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    METHOD FOR FERMENTATIVELY PREPARING L-AMINO ACIDS
    6.
    发明申请
    METHOD FOR FERMENTATIVELY PREPARING L-AMINO ACIDS 有权
    用于发酵制备L-氨基酸的方法

    公开(公告)号：US20120164698A1

    公开(公告)日：2012-06-28

    申请号：US13379654

    申请日：2010-06-18

    申请人： Volker F. Wendisch ,  Steffen Lindner ,  Brigitte Bathe ,  Wilfried Claes

    发明人： Volker F. Wendisch ,  Steffen Lindner ,  Brigitte Bathe ,  Wilfried Claes

    IPC分类号： C12P13/04 ,  C12P13/12 ,  C12N1/21 ,  C12P13/22 ,  C12N15/54 ,  C12P13/08 ,  C12P13/06

    CPC分类号： C12P13/08 ,  C12N9/1205 ,  C12Y207/01023

    摘要： The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.

    摘要翻译： 本发明涉及一种制备有机化合物的方法，其特征在于进行以下步骤：a）分泌L-氨基酸的微生物的发酵，该微生物含有编码具有多磷酸依赖性多肽的过表达多核苷酸 NAD +激酶活性，在发酵培养基中形成发酵液，b）所述化合物在所述发酵液中和/或所述微生物细胞中的积累。 本发明涉及通过发酵其中具有多磷酸依赖性NAD +激酶的多肽被过表达的微生物来制备有机化合物的方法。

    METHOD OF PRODUCTION OF L-AMINO ACIDS
    8.
    发明申请
    METHOD OF PRODUCTION OF L-AMINO ACIDS 有权
    生产L-氨基酸的方法

    公开(公告)号：US20100261257A1

    公开(公告)日：2010-10-14

    申请号：US12750968

    申请日：2010-03-31

    申请人： Brigitte BATHE ,  Wilfried Claes

    发明人： Brigitte BATHE ,  Wilfried Claes

    IPC分类号： C12N1/21 ,  C07H21/04 ,  C12N15/77

    CPC分类号： C12N9/88 ,  C12N9/1217 ,  C12P13/06 ,  C12P13/08 ,  C12Y203/03001 ,  C12Y207/02004

    摘要： An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.

    摘要翻译： 分离的多核苷酸编码包含SEQ ID NO：2的氨基酸序列的多肽，氨基酸序列的第5位的L-天冬氨酸被另一种蛋白原性氨基酸替代，并具有柠檬酸合酶活性。 此外，载体包含多核苷酸，细菌包含载体。 分离的多核苷酸包含从位置1-39包含对应于SEQ ID NO：11的位置1-39，位置40至105的核苷酸序列的核苷酸序列，编码SEQ ID NO： 12，除了L-天冬氨酸以外的各蛋白质氨基酸存在于位置5.还描述了L-氨基酸的生产方法。