-
公开(公告)号：US07537920B2
公开(公告)日：2009-05-26
申请号：US10547447
申请日：2004-01-29
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译： 本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。 有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。 本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸 构建体和/或载体。 本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。
-
公开(公告)号：US08486671B2
公开(公告)日：2013-07-16
申请号：US12417171
申请日：2009-04-02
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
-
公开(公告)号：US20090209774A1
公开(公告)日：2009-08-20
申请号：US12417171
申请日：2009-04-02
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译： 本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。 有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。 本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸 构建体和/或载体。 本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。
-
公开(公告)号：US20060168687A1
公开(公告)日：2006-07-27
申请号：US10547447
申请日：2004-01-29
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into said organism which code for polypeptides having acyl-CoA:lysophospholipid acyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising said nucleic acid sequences and/or said nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译： 本发明涉及通过将核酸引入所述生物体中来生产多不饱和脂肪酸的方法，其编码具有酰基-CoA：溶血磷脂酰基转移酶活性的多肽。 有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。 本发明还涉及包含本发明的核酸序列的核酸构建体的核酸序列与包含所述核酸序列和/或所述核酸构建体的载体以及包含上述核酸序列的转基因生物体，核酸 构建体和/或载体。 本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。
-
公开(公告)号：US20080155705A1
公开(公告)日：2008-06-26
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
-
公开(公告)号：US09433228B2
公开(公告)日：2016-09-06
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceæ such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
-
7.发明授权Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids 有权植物酰基转移酶特异于长链，多不饱和脂肪酸公开(公告)号：US08354569B2
公开(公告)日：2013-01-15
申请号：US12844938
申请日：2010-07-28
申请人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
发明人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids coding for polypeptides with acyltransferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, and constructs, vectors and organisms comprising the nucleic acid sequences. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含核酸序列的核酸序列和构建体，载体和生物体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。
-
8.发明授权Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids 有权植物酰基转移酶特异于长链，多不饱和脂肪酸公开(公告)号：US07855321B2
公开(公告)日：2010-12-21
申请号：US10552013
申请日：2004-03-26
申请人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
发明人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The present invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which code for polypeptides with acyl transferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences which code for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适的话，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含本发明的核酸序列的核酸序列，核酸构建体，载体和生物，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物 ，核酸构建体和/或载体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。
-
9.发明授权Production of polyunsaturated fatty acids, novel biosynthesis genes, and novel plant expression constructs 有权生产多不饱和脂肪酸，新型生物合成基因和新型植物表达构建体公开(公告)号：US08729337B2
公开(公告)日：2014-05-20
申请号：US13342142
申请日：2012-01-02
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.
摘要翻译： 本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。 本发明还涉及在该方法中编码具有去饱和酶或延长酶活性的多肽的核酸序列SEQ ID NO：1,3,5,9和11的用途，并用于产生转基因生物，优选转基因植物或转基因微生物 ，具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油脂或脂质以及它们的同系物或衍生物，包括这些基因的基因构建物的含量增加，以及它们单独使用或与生物合成相结合 多不饱和脂肪酸的基因。 本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。
-
10.发明申请Production of Polyunsaturated Fatty Acids, Novel Biosynthesis Genes, And Novel Plant Expression Constructs 有权多不饱和脂肪酸的生产，新型生物合成基因和新型植物表达构建体公开(公告)号：US20120210465A1
公开(公告)日：2012-08-16
申请号：US13342142
申请日：2012-01-02
申请人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
发明人： Jens Lerchl , Andreas Renz , Ernst Heinz , Frederic Domergue , Ulrich Zähringer
IPC分类号： C12P7/64 , C12N1/13 , C12N1/21 , C12N1/11 , C12N5/10 , C07H21/04 , C12N1/19 , C12N9/02 , C12N9/00 , C07C69/007 , A01H5/00 , A01H1/06 , C12N15/63 , C12N15/52
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.
摘要翻译： 本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。 本发明还涉及在该方法中编码具有去饱和酶或延长酶活性的多肽的核酸序列SEQ ID NO：1,3,5,9和11的用途，并用于产生转基因生物，优选转基因植物或转基因微生物 ，具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油脂或脂质以及它们的同系物或衍生物，包括这些基因的基因构建物的含量增加，以及它们单独使用或与生物合成相结合 多不饱和脂肪酸的基因。 本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。
-
-
-
-
-
-
-
-
-
搜索结果
34 条记录 (耗时 0.034 秒)
