用所述方法。

    Purified acid-stable alpha-amylase from fungal origin
    5.
    发明授权
    Purified acid-stable alpha-amylase from fungal origin 失效
    从真菌来源纯化的酸稳定的α-淀粉酶

    公开(公告)号:US5962276A

    公开(公告)日:1999-10-05

    申请号:US24579

    申请日:1998-02-17

    摘要: The present invention describes a simple and inexpensive purification method for acid-stable fungal alpha-amylase wherein the alpha-amylase is obtained without significant loss of enzyme activity. The purified acid-stable alpha-amylase which is obtained is substantially free from glucoamylase. The method for obtaining an acid-stable alpha-amylase comprises the steps ofadjusting the pH of the enzyme containing solution to a value between 1 and 8,heating the solution to a temperature and for a time sufficient to inactivate glucoamylase,removing the denatured glucoamylase.The purified acid-stable alpha-amylase is used for the conversion of starch. The purified alpha-amylase is also used in an immobilized form.

    摘要翻译: 本发明描述了一种简单而廉价的酸稳定型真菌α-淀粉酶纯化方法,其中获得的α-淀粉酶没有明显的酶活性损失。 获得的纯化的酸稳定的α-淀粉酶基本上不含葡糖淀粉酶。 获得酸稳定性α-淀粉酶的方法包括以下步骤:将含酶溶液的pH调节至1至8之间的值,将溶液加热至足以灭活葡糖淀粉酶的温度和时间,除去变性葡糖淀粉酶 。 纯化的酸稳定的α-淀粉酶用于淀粉的转化。 纯化的α-淀粉酶也以固定化形式使用。