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    Pollen Preferred Promoters and Methods of Use
    1.
    发明申请
    Pollen Preferred Promoters and Methods of Use 有权
    花粉优选的启动子和使用方法

    公开(公告)号：US20130180006A1

    公开(公告)日：2013-07-11

    申请号：US13445288

    申请日：2012-04-12

    申请人： Andrew Mark Cigan ,  Shai J. Lawit

    发明人： Andrew Mark Cigan ,  Shai J. Lawit

    IPC分类号： C12N15/82 ,  A01H5/10 ,  C12N5/10

    CPC分类号： C12N15/8231 ,  C12N9/1077 ,  C12N9/22 ,  C12N15/8261 ,  C12N15/8263 ,  C12Y204/0203 ,  C12Y301/27

    摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.

    摘要翻译： 提供了用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。 还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

    Method to Screen Plants for Genetic Elements Inducing Parthenogenesis in Plants
    2.
    发明申请
    Method to Screen Plants for Genetic Elements Inducing Parthenogenesis in Plants 审中-公开
    筛选植物遗传元素诱导植物中孤雌生殖的方法

    公开(公告)号：US20130180005A1

    公开(公告)日：2013-07-11

    申请号：US13445276

    申请日：2012-04-12

    申请人： Andrew Mark Cigan ,  Shai J. Lawit

    发明人： Andrew Mark Cigan ,  Shai J. Lawit

    IPC分类号： A01H1/06 ,  A01H5/10 ,  A01H5/00

    CPC分类号： C12N15/8287 ,  C12N15/8233

    摘要： Compositions and methods for producing a plant population lacking sexually derived embryos are provided. Compositions include suppression cassettes encoding polynucleotides and promoters resulting in parthenogenesis. Further provided are parthenogenesis genetic elements used to prevent sexual reproduction in self-reproducing plants.Methods include: utilizing maternal embryo defective recessive mutations which are maintained as a sterile inbred maintenance system, allowing generation of populations that are homozygous for recessive mutant alleles, but transgenically complemented. Methods include utilizing a toxin genes expressed via egg-cell specific promoters, creating a dominant, embryo-less phenotypes, non-transmittable through female gametes. Resultant hemizygous plants are transformed with egg-cell promoters driving the antidote, a pollen ablation PTU and a seed color marker for identification of transgenic seed. The generation of a plants 50% female fertile, having seed which when grown in the next generation will yield plants with 50% viable transgenic seed, and 50% non-viable embryo-less seed.

    摘要翻译： 提供了缺乏性衍生胚胎的植物群体的组合物和方法。 组合物包括编码多核苷酸的抑制盒和产生单性生成的启动子。 进一步提供的是孤雌生殖遗传元件，用于防止自我繁殖植物中的有性繁殖。 方法包括：利用作为无菌近交维持系统保持的母体胚胎缺陷隐性突变，允许产生对于隐性突变体等位基因是纯合的群体，但是经过补充。 方法包括利用通过卵细胞特异性启动子表达的毒素基因，产生显性的无胚胎表型，通过雌性配子不可透过。 用驱动解毒剂的卵细胞启动子，花粉消融PTU和用于鉴定转基因种子的种子颜色标记物转化所产生的半合子植物。 一代植物50％的女性可育，有种子，当下一代种植时，将产生具有50％活力的转基因种子和50％不能存活的无胚芽种子的植物。