公开(公告)号：US20200011778A1

公开(公告)日：2020-01-09

申请号：US16482661

申请日：2018-02-07

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  ADVANCED TEAR DIAGNOSTICS

Inventor： Mackenzie Honikel ,  Chi Lin ,  Andrew Penman ,  Brittney Cardinell ,  Jeffrey LaBelle ,  Pierce Youngbar ,  Marcus Smith

IPC: G01N13/04 ,  G01N33/487 ,  G01N33/493 ,  G01N33/49

Abstract: Osmolality and osmolality sensors and methods utilizing electrochemical impedance to detect changes in impedance to varying salinity concentrations. By way of example, the impedance reported at the specified frequency varies logarithmically with the concentration of sodium chloride subject to the sensor surface. Measurements obtained by the sensors and methods herein are utilized, for example, to differentiate between the clinical stages of dry eye disease (290-316 mOsm/L) to complement the current diagnostic procedures. Blood serum, urinalysis, and saliva also may be tested and the corresponding osmolarity or osmolality level evaluated for indications of a disease or condition.

公开(公告)号：US20170234894A1

公开(公告)日：2017-08-17

申请号：US15503364

申请日：2015-07-27

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Jeffrey LaBelle ,  Tina Hakimi ,  Brittney Cardinell

IPC: G01N33/74 ,  G01N27/02 ,  G01N27/327 ,  G01N33/543 ,  G01N27/30

CPC classification number: G01N27/327 ,  A61B5/053 ,  A61B5/14507 ,  A61B5/14546 ,  A61B5/165 ,  A61B2562/0215 ,  G01N27/026 ,  G01N27/301 ,  G01N27/3273 ,  G01N33/5436 ,  G01N33/5438 ,  G01N33/743 ,  G01N2800/7004

Abstract: Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as Cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N-ethylcarbodiimide and 10 mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73 pM and less than 10% relative standard deviation.

公开(公告)号：US11747330B2

公开(公告)日：2023-09-05

申请号：US16543400

申请日：2019-08-16

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Jeffrey LaBelle ,  Tina Hakimi ,  Brittney Cardinell

IPC: G01N33/543 ,  G01N27/327 ,  G01N27/02 ,  A61B5/145 ,  G01N27/30 ,  G01N33/74 ,  A61B5/053 ,  A61B5/16

CPC classification number: G01N33/5438 ,  A61B5/14507 ,  A61B5/14546 ,  G01N27/026 ,  G01N27/301 ,  G01N27/327 ,  G01N27/3273 ,  G01N33/5436 ,  G01N33/743 ,  A61B5/053 ,  A61B5/165 ,  A61B2562/0215 ,  G01N2800/7004

Abstract: Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N-ethylcarbodiimide and 10 mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73 pM and less than 10% relative standard deviation.

公开(公告)号：US10386321B2

公开(公告)日：2019-08-20

申请号：US15503364

申请日：2015-07-27

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Jeffrey LaBelle ,  Tina Hakimi ,  Brittney Cardinell

IPC: G01N27/327 ,  G01N33/543 ,  A61B5/145 ,  G01N27/02 ,  G01N27/30 ,  G01N33/74 ,  A61B5/053 ,  A61B5/16

Abstract: Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as Cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N-ethylcarbodiimide and 10 mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73 pM and less than 10% relative standard deviation.