摘要:
By substituting biomass with a surface-active substance in a fermentation broth containing amino acid before spray-drying, granulated animal feed additives with improved product specifications were obtained.
摘要:
The invention relates to a microorganism that produces and/or secretes an organic chemical compound, wherein the microorganism has an increased expression, compared to the respective starting strain, of a polypeptide LpdA with the activity of a transhydrogenase; and a process for producing an organic chemical compound using the microorganism according to the invention.
摘要:
The invention relates to a microorganism which produces and/or secretes an organic-chemical compound, wherein the microorganism has increased expression, compared to the particular starting strain, of one or more protein subunits of the ABC transporter having the activity of a trehalose importer, said microorganism being capable of taking up trehalose from the medium; and to a method for the production of an organic-chemical compound, using the microorganism according to the invention, wherein accumulation of trehalose in the fermentation broth is reduced or avoided.
摘要:
The invention relates to a microorganism that produces and/or secretes an organic chemical compound, wherein the microorganism has an increased expression, compared to the respective starting strain, of a polypeptide LpdA with the activity of a transhydrogenase; and a process for producing an organic chemical compound using the microorganism according to the invention.
摘要:
The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
摘要:
An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.
摘要翻译:分离的多核苷酸编码包含SEQ ID NO:2的氨基酸序列的多肽,氨基酸序列的第5位的L-天冬氨酸被另一种蛋白原性氨基酸替代,并具有柠檬酸合酶活性。 此外,载体包含多核苷酸,细菌包含载体。 分离的多核苷酸包含从位置1-39包含对应于SEQ ID NO:11的位置1-39,位置40至105的核苷酸序列的核苷酸序列,编码SEQ ID NO: 12,除了L-天冬氨酸以外的各蛋白质氨基酸存在于位置5.还描述了L-氨基酸的生产方法。
摘要:
The present invention is directed to bacteria which contain a lysCFBR that encodes a feedback resistant aspartokinase enzyme and whose expression is under the control of a gleceraldehyde-3-phosphate dehydrogenase (gap) promoter. The bacteria may be used in the fermentative production of L-amino acids, especially, L-lysine.
摘要:
The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
摘要:
The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.
摘要:
The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.
摘要翻译:本发明涉及一种制备有机化合物的方法,其特征在于进行以下步骤:a)分泌L-氨基酸的微生物的发酵,该微生物含有编码具有多磷酸依赖性多肽的过表达多核苷酸 NAD +激酶活性,在发酵培养基中形成发酵液,b)所述化合物在所述发酵液中和/或所述微生物细胞中的积累。 本发明涉及通过发酵其中具有多磷酸依赖性NAD +激酶的多肽被过表达的微生物来制备有机化合物的方法。