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公开(公告)号：US11028400B2
公开(公告)日：2021-06-08
申请号：US15750792
申请日：2016-06-17
申请人： KIKKOMAN CORPORATION
发明人： Keiichi Ichikawa , Yasutomo Shinohara , Seiichi Hara , Keiko Kurosawa , Yumiko Yamashita , Michiaki Yamashita
IPC分类号： C12P17/10 , C12N9/88 , C12N9/02 , C12N1/14 , C12N15/63 , C12R1/665 , C12R1/685 , C12R1/69 , C12N15/09 , C12R1/66
摘要： The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).
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公开(公告)号：US5962276A
公开(公告)日：1999-10-05
申请号：US24579
申请日：1998-02-17
CPC分类号： C12N9/2417 , C12N9/242 , C12P19/14 , C13K7/00 , Y10S435/914 , Y10S435/917
摘要： The present invention describes a simple and inexpensive purification method for acid-stable fungal alpha-amylase wherein the alpha-amylase is obtained without significant loss of enzyme activity. The purified acid-stable alpha-amylase which is obtained is substantially free from glucoamylase. The method for obtaining an acid-stable alpha-amylase comprises the steps ofadjusting the pH of the enzyme containing solution to a value between 1 and 8,heating the solution to a temperature and for a time sufficient to inactivate glucoamylase,removing the denatured glucoamylase.The purified acid-stable alpha-amylase is used for the conversion of starch. The purified alpha-amylase is also used in an immobilized form.
摘要翻译： 本发明描述了一种简单而廉价的酸稳定型真菌α-淀粉酶纯化方法，其中获得的α-淀粉酶没有明显的酶活性损失。 获得的纯化的酸稳定的α-淀粉酶基本上不含葡糖淀粉酶。 获得酸稳定性α-淀粉酶的方法包括以下步骤：将含酶溶液的pH调节至1至8之间的值，将溶液加热至足以灭活葡糖淀粉酶的温度和时间，除去变性葡糖淀粉酶 。 纯化的酸稳定的α-淀粉酶用于淀粉的转化。 纯化的α-淀粉酶也以固定化形式使用。
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3.发明授权Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in aspergillus 失效加工重组乳铁蛋白和乳铁蛋白多肽片段从曲霉菌中融合产物的表达
公开(公告)号：US5955316A
公开(公告)日：1999-09-21
申请号：US691123
申请日：1996-08-01
IPC分类号： C12N15/09 , A61K38/00 , C07K14/79 , C12N1/15 , C12N15/12 , C12N15/80 , C12P21/02 , C12R1/665 , C12R1/69 , C12N15/62 , C12N15/64
CPC分类号： C12N15/80 , C07K14/79 , A61K38/00 , C07K2319/02
摘要： The subject invention provides for the production of lactoferrins and lactoferrin polypeptide fragments using the host cells Aspergillus in combination with novel plasmid constructs. More specifically, the subject invention provides novel vector constructs capable of producing lactoferrins and lactoferrin polypeptide fragments in Aspergillus host cells. More particularly, the subject invention provides for novel plasmid constructs suitable for use with Aspergillus and especially Aspergillus awamori, niger and oryzae host cells, which enables them to produce large amounts of recombinant lactoferrins and lactoferrin polypeptide fragments.
摘要翻译： 本发明提供使用宿主细胞曲霉与新型质粒构建体组合产生乳铁蛋白和乳铁蛋白多肽片段。 更具体地，本发明提供能够在曲霉属宿主细胞中产生乳铁蛋白和乳铁蛋白多肽片段的新型载体构建体。 更具体地，本发明提供了适用于曲霉属，特别是泡盛曲霉，黑曲霉和谷氨酸宿主细胞的新型质粒构建体，其使得它们能够产生大量的重组乳铁蛋白和乳铁蛋白多肽片段。
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4.发明授权公开(公告)号：US10711290B2
公开(公告)日：2020-07-14
申请号：US15533215
申请日：2015-12-25
申请人： KIKKOMAN CORPORATION
发明人： Seiichi Hara , Keiko Kurosawa , Keiichi Ichikawa
摘要： The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.
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公开(公告)号：US5372940A
公开(公告)日：1994-12-13
申请号：US859439
申请日：1992-06-02
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
IPC分类号： C12N9/18 , C12P7/42 , C12P17/04 , C12P41/00 , C12R1/645 , C12R1/665 , C12R1/77 , C12R1/82 , C12R1/845 , C12P17/02 , C12N9/14
CPC分类号： C12P7/42 , C12N9/18 , C12P17/04 , C12P41/005
摘要： The invention is drawn to a new enzyme that selectively hydrolyzes D-pantolactone in D,L-pantolactone and has the following characteristics:(a) action: acts on pantolactone to produce the corresponding acid;(b) specificity for substrate: acts specifically on D-pantolactone but not on L-pantolactone;(c) pH stability: stable at pH5-9;(d) optimal pH: 7.0-7.5;(e) optimal temperature: ca. 50.degree. C.; and(f) effect of various metal ions or inhibitors: inhibited by Cd.sup.2+, Hg.sup.2+ or Cu.sup.2+ EDTA, as well as a process for the preparation thereof. The enzyme is preferably produced by cultivating the microorganisms of the genera Fusarium, Cylindrocarpon, and Gibberella, and most preferably the microorganism Fusarium oxysporum IFO 5942.
摘要翻译： PCT No.PCT / JP91 / 01351 Sec。 371日期：1992年6月2日 102（e）日期1992年6月2日PCT提交1991年10月4日PCT公布。 公开号WO92 / 06182 日期：1992年4月16日。本发明涉及一种在D，L-泛酸内酯中选择性水解D-泛酸内酯的新酶，具有以下特征：（a）作用：作用于泛酸内酯以产生相应的酸; （b）底物的特异性：特异性地对D-泛酸内酯作用，但不对L-泛酸内酯作用; （c）pH稳定性：pH5-9时稳定; （d）最佳pH：7.0-7.5; （e）最佳温度：约 50℃。 和（f）各种金属离子或抑制剂的作用：被Cd2 +，Hg2 +或Cu2 + EDTA抑制，以及其制备方法。 该酶优选通过栽培镰刀菌属，圆锥菌属和赤霉属的微生物，最优选微生物尖孢镰刀菌IFO 5942来产生。
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公开(公告)号：US5015579A
公开(公告)日：1991-05-14
申请号：US13446
申请日：1987-02-10
申请人： Takamasa Yamaguchi , Ikuo Nogami
发明人： Takamasa Yamaguchi , Ikuo Nogami
CPC分类号： C07D303/48 , C12P17/02 , Y10S435/911 , Y10S435/913 , Y10S435/916 , Y10S435/932
摘要： A method for producing (-)trans-2, 3-epoxysuccinic acid, which can be used as a good starting material for the sythesis of optically active compounds such as optically specific single .beta.-lactam antibiotics, characterized in that filamentous fungi capable of producing (-)trans-2,3-epoxysuccinic acid are cultured in liquid medium while either ammonia, sodium hydroxide or potassium hydroxide is added to maintain the culture medium in a pH range of 5.0 to 7.5 throughout the culturing period.
摘要翻译： 用于制备（ - ）反式-2,3-环氧琥珀酸的方法，其可以用作光学活性化合物如光学特异性单β-内酰胺抗生素合成的良好起始原料，其特征在于能够产生丝状真菌 （ - ）反式-2,3-环氧琥珀酸在液体培养基中培养，同时加入氨，氢氧化钠或氢氧化钾，以便在整个培养期间将培养基保持在5.0至7.5的pH范围内。
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7.发明授权公开(公告)号：US10760107B2
公开(公告)日：2020-09-01
申请号：US15533215
申请日：2015-12-25
申请人： KIKKOMAN CORPORATION
发明人： Seiichi Hara , Keiko Kurosawa , Keiichi Ichikawa
摘要： The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.
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公开(公告)号：US20180237815A1
公开(公告)日：2018-08-23
申请号：US15750792
申请日：2016-06-17
申请人： KIKKOMAN CORPORATION
发明人： Keiichi ICHIKAWA , Yasutomo SHINOHARA , Seiichi HARA , Keiko KUROSAWA , Yumiko YAMASHITA , Michiaki YAMASHITA
摘要： The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).
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9.发明授权Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus 失效加工重组乳铁蛋白和乳铁蛋白多肽片段在曲霉中的融合产物的表达
公开(公告)号：US6080559A
公开(公告)日：2000-06-27
申请号：US107075
申请日：1998-06-29
IPC分类号： C12N15/09 , A61K38/00 , C07K14/79 , C12N1/15 , C12N15/12 , C12N15/80 , C12P21/02 , C12R1/665 , C12R1/69 , C07K14/00 , C12N15/62
CPC分类号： C12N15/80 , C07K14/79 , A61K38/00 , C07K2319/02
摘要： The subject invention provides for the production of lactoferrins and lactoferrin polypeptide fragments using the host cells Aspergillus in combination with novel plasmid constructs. More specifically, the subject invention provides novel vector constructs capable of producing lactoferrins and lactoferrin polypeptide fragments in Aspergillus host cells. More particularly, the subject invention provides for novel plasmid constructs suitable for use with Aspergillus and especially Aspergillus awamori, niger and oryzae host cells, which enables them to produce large amounts of recombinant lactoferrins and lactoferrin polypeptide fragments.
摘要翻译： 本发明提供使用宿主细胞曲霉与新型质粒构建体组合产生乳铁蛋白和乳铁蛋白多肽片段。 更具体地，本发明提供能够在曲霉属宿主细胞中产生乳铁蛋白和乳铁蛋白多肽片段的新型载体构建体。 更具体地，本发明提供了适用于曲霉属，特别是泡盛曲霉，黑曲霉和谷氨酸宿主细胞的新型质粒构建体，其使得它们能够产生大量的重组乳铁蛋白和乳铁蛋白多肽片段。
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公开(公告)号：US4734368A
公开(公告)日：1988-03-29
申请号：US654070
申请日：1984-09-24
申请人： Fritz Schindler
发明人： Fritz Schindler
CPC分类号： C12P7/46 , Y10S435/911 , Y10S435/914 , Y10S435/92 , Y10S435/929 , Y10S435/932 , Y10S435/933
摘要： L-malic acid is produced in a concentration of 170 to 400 g per liter and high yield by biotechnical conversion of fumaric acid neutralized with ammonium hydroxide in nutrient-free solution or suspension. Pure L-malic acid can be obtained economically therefrom with high efficiency in a quality suitable for food and pharmaceutical use. The fermentation can be carried out in simple vessels under non-sterile conditions. The conversion rate of the fumarate and the attainable concentration of L-malate are promoted by the ammonium ions.
摘要翻译： L-苹果酸以170至400g /升的浓度生产，并且通过生物技术转化在无营养物溶液或悬浮液中用氢氧化铵中和的富马酸的高产率。 经济地获得纯L-苹果酸可以高效地获得适合于食品和药物用途的质量。 发酵可以在非无菌条件下的简单容器中进行。 富马酸盐的转化率和L-苹果酸的可达到的浓度由铵离子促进。
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